1887

Abstract

Murine monoclonal antibodies (MAbs) were produced against a nematode non-transmissible isolate of tobacco rattle tobravirus (TRV-PLB). Two of the MAbs (SCR 80 and 81) did not react with the serologically closely related isolate TRV-PPK20 (both isolates belong to the PRN serotype), which is readily transmissible by nematodes. When further isolates of the PRN serotype were tested, all the non-transmissible ones reacted with these two MAbs, but so also did some of those that are readily transmissible. SCR 80 and 81 were able to trap TRV-PLB particles onto electron microscope grids and reacted with metatopes located near the C terminus of the particle protein. The epitope recognized by SCR 81 was discontinuous. The MAbs that reacted with both TRV-PLB and TRV-PPK20 recognized either continuous cryptotopes (SCR 78) or discontinuous neotopes (SCR 79 and 82).

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-6-1497
1995-06-01
2022-05-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/6/JV0760061497.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-6-1497&mimeType=html&fmt=ahah

References

  1. Altschuh D., Al Moudallal Z., Briand J. P., Van Regenmortel M. H. V. 1985; Immunochemical studies on tobacco mosaic virus-VI. Attempts to localize viral epitopes with monoclonal antibodies. Molecular Immunology 22:329–337
    [Google Scholar]
  2. Angenent G. C., Linthorst H. J. M., van Belkum A. F., Cornelissen B. J. C., Bol J. F. 1986; RNA 2 of tobacco rattle virus strain TCM encodes an unexpected gene. Nucleic Acids Research 14:4673–1682
    [Google Scholar]
  3. Angenent G. C., Postumus E., Brederode F. H., Bol J. 1989; Genome structure of tobacco rattle virus strain PLB: further evidence on the occurrence of RNA recombination among tobraviruses. Virology 171:271–274
    [Google Scholar]
  4. Bergh S. T., Koziel M. G., Huang S.-C., Thomas R. A., Gilley D. P., Siegel A. 1985; The nucleotide sequence of tobacco rattle virus RNA-2 (CAM strain). Nucleic Acids Research 13:8507–8518
    [Google Scholar]
  5. Dore I., Weiss E., Altschuh D., Van Regenmortel M. H. V. 1988; Visualization by electron microscopy of the location of tobacco mosaic virus epitopes reacting with monoclonal antibodies in enzyme immunoassay. Virology 162:279–289
    [Google Scholar]
  6. Farmer M. J. 1989; Studies on monoclonal antibodies to the particle proteins of a potyvirus and a nepovirus. PhD Thesis University of Dundee;
    [Google Scholar]
  7. Geysen H. M., Meloen R. B., Barteling S. J. 1984; Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid. Proceedings of the National Academy of Sciences, USA 81:998–1002
    [Google Scholar]
  8. Goulden M. G., Lomonossoff G. P., Davies J. W., Wood K. R. 1990; The complete nucleotide sequence of PEBV RNA-2 reveals the presence of a novel open reading frame and provides insights into the structure of tobraviral subgenomic promoters. Nucleic Acids Research 18:4507–4515
    [Google Scholar]
  9. Harrison B. D., Robinson D. J. 1986; Tobraviruses. In The Plant Viruses vol 2 pp 339–369 Edited by van Regenmortel M. H. V., Fraenkel-Conrat H. New York: Plenum;
    [Google Scholar]
  10. Mowat W. P., Dawson S. 1987; Detection and identification of plant viruses by ELISA using crude sap extracts and unfractionated antisera. Journal of Virological Methods 15:233–247
    [Google Scholar]
  11. Offord R. E. 1966 Studies on plant viruses Ph.D. Thesis University of Cambridge;
    [Google Scholar]
  12. Pereira L. G., Torrance L., Roberts I. M., Harrison B. D. 1994; Antigenic structure of the coat protein of potato mop-top furovirus. Virology 203:277–285
    [Google Scholar]
  13. Ploeg A. T., Brown D. F. J., Robinson D. J. 1992a; Acquisition and subsequent transmission of tobacco rattle virus isolates by Paratrichodorus and Trichodorus nematode species. Netherlands Journal of Plant Pathology 98:291–300
    [Google Scholar]
  14. Ploeg A. T., Brown D. J. F., Robinson D. J. 1992b; The association between species of Trichodorus and Paratrichodorus vector nematodes and serotypes of tobacco rattle tobravirus. Annals of Applied Biology 121:619–630
    [Google Scholar]
  15. Torrance L. 1992; Serological methods to detect plant viruses: production and use of monoclonal antibodies. In Techniques for the Detection of Plant Pathogens pp 7–33 Edited by Duncan J. M., Torrance L. Oxford: Blackwell;
    [Google Scholar]
  16. Van Regenmortel M. H. V. 1966; Plant virus serology. Advances in Virus Research 12:207–271
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-6-1497
Loading
/content/journal/jgv/10.1099/0022-1317-76-6-1497
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error