1887

Abstract

We have cloned, sequenced and analysed an additional five circular ssDNA components of banana bunchy top virus (BBTV) which we have called components 2, 3, 4, 5 and 6. These components were present in all BBTV infections tested. Four of these components (components 3, 4, 5 and 6) had one large open reading frame (ORF) in the virion sense located 3′ of a stem-loop structure. Each ORF had a potential TATA box and one or two potential polyadenylation signals associated with it and each polyadenylation signal had an associated GC-rich region containing the trinucleotide sequence TTG. A number of ORFs were identified in component 2 but none of these had appropriately located potential TATA boxes and polyadenylation signals associated with them. None of the ORF amino acid sequences nor the full DNA sequences of any of the components had significant sequence identity with any known protein or nucleic acid sequences. However, the ORF of component 4 encoded a 30 residue hydrophobic domain which may indicate that this ORF encoded a trans-membrane protein. Further, the ORFs of components 3 and 5 potentially encoded proteins of about 20 kDa, the size of the BBTV coat protein. There were two regions of sequence identity between the five components described here and the previously described component 1. Each component contained a conserved stem-loop structure and a nonanucleotide potential TATA box which was 5′ of the large virion-sense ORF in five of the components. The stem-loop structures were incorporated in a common region (CR-SL) of 69 nucleotides which was 62% identical between components. All six BBTV components also contained a major common region (CR-M) which was located 5′ of the CR-SL in each component, in the non-coding region and was 76% identical over 92 nucleotides. Each CR-M contained a near-complete 16 nucleotide direct repeat and a GC-box which was similar to the rightward promoter element found in wheat dwarf geminivirus. From these results, BBTV appears to belong to an undescribed plant virus group which could also include subterranean clover stunt virus, coconut foliar decay virus, faba bean necrotic yellows virus and milk vetch dwarf virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-6-1471
1995-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/6/JV0760061471.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-6-1471&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic logical alignment search tool. Journal of Molecular Biology 215:403–410
    [Google Scholar]
  2. Boulton M. I., Pallaghy C. K., Chatani M., MacFarlane S., Davies J. W. 1993; Replication of maize streak virus mutants in maize protoplasts: evidence for a movement protein. Virology 192:85–93
    [Google Scholar]
  3. Bucher P. 1990; Weight matrix of four eukaryotic RNA polymerase II promoter elements derived from 502 unrelated promoter sequences. Journal of Molecular Biology 212:563–578
    [Google Scholar]
  4. Burns T. M., Harding R. M., Dale J. L. 1994; Evidence that banana bunchy top virus has a multiple component genome. Archives of Virology 137:371–380
    [Google Scholar]
  5. Chu P. W. G., Keese P., Qiu B. S., Waterhouse P. M., Gerlach W. L. 1990; Novel ssDNA genome organization of a new plant virus. Vlllth International Congress of Virology Berlin: August, 1990. Abstract W82–001
    [Google Scholar]
  6. Chu P. G. W., Keese P., Qiu B.-S., Waterhouse P. M., Gerlach W. L. 1993; Putative full-length clones of the genomic DNA segments of subterranean clover stunt virus and identification of the segment coding for the viral coat protein. Virus Research 27:161–171
    [Google Scholar]
  7. Conway L., Wickens M. 1985; A sequence downstream of A-A-U-A-A-A is required for formation of simian virus 40 late mRNA 3′ termini in frog oocytes. Proceedings of the National Academy of Sciences, USA 82:3949–3953
    [Google Scholar]
  8. Dale J. L. 1987; Banana bunchy top: an economically important tropical plant virus disease. Advances in Virus Research 33:301–325
    [Google Scholar]
  9. Fenoll C., Schwarz J. J., Black D. M., Schneider M., Howell S. H. 1990; The intergenic region of maize streak virus contains a GC-rich element that activates rightward transcription and binds maize nuclear factors. Plant Molecular Biology 15:865–877
    [Google Scholar]
  10. Francki R. I. B., Randles J. W. 1973; Some properties of lettuce necrotic yellows virus RNA and its in vitro transcription by virion-associated transcriptase. Virology 54:359–368
    [Google Scholar]
  11. Gil A., Proudfoot N. J. 1984; A sequence downstream of AAUAAA is required for rabbit β-globm mRNA 3′-end formation. Nature 312:473–474
    [Google Scholar]
  12. Gubler U., Hoffman B. J. 1983; An improved and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  13. Hanahan D. 1983; Studies on the transformation of Escherichia coli with plasmids. Journal of Molecular Biology 166:557–580
    [Google Scholar]
  14. Harding R. M., Burns T. M., Dale J. L. 1991; Virus-like particles associated with banana bunchy top disease contain small single-stranded DNA. Journal of General Virology 72:225–230
    [Google Scholar]
  15. Harding R. M., Burns T. M., Hafner G., Dietzgen R. G., Dale J. L. 1993; Nucleotide sequence of one component of the banana bunchy top virus genome contains a putative replicase gene. Journal of General Virology 74:323–328
    [Google Scholar]
  16. Hattori M., Sakaki Y. 1986; Dideoxynucleotide sequencing method using denatured plasmid templates. Analytical Biochemistry 152:232–238
    [Google Scholar]
  17. Heyraud F., Matzeit V., Kammann M., Schaefer S., Schell J., Gronenborn B. 1993; Identification of the initiation sequence for viral-strand DNA synthesis of wheat dwarf virus. EMBO Journal 12:4445–1452
    [Google Scholar]
  18. Higgins D. G., Bleasby A. J., Fuchs R. 1991; CLUSTAL V: improved software for multiple sequence alignment. CABIOS 8:189–191
    [Google Scholar]
  19. Karan M., Harding R. M., Dale J. L. 1994; Evidence for two groups of banana bunchy top virus isolates. Journal of General Virology 75:3541–3546
    [Google Scholar]
  20. Katul L., Vetten H. J., Maiss E., Makkouk K. M., Lesemann D.-E., Casper R. 1993; Characterization and serology of viruslike particles associated with faba bean necrotic yellows. Annals of Applied Biology 123:629–647
    [Google Scholar]
  21. Lazarowitz S. G. 1992; Geminiviruses: genome structure and gene function. Critical Reviews in Plant Sciences 11:327–349
    [Google Scholar]
  22. Mankertz A., Blass G., Mankertz J., Walter A., Bulk H. J. 1993; Analysis of transcription and replication of porcine circo virus (PCV). IXth International Congress of Virology Glasgow: August, 1993. Abstract W45–8
    [Google Scholar]
  23. Marchuk D., Drumm M., Saulino A., Collins F. S. 1990; Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Nucleic Acids Research 19:1154
    [Google Scholar]
  24. Noteborn M. M. H., van der Eb A. J., Koch G. 1993; Molecular characterization of chicken anaemia virus (CAV). IXth International Congress of Virology Glasgow: August, 1993. Abstract W45–7
    [Google Scholar]
  25. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proceedings of the National Academy of Sciences, USA 85:2444–2448
    [Google Scholar]
  26. Phenix K. V., Meehan B., Todd D., McNulty M. S. 1993; Genomic organization of chicken anaemia virus. IXth International Congress of Virology Glasgow: August, 1993. Abstract P45–3
    [Google Scholar]
  27. Ritchie B. W., Niagro F. D., Lukert P. D., Steffens W. L. III, Latimer K. S. 1989; Characterization of a new virus from cockatoos with psittacine beak and feather disease. Virology 171:83–88
    [Google Scholar]
  28. Rohde W., Randles J. W., Langridge P., Hanold D. 1990; Nucleotide sequence of a circular single-stranded DNA associated with coconut foliar decay virus. Virology 176:648–651
    [Google Scholar]
  29. Sambrook J., Maniatis T., Fritsch E. F. 1989 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  30. Sano Y., Isogai M., Satoh S., Koiima K. 1993; Small virus-like particles containing single-stranded DNAs associated with milk vetch dwarf disease in Japan. 6th International Congress of Plant Pathology Montreal: August, 1993. Abstract 17.1.27
    [Google Scholar]
  31. Saunders K., Lucy A., Stanley J. 1993; DNA forms of the geminivirus -African cassava mosaic virus -consistent with the rolling circle mechanism of replication. IXth International Congress of Virology Glasgow: August, 1993. Abstract P60–18
    [Google Scholar]
  32. Surin B., Larkin P., Boevink P., Chu P., Keese P. 1993; The subterranean clover stunt virus genome consists of microchromosomes encoding single ORFs. IXth International Congress of Virology Glasgow: August, 1993. Abstract P62–1
    [Google Scholar]
  33. Thomas J. E., Dietzgen R. G. 1991; Purification, characterization and serological detection of virus-like particles associated with banana bunchy top disease in Australia. Journal of General Virology 72:217–224
    [Google Scholar]
  34. Upcroft P., Healey A. 1987; Rapid and efficient method for cloning of blunt-ended DNA fragments. Gene 51:69–75
    [Google Scholar]
  35. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
  36. Wu R. Y., Su H. J. 1990; Purification and characterization of banana bunchy top virus. Journal of Phytopathology 128:153–160
    [Google Scholar]
  37. Wu R. Y., You L. R., Soong T. S. 1994; Nucleotide sequences of two circular single-stranded DNAs associated with banana bunchy top virus. Phytopathology 84:952–958
    [Google Scholar]
  38. Yeh H., Su H., Chao Y. 1994; Genome characterization and identification of viral-associated dsDNA component of banana bunchy top virus. Virology 198:645–652
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-6-1471
Loading
/content/journal/jgv/10.1099/0022-1317-76-6-1471
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error