1887

Abstract

Cassava vein mosaic virus (CVMV) was found to be widespread throughout the north-eastern region of Brazil. The complete sequence of CVMV was determined, and the genome was 8158 bp in size. A cytosolic initiator methionine tRNA (tRNA )-binding site that probably acts as a primer for minus-strand synthesis was present. The genome contained five open reading frames that potentially encode proteins with predicted molecular masses of 186 kDa, 9 kDa, 77 kDa, 24 kDa and 26 kDa. The putative 186 kDa protein had regions with similarity to the zinc finger-like RNA-binding domain that is a common element in the capsid proteins and similarity to the intercellular transport domain of the plant pararetroviruses. The predicted 77 kDa protein had regions with similarity to aspartic proteases, reverse transcriptase and RNase H of pararetroviruses. This gene order was confirmed by the amplification of similar PCR products from total DNA extracted from CVMV-infected cassava plants. The genomic organization of CVMV was different from the organization of either the caulimoviruses or badnaviruses. In comparisons of the regions with the reverse transcriptase motif, CVMV was grouped between the caulimoviruses and badnaviruses. It appears that CVMV is distinct from the other well-characterized plant pararetroviruses.

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1995-05-01
2021-09-23
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References

  1. Anza O. A. 1982 Replication and mapping of caulimoviruses PhD thesis University of California-Davis, USA:
    [Google Scholar]
  2. Bouhida M., Lockhart B. E. L., Olszewski N. E. 1993; An analysis of the complete sequence of a sugarcane bacilliform virus genome infectious to banana and rice. Journal of General Virology 74:15–22
    [Google Scholar]
  3. Covey S. N. 1986; Amino acid sequence homology in gag region of reverse transcribing elements and the coat protein of cauliflower mosaic virus. Nucleic Acids Research 14:623–633
    [Google Scholar]
  4. Dellaporta S. L., Wood J., Hicks J. B. 1983; A plant DNA minipreparation: version II. Plant Molecular Biology Reporter 1:19–21
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phytogenies: an approach using the bootstrap. Evolution 39:783–791
    [Google Scholar]
  6. Gardner R. C., Shepherd R. J. 1980; A procedure for rapid isolation and analysis of cauliflower mosaic virus DNA. Virology 106:159–161
    [Google Scholar]
  7. Gardner R. C., Howarth A. J., Hahn P., Brown-Luedi M., Shepherd R. J., Messing J. 1981; The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by M13mp7 shotgun sequencing. Nucleic Acids Research 9:2871–2888
    [Google Scholar]
  8. Guilfoyle T. J. 1987; Retrolike viruses in plants. In PlantMicrobe Interactions:Molecular and Genetic Perspectives pp 327–358 Edited by Kosuge T., Nester E. W. New York: Macmillan Publishing;
    [Google Scholar]
  9. Hasagawa A., Verver J., Shimada A., Saito M., Goldbach R., Van Kammen A., Miki K., Kameya-Iwaki M., Hibi T. 1989; The complete sequence of soybean chlorotic mottle virus DNA and the identification of a novel promoter. Nucleic Acids Research 17:9993–10012
    [Google Scholar]
  10. Hay J. M., Jones M. C., Blakebrough M. L., Dasgupta L., Davies J. W., Hull R. 1991; An analysis of the sequence of an infectious clone of rice tungro bacilliform virus, a plant pararetrovirus. Nucleic Acids Research 19:2615–2621
    [Google Scholar]
  11. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
    [Google Scholar]
  12. Hull R. 1984; Caulimovirus group. CMI/ABB Descriptions of Plant Viruses no 299
    [Google Scholar]
  13. Hull R., Sadler J., Longstaff M. 1986; The sequence of carnation etched ring virus DNA: comparison with cauliflower mosaic virus and retroviruses. EMBO Journal 5:3083–3090
    [Google Scholar]
  14. Hull R., Covey S. N., Maule A. J. 1987; Structure and replication of caulimovirus genomes. Journal of Cell Science (Suppl.) 7213–229
    [Google Scholar]
  15. Kitajima E. W., Costa A. S. 1966; Particulas esferoidais associadas ao virus do mosaico das nervuras da mandioca. Bragantia 25:211–221
    [Google Scholar]
  16. Koonin E. V., Mushegian A. R., Ryabov E. V., Dolja V. V. 1991; Diverse groups of plant RNA and DNA viruses share related movement proteins that may possess chaperone-like activity. Journal of General Virology 72:2895–2903
    [Google Scholar]
  17. Lin M. T., Kitajima E. W. 1980; Purifigao e serologia do virus do mosaico das nervuras da mandioca. Fitopatologia Brasilera 5:419 (abstract)
    [Google Scholar]
  18. Lockhart B. E. L. 1990; Evidence for a double-stranded circular DNA genome in a second group of plant viruses. Phytopathology 80:127–131
    [Google Scholar]
  19. Mason W. S., Taylor J. M., Hull R. 1987; Retroid virus genome replication. Advances in Virus Research 32:35–96
    [Google Scholar]
  20. Medberry S. L., Lockhart B. E. L., Olszewski N. E. 1990; Properties of Commelina yellow mottle virus’s complete DNA sequence, genomic discontinuities and transcript suggest that it is a pararetrovirus. Nucleic Acids Research 18:5505–5513
    [Google Scholar]
  21. Qu R., Bhattacharyya M., Laco G. S., De Kochko A., Rao B. L. S., Kaniewska M. B., Elmer J. S., Rochester D. E., Smith C. E., Beachy R. N. 1991; Characterization of the genome of rice tungro bacilliform virus: comparison with Commelina yellow mottle virus and caulimoviruses. Virology 185:354–364
    [Google Scholar]
  22. Richins R. D., Scholthof H. B., Shepherd R. J. 1987; Sequence of figwort mosaic virus DNA (caulimovirus group). Nucleic Acids Research 15:8451–8466
    [Google Scholar]
  23. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  24. Sprinzl M., Hartman T., Meissner F., Moll J., Vorder-Wulbecke T. 1987; Compilation of tRNA sequences and sequences of tRNA genes. Nucleic Acids Research (Suppl.) 15r531–rl88
    [Google Scholar]
  25. Temin H. M. 1989; Retrons in bacteria. Nature 339:254–255
    [Google Scholar]
  26. Toh H., Hayashida H., Miyata T. 1983; Sequence homology between retroviral reverse transcriptase and putative polymerases of hepatitus virus and cauliflower mosaic virus. Nature 305:827–829
    [Google Scholar]
  27. Vieira J., Messing J. 1987; Production of single-stranded plasmid DNA. Methods in Enzvmology 153:3–11
    [Google Scholar]
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