1887

Abstract

The RNA-2 of a carrot isolate from the English serotype of tomato black ring nepovirus (TBRV-ED) has been sequenced. It is 4618 nucleotides long and contains one open reading frame encoding a polypeptide of 1344 amino acids. The 5′ non-coding region contains three repetitions of a stem-loop structure also conserved in TBRV-Scottish and grapevine chrome mosaic nepovirus (GCMV). The coat protein domain was mapped to the carboxy-terminal one-third of the polyprotein. Sequence comparisons indicate that TBRV-ED RNA-2 probably arose by an RNA recombination event that resulted in the exchange of the putative movement protein gene between TBRV and GCMV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-5-1279
1995-05-01
2023-02-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/5/JV0760051279.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-5-1279&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment research tool. Journal of Molecular Biology 215:403–110
    [Google Scholar]
  2. Bacher J. W., Warkentin D., Ramsdell D., Hancock J. F. 1994; Selection versus recombination: what is maintaining identity in the 3′ termini of blueberry leaf mottle nepovirus RNA-1 and RNA-2?. Journal of General Virology 75:2133–2138
    [Google Scholar]
  3. Blok V. C., Wardell J., Jolly C. A., Manoukian A., Robinson D. J., Edwards M. L., Mayo M. A. 1992; The nucleotide sequence of RNA-2 of raspberry ringspot nepovirus. Journal of General Virology 73:2189–2194
    [Google Scholar]
  4. Brault V., Hibrand L., Candresse T., Le Gall O., Dunez J. 1989; Nucleotide sequence and genetic organization of Hungarian grapevine chrome mosaic nepovirus RNA2. Nucleic Acids Research 17:7809–7819
    [Google Scholar]
  5. Cervera M. T., Riechmann J. L., Martin M. T., Garcia J. A. 1993; 3′-Terminal sequence of the plum pox virus PS and 66 isolates: evidence for RNA recombination within the potyvirus group. Journal of General Virology 74:329–334
    [Google Scholar]
  6. Cyr D. M., Langer T., Douglas M. G. 1994; DnaJ-like proteins: molecular chaperones and specific regulators of Hsp70. Trends in Biochemical Sciences 19:176–181
    [Google Scholar]
  7. Demangeat G., Greif C., Hemmer O., Fritsch C. 1990; Analysis of the in vitro cleavage products of the tomato black ring virus RNA-1-encoded 250K polyprotein. Journal of General Virology 71:1649–1654
    [Google Scholar]
  8. Demangeat G., Hemmer O., Fritsch C., Le Gall O., Candresse T. 1991; In vitro processing of the RNA-2-encoded polyprotein of two nepoviruses: tomato black ring virus and grapevine chrome mosaic virus. Journal of General Virology 72:247–252
    [Google Scholar]
  9. Demangeat G., Hemmer O., Reinbolt J., Mayo M. A., Fritsch C. 1992; Virus-specific proteins in cells infected with tomato black ring nepovirus: evidence for proteolytic processing in vivo. Journal of General Virology 73:1609–1614
    [Google Scholar]
  10. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  11. Dodd S. M., Robinson D. J. 1987; Conservation of the 3-terminal sequences of the genome RNA species of tomato black ring virus. Journal of General Virology 68:973–978
    [Google Scholar]
  12. Doz B., Macquaire G., Delbos R., Dunez J. 1980; Caracteris-tiques et role du RNA3, RNA satellite du virus des anneaux noirs de la tomate. Annates de Virologie (Institut Pasteur) 131E:489–499
    [Google Scholar]
  13. Doz B., Delbos R., Dunez J. 1982; Premunition:une competition entre souches faibles et souches severes pour une voie commune depression des symptomes. La selection des plantes, Les colloques de FINRA, INRA Ed 11:29–44
    [Google Scholar]
  14. Fichot O., Girard M. 1990; An improved method for sequencing of RNA templates. Nucleic Acids Research 18:6162
    [Google Scholar]
  15. Fritsch C., Mayo M. A., Murant A. F. 1980; Translation products of genome and satellite RNAs of tomato black ring virus. Journal of General Virology 46:381–389
    [Google Scholar]
  16. Gibbs M. J., Cooper J. I. 1995; A recombinational event in the history of luteoviruses probably induced by base-pairing between the genomes of two distinct viruses. Virology (in press)
    [Google Scholar]
  17. Glaser P., Presecan E., Delepierre M., Surewicz W. K., Mantsch H. H., Barzu O., Gilles A. M. 1992; Zinc, a novel structural element found in the family of bacterial adenylate kinases. Biochemistry 31:3038–3043
    [Google Scholar]
  18. Greif C., Hemmer O., Fritsch C. 1988; Nucleotide sequence of tomato black ring virus RNA-1. Journal of General Virology 69:1517–1529
    [Google Scholar]
  19. Gubler U., Hoffman B. J. 1983; A simple and efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  20. Harrison B. D. 1958; Relationship between beet ringspot, potato bouquet and tomato black ring viruses. Journal of General Microbiology 18:450–460
    [Google Scholar]
  21. Harrison B. D., Murant A. F. 1977; Nematode transmissibility of pseudo-recombinant isolates of tomato black ring virus. Annals of Applied Biology 86:209–212
    [Google Scholar]
  22. Hibrand L., Le Gall O., Candresse T., Dunez J. 1992; Immunodetection of the proteins encoded by grapevine chrome mosaic nepovirus RNA-2. Journal of General Virology 73:2093–2098
    [Google Scholar]
  23. Higgins D. G., Bleasby A. J., Fuchs R. 1992; CLUSTAL V:improved software for multiple sequence alignment. Computer Applications in the Biosciences 8(2):189–191
    [Google Scholar]
  24. Hollings M., Stone O. M., Martelli G. P. 1969; Celery yellow vein and Hungarian chrome mosaic viruses. Reports of the Glasshouse Crops Research Institute 1968:102–103
    [Google Scholar]
  25. Koonin E. V., Mushegian A. R., Ryabov E. V., Dolja V. V. 1991; Diverse groups of plant RNA and DNA viruses share related movement proteins that may possess chaperone-like activity. Journal of General Virology 72:2895–2903
    [Google Scholar]
  26. Le Gall O., Candresse T., Brault V., Dunez J. 1989; Nucleotide sequence of Hungarian grapevine chrome mosaic nepovirus RNA1. Nucleic Acids Research 17:7795–7807
    [Google Scholar]
  27. LeGall O., Candresse T., Dunez J. 1995; Transfer of the 3′ non-translated region of grapevine chrome mosaic virus RNA-1 by recombination to tomato black ring virus RNA-2 in pseudorecombinant isolates. Journal of General Virology 76:1285–1289
    [Google Scholar]
  28. Lütcke H. A., Chow K. C., Mickel F. S., Moss K. A., Kern H. F., Scheele G. A. 1987; Selection of AUG initiation codons differs in plants and animals. EM BO Journal 6:43–18
    [Google Scholar]
  29. Martelli G. P., Quacquarelli A. 1972; Grapevine chrome mosaic virus. CMI/AAB Descriptions of Plant Viruses no 103
    [Google Scholar]
  30. Maxam A. M., Gilbert W. 1980; Sequencing end-labelled DNA with base-specific cleavages. Methods in Enzymology 65:499–559
    [Google Scholar]
  31. Mayo M. A., Martelli G. P. 1993; New families and genera of plant viruses. Archives of Virology 133:496–498
    [Google Scholar]
  32. Mayo M. A., Barker H., Harrison B. D. 1979; Polyadenylate in the RNA of five nepoviruses. Journal of General Virology 43:603–610
    [Google Scholar]
  33. Mayo M. A., Barker H., Harrison B. D. 1982; Specificity and properties of the genome-linked proteins of nepoviruses. Journal of General Virology 59:149–162
    [Google Scholar]
  34. Meyer M., Hemmer O., Mayo M. A., Fritsch C. 1986; The nucleotide sequence of tomato black ring virus RNA-2. Journal of General Virology 67:1257–1271
    [Google Scholar]
  35. Murant A. F. 1970; Tomato black ring virus. CMI/AAB Descriptions of Plant Viruses no 38
    [Google Scholar]
  36. Murant A. F. 1982; Tomato black ring virus (TBRV) in carrot. Reports of the Scottish Crop Research Institute 1981:114
    [Google Scholar]
  37. Murant A. F., Taylor M., Duncan G. H., Raschke J. H. 1981; Improved estimates of molecular weight of plant virus RNA by agarose gel electrophoresis and electron microscopy after denatu-ration with glyoxal. Journal of General Virology 41:53–61
    [Google Scholar]
  38. Mushegian A. R. 1994; The putative movement domain encoded by nepovirus RNA-2 is conserved in all sequenced nepoviruses. Archives of Virology 135:437–441
    [Google Scholar]
  39. Ohki M., Tamura F., Nishimura S., Uchida H. 1986; Nucleotide sequence of the Escherichia coli dnaJ gene and purification of the gene product. Journal of Biological Chemistry 261:1778–1781
    [Google Scholar]
  40. Ritzenthaler C., Michler P., Schmidt A. C., Stussi-Garaud C., PlNCK M., PlNCK L. 1994; In vitro and in vivo detection of grapevine fanleaf nepovirus P38 protein: involvement in tubule formation and viral movement in vivo.. EMBO-INRA Workshop ‘Plants and viruses: partners in pathogenicity’ Thiverval-Grignon; France: July 17–21 1994
    [Google Scholar]
  41. Robinson D. J., Barker H., Harrison B. D., Mayo M. A. 1980; Replication of RNA-1 of tomato black ring virus independently of RNA-2. Journal of General Virology 51:317–326
    [Google Scholar]
  42. Ron M. E., Tremaine J. H., Rochon D. M. 1991a; Nucleotide sequence of tomato ringspot virus RNA-2. Journal of General Virology 72:1505–1514
    [Google Scholar]
  43. Ron M. E., Tremaine J. H., Rochon D. M. 1991b; Comparison of the 5′ and 3 termini of tomato ringspot virus RNA-1 and RNA-2: evidence for RNA recombination. Virology 185:468–472
    [Google Scholar]
  44. Sawyer S. 1989; Statistical tests for detecting gene conversion. Molecular Biologv and Evolution 6:526–538
    [Google Scholar]
  45. Scott N. W., Cooper J. I., Liu Y. Y., Helen C. U. T. 1992; A 1.5 kb sequence homology in 3′-terminal regions of RNA-1 and RNA-2 of a birch isolate of cherry leaf roll nepovirus is also present, at least in part, in a rhubarb isolate. Journal of General Virology 73:481–485
    [Google Scholar]
  46. Serghini M. A., Fuchs M., Pinck M., Reinbolt J., Walter B., Pinck L. 1990; RNA-2 of grapevine fanleaf virus: sequence analysis and coat protein cistron location. Journal of General Virology 71:1433–1441
    [Google Scholar]
  47. Steinkellner H., Himmler G., Sagl R., Mattanovich D., Katinger H. 1992; Amino-acid sequence comparison of nepovirus coat proteins. Virus Genes 6:197–202
    [Google Scholar]
  48. Wieczorek A., Sanfaҫon H. 1993; Characterization and subcellular localization of tomato ringspot nepovirus putative movement protein. Virology 194:734–742
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-5-1279
Loading
/content/journal/jgv/10.1099/0022-1317-76-5-1279
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error