@article{mbs:/content/journal/jgv/10.1099/0022-1317-76-6-1497, author = "Legorburu, F. J. and Robinson, D. J. and Torrance, L. and Duncan, G. H.", title = "Antigenic analysis of nematode-transmissible and non-transmissible isolates of tobacco rattle tobravirus using monoclonal antibodies", journal= "Journal of General Virology", year = "1995", volume = "76", number = "6", pages = "1497-1501", doi = "https://doi.org/10.1099/0022-1317-76-6-1497", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-76-6-1497", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Murine monoclonal antibodies (MAbs) were produced against a nematode non-transmissible isolate of tobacco rattle tobravirus (TRV-PLB). Two of the MAbs (SCR 80 and 81) did not react with the serologically closely related isolate TRV-PPK20 (both isolates belong to the PRN serotype), which is readily transmissible by nematodes. When further isolates of the PRN serotype were tested, all the non-transmissible ones reacted with these two MAbs, but so also did some of those that are readily transmissible. SCR 80 and 81 were able to trap TRV-PLB particles onto electron microscope grids and reacted with metatopes located near the C terminus of the particle protein. The epitope recognized by SCR 81 was discontinuous. The MAbs that reacted with both TRV-PLB and TRV-PPK20 recognized either continuous cryptotopes (SCR 78) or discontinuous neotopes (SCR 79 and 82).", }