1887

Abstract

A putative movement protein of molecular mass 50 kDa encoded by the ORF2 of the apple chlorotic leaf spot virus (ACLSV) genome was expressed in using an expression vector and was then used to produce an antiserum. Immunoblot analysis using an antiserum raised against this protein showed that the ORF2 protein of ACLSV was detected in both cell wall and cell membrane fractions prepared from infected tissues. The ORF2 protein from infected tissues had a molecular mass of 52 kDa, larger than that of the full-length ORF2 protein (50 kDa protein) expressed in . Incubation of the 52 kDa protein with alkaline phosphatase resulted in a decrease in its apparent molecular mass from 52 kDa to 50 kDa, strongly suggesting that the ORF2 protein of ACLSV is phosphorylated in infected plant tissues.

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/content/journal/jgv/10.1099/0022-1317-76-6-1503
1995-06-01
2024-04-25
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References

  1. Albrecht H., Geldreich A., Menissier de Murcia J., Kirchherr D., Mesnard J.-M., Lebeurier G. 1988; Cauliflower mosaic virus gene I product detected in a cell-wall-enriched fraction. Virology 163:503–508
    [Google Scholar]
  2. Atkins D., Roberts K., Hull R., Prehaud C., Bishop D. H. L. 1991; Expression of the tobacco mosaic virus movement protein using a baculovirus expression vector. Journal of General Virology 72:2831–2835
    [Google Scholar]
  3. Berna A., Briand J.-P., Stussi-Garaud C., Godefroy-Colburn T. 1986; Kinetics of accumulation of the three non-structural proteins of alfalfa mosaic virus in tobacco plants. Journal of General Virology 67:1135–1147
    [Google Scholar]
  4. Citovsky V., Macean B. G., Zupan J. R., Zambryski P. 1993; Phosphorylation of tobacco mosaic virus cell-to-cell movement protein by a developmentally regulated plant cell wall-associated protein kinase. Genes & Development 7:904–910
    [Google Scholar]
  5. Eggen R., Kaan A., Goldbach R., Van Kammen A. 1988; Cowpea mosaic virus RNA replication in crude membrane fractions from infected cowpea and Chenopodium amaranticolor. Journal of General Virology 69:2711–2720
    [Google Scholar]
  6. Faye L., Chrispeels M. J. 1985; Characterization of A-linked oligosaccharides by affinoblotting with concanavalin A-peroxidase and treatment of the blots with glycosidases. Analytical Biochemistry 149:218–224
    [Google Scholar]
  7. German S., Candresse T., Lanneau M., Huet J. C., Pernollet J. C., Dunez J. 1990; Nucleotide sequence and genome organization of apple chlorotic leaf spot closterovirus. Virology 179:104–112
    [Google Scholar]
  8. Godefroy-Colburn T., Gagey M.-J., Berna A., Stussi-Garaud C. 1986; A non-structural protein of alfalfa mosaic virus in the walls of infected tobacco cells. Journal of General Virology 67:2233–2239
    [Google Scholar]
  9. Kormelink R., Storms M., Van Lent J., Peters D., Goldbach R. 1994; Expression and subcellular location of the NSM protein of tomato spotted wilt virus (TSWV), a putative viral movement protein. Virology 200:56–65
    [Google Scholar]
  10. Martelli G. P., Candresse T., Namba S. 1993; Trichovirus, a new genus of plant viruses. Archives of Virology 134:451–455
    [Google Scholar]
  11. Melcher U. 1990; Similarities between putative transport proteins of plant viruses. Journal of General Virology 71:1009–1018
    [Google Scholar]
  12. Moser O., Gagey M.-J., Godefroy-Colburn T., Stussi-Garaud C., Ellwart-Tschurtz M., Nitschko H., Mundry K.-W. 1988; The fate of the transport protein of tobacco mosaic virus in systemic and hypersensitive tobacco hosts. Journal of General Virology 69:1367–1373
    [Google Scholar]
  13. Mushegian A. R., Koonin E. V. 1993; Cell-to-cell movement of plant viruses. Insights from amino acid sequence comparisons of movement proteins and from analogies with cellular transport systems. Archives of Virology 133:239–257
    [Google Scholar]
  14. Osman T. A. M., Buck K. W. 1991; Detection of the movement protein of red clover necrotic mosaic virus in a cell wall fraction from infected Nicotiana clevelandii plants. Journal of General Virology 72:2853–2856
    [Google Scholar]
  15. Sato K., Yoshikawa N., Takahashi T. 1993; Complete nucleotide sequence of the genome of an apple isolate of apple chlorotic leaf spot virus. Journal of General Virology 74:1927–1931
    [Google Scholar]
  16. Yanase H. 1974; Studies on apple latent viruses in Japan. Bulletin of the Fruit Tree Research Station, Japan Series C1 47–109
    [Google Scholar]
  17. Yoshikawa N., Takahashi T. 1988; Properties of RNAs and proteins of apple stem grooving and apple chlorotic leaf spot viruses. Journal of General Virology 69:241–245
    [Google Scholar]
  18. Yoshikawa N., Sasaki E., Kato M., Takahashi T. 1992; The nucleotide sequence of apple stem grooving capillovirus genome. Virology 19198–105
    [Google Scholar]
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