1887

Abstract

We previously reported that the A/Leningrad/134/47/57 (H2N2) cold-adapted virus (A/Len/47) used in preparing reassortant live attenuated vaccines for children acquired 14 (11 coding) mutations in genes coding for proteins other than haemagglutinin and neuraminidase during cold-adaptation. Preservation of these mutations in genomes of viruses isolated from children on the second, fifth, or eighth day after vaccination was examined by sequence analysis. The sequence data demonstrated that all nine coding mutations selected for examination were conserved in the genomes of all 11 strains investigated, indicating that the mutations accompanying cold-adaptation and attenuation of the A/Len/47 master vaccine are highly stable.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-6-1521
1995-06-01
2022-05-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/6/JV0760061521.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-6-1521&mimeType=html&fmt=ahah

References

  1. Alexandrova G. I. 1971; Basic trends in vaccination of children against influenza by use of live vaccines. In Proceedings of the Symposium on Live Influenza Vaccine pp 121–127 Zagreb: Yugoslav Academy of Sciences and Arts;
    [Google Scholar]
  2. Alexandrova G. I., Polezhaev F. I., Budilovsky G. N., Garmashova L. M., Topuria N. A., Egorov A. Y., Romejko-Gurko Y. R., Koval T. A., Lisovskaya K. V., Klimov A. I., Ghendon Y. Z. 1984; Recombinant cold-adapted attenuated influenza A vaccines for use in children reactogenicity and antigenic activity of cold-adapted recombinants and analysis of isolates from the vaccinees. Infection and Immunity 44:734–739
    [Google Scholar]
  3. Alexandrova G. I., Budilovsky G. N., Koval T. A., Polezhaev H., Garmashova L. M., Ghendon Y. Z., Romanova Y. R., Smorodintsev A. A. 1986; Study of live recombinant cold-adapted influenza bivalent vaccine of type A for use in children: an epidemiological control trial. Vaccine 4:114–118
    [Google Scholar]
  4. Domingo E., Holland J. J. 1988; High error rates, population equilibrium and evolution of RNA replication systems. In RNA Genetics vol 3 pp 3–36 Edited by Domingo E., Holland J. J., Ahlguist P. Boca Raton: CRC Press;
    [Google Scholar]
  5. Ghendon Y. Z., Polezhaev F. I., Lisovskaya K. V., Medvedeva T. E., Alexandrova G. I., Klimov A. I. 1984; Recombinant cold-adapted attenuated influenza A vaccines for use in children: molecular genetic analysis of the cold-adapted donor and recombinants. Infection and Immunity 44:730–733
    [Google Scholar]
  6. Kendal A. P., Maassab H. F., Alexandrova G. I., Ghendon Y. Z. 1981; Development of cold-adapted recombinant live, attenuated influenza A vaccines in U.S.A. and U.S.S.R. Antiviral Research 1:339–365
    [Google Scholar]
  7. Klimov A. I., Cox N. J., Yotov W. V., Rocha E., Alexandrova H., Kendal A. P. 1992; Sequence changes in the live attenuated, cold-adapted variants of influenza A/Leningrad/134/57 (H2N2) virus. Virology 186:795–797
    [Google Scholar]
  8. Medvedeva T. E., Romanova Y. R., Guschina M. I., Rudenko L. G., Ghendon Y. Z., Alexandrova G. I., Klimov A. I. 1990; Ts-phenotype of isolates from children vaccinated with live cold-adapted influenza type A vaccine. Voprosi Virusologii [Russian] 35:101–105
    [Google Scholar]
  9. Rudenko L. G., Slepushkin A. N., Monto A. S., Kendal A. P., Grigorieva E. P., Burtseva E. P., Rekstin A. R., Beljaev A. L., Bragina V. E., Cox N. J., Ghendon Y. Z., Alexandrova G. I. 1993; Efficacy of live attenuated and inactivated influenza vaccines in school children and their unvaccinated contacts in Novgorod, Russia. Journal of Infectious Diseases 168:881–887
    [Google Scholar]
  10. Steinhoff M. C., Halsey N. A., Fries L. F., Wilson M. H., King J., Burns B. A., Samorodin R. K., Perkis V., Murphy B. R., Clements M. L. 1991; The A/Mallard/6750/78 avian-human, but not the A/Ann Arbor/6/60 cold-adapted, influenza A/Kawasaki/86 (H1N1) reassortant virus vaccine retains partial virulence for infants and children. Journal of Infectious Diseases 163:1023–1028
    [Google Scholar]
  11. Treanor J. J., Tierney E. L., London W. T., Murphy B. R. 1991; Characterization of the attenuating M and NP gene segments of the avian influenza A/Mallard/78 virus during in vitro production of avian-human reassortant vaccine viruses and after replication in humans and primates. Vaccine 9:495–499
    [Google Scholar]
  12. Zhdanov V. M. 1986; Live influenza vaccines in USSR: development and practical application. In Options for the Control of Influenza pp 193–205 UCLA Symposia on Molecular and Cellular Biology, no.36 Edited by Kendal A., Patriarca P. New York: A. R. Liss;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-6-1521
Loading
/content/journal/jgv/10.1099/0022-1317-76-6-1521
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error