Comparison of bovine coronavirus isolates associated with neonatal calf diarrhoea and winter dysentery in adult dairy cattle in Québec Free

Abstract

Cytopathic coronaviruses were isolated in HRT-18 cells from bloody faecal samples collected from cows in Québec dairy herds having experienced typical outbreaks of winter dysentery (WD). The formation of polykaryons in the infected cell cultures was found to be dependent on the presence of trypsin in the medium. The WD isolates differed from the prototype Mebus strain of bovine enteropathogenic coronavirus (BCV.Meb) in respect to haemagglutination inhibition (HI), haemagglutination patterns at 4 °C and 37 °C, and receptor destroying enzyme activity with rat erythrocytes. Other field strains of BCV associated with outbreaks of neonatal calf diarrhoea (NCD) also differed from the BCV.Meb strain by demonstrating differences in HI. In all cases, no differences were detected by virus neutralization and Western immunoblotting. Analysis and comparison of the nucleotide and deduced amino acid sequences of the PCR-amplified haemagglutinin esterase (HE) genes of one representative WD strain (BCQ.2590) and two highly cytopathic NCD strains (BCQ.3 and BCQ.571) revealed high degrees of similarities (nt and aa sequence homologies > 98%) with the BCV.Meb strain. The putative esterase active site FGDS was conserved among these four BCV strains, indicating that this domain is probably not a determinant for BCV virulence. Six amino acid substitutions occurred between the HE glycoproteins of BCV.Meb and BCQ.2590 strains; two proline substitutions occurred respectively in the signal peptide (at aa 5) and near the sequences of the putative esterase domain (at aa 53).

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1995-05-01
2024-03-28
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References

  1. Abraham S., Kienzle T. E., Lapps W., Brian D. A. 1991; Deduced sequence of the bovine coronavirus spike protein and identification of the internal proteolytic cleavage site. Virologv 176:296–301
    [Google Scholar]
  2. Athanassious R., Marsolais G., Assaf R., Dea S., Descoteaux J. P., Dulude S., Montpetit C. 1994; Detection of bovine coronavirus and type A rotavirus in neonatal calf diarrhea and winter dysentery of cattle in Québec: evaluation of three diagnostic methods. Canadian Veterinary Journal 35:163–169
    [Google Scholar]
  3. Benfield D. A., Saif L. J. 1990; Cell culture propagation of a coronavirus isolated from cows with winter dysentery. Journal of Clinical Microbiology 28:1454–1457
    [Google Scholar]
  4. Campbell S. G., Cookingham C. A. 1978; The enigma of winter dysentery. Cornell Veterinarian 68:423–441
    [Google Scholar]
  5. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform. Analytical Biochemistry 162:156–159
    [Google Scholar]
  6. Dea S., Tijssen P. 1989; Antigenic and polypeptide structure of turkey enteric coronaviruses as defined by monoclonal antibodies. Journal of General Virology 70:1725–1741
    [Google Scholar]
  7. Dea S., Garzon S. 1991; Identification of coronaviruses by the use of indirect protein-A immunogold electron microscopy. Journal of Veterinary Diagnostic Investigation 3:297–305
    [Google Scholar]
  8. Dea S., Roy R. S., Begin M. E. 1980; Bovine coronavirus isolation in continuous cell lines. American Journal of Veterinary Research 41:30–38
    [Google Scholar]
  9. Dea S., Garzon S., Tijssen P. 1989; Isolation and trypsin-enhanced propagation of turkey enteric (Bluecomb) coronaviruses in a continuous HRT-18 cell line. American Journal of Veterinarv Research 50:1310–1318
    [Google Scholar]
  10. Deregt D., Sabara M., Babiuk L. A. 1988; Structural proteins of bovine coronavirus and their intracellular processing. Journal of General Virology 68:2863–2877
    [Google Scholar]
  11. Durham P. J. K., Hassard L. E., Armstrong K. R., Naylor J. M. 1989; Coronavirus-associated diarrhea (winter dysentery) in adult cattle. Canadian Veterinary Journal 30:825–827
    [Google Scholar]
  12. Espinasse J., Viso M., Laval A., Savey M., Le Layec C. 1982; Winter dysentery: a coronavirus-like agent in the feces of beef and dairy cattle with diarrhoea. Veterinary Record 110:385
    [Google Scholar]
  13. King B., Potts B. J., Brian D. A. 1985; Bovine coronavirus hemagglutinin protein. Virus Research 2:53–59
    [Google Scholar]
  14. Mebus C. A., Stair E. L., Rhodes M. B., Twiehaus M. J. 1973; Neonatal calf diarrhoea: propagation, attenuation, and characteristics of a coronavirus-like agent. American Journal of Veterinary Research 34:145–150
    [Google Scholar]
  15. Michaud L., Dea S. 1993; Characterization of monoclonal antibodies to bovine enteric coronavirus and antigenic variations among Québec isolates. Archives of Virology 131:455–465
    [Google Scholar]
  16. Parker M. D., Cox G. J., Deregt D., Fitzpatrick D. R., Babiuk L. A. 1989; Cloning and in vitro expression of the gene for the E3 haemagglutinin glycoprotein of bovine coronavirus. Journal of General Virology 70:155–164
    [Google Scholar]
  17. Parker M. D., Yoo D., Cox G. J., Babiuk L. A. 1990; Primary structure of the S peplomer gene of bovine coronavirus and surface expression in insect cells. Journal of General Virology 71:263–270
    [Google Scholar]
  18. Rekik M. R., Dea S. 1994; Comparative sequence analysis of a polymorphic region of spike protein gene of bovine coronavirus isolates. Archives of Virology 135:319–331
    [Google Scholar]
  19. Reynolds D. J., Debney T. G., Hall G. A., Thomas L. H., Parsons K. R. 1985; Studies on the relationship between coronaviruses from the intestinal and respiratory tracts of calves. Archives of Virology 85:71–83
    [Google Scholar]
  20. Saif L. J., Redman D. R., Brock K. V., Kohler E. M., Heckerr R. A. 1988; Winter dysentery in adult dairy cattle: detection of coronaviruses in the feces. Veterinary Record 123:300–301
    [Google Scholar]
  21. Saif L. J., Brock K. V., Redman D. R., Kohler E. M. 1991; Winter dysentery in dairy herds: electron microscopic and serological evidence for an association with coronavirus infection. Veterinary Record 128:447–449
    [Google Scholar]
  22. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  23. Schultze B., Wahn K., Klenk H. D., Herrler G. 1991a; Isolated HE-protein from hemagglutinating encephalomyelitis virus and bovine coronavirus has receptor-binding activity. Virology 180:221–228
    [Google Scholar]
  24. Schultze B., Gross H. J., Brossmer R., Herrler G. 1991b; The S protein of bovine coronavirus is a hemagglutinin recognizing 9-O-acetylated sialic acid as a receptor determinant. Journal of Virology 65:6232–6237
    [Google Scholar]
  25. Spaan W., Cavanagh D., Horzinek M. C. 1988; Corona viruses: structure and genome expression. Journal of General Virology 69:2939–2952
    [Google Scholar]
  26. Storz J., Herrler G., Snodgrass D. R., Hussain K. A., Zhang X. M., Clark M. A., Rott R. 1991; Monoclonal antibodies differentiate between the haemagglutinating and the receptor-destroying activities of bovine corona virus. Journal of General Virology 72:2817–2820
    [Google Scholar]
  27. Storz J., Zhang X. M., Rott R. 1992; Comparison of hemagglutinating, receptor-destroying, and acetylesterase activities of avirulent and virulent bovine corona virus strains. Archives of Virology 125:193–204
    [Google Scholar]
  28. Takahashi E., Inaba Y., Sato K., Ito Y., Kurogi H., Akashi H., Satoda K., Omori T. 1980; Epizootic diarrhoea of adult cattle associated with a coronavirus-like agent. Veterinary Microbiology 5:151–154
    [Google Scholar]
  29. Vautherot J.-F., Madelaine M.-F., Boireau P., Laporte J. 1992; Bovine corona virus peplomer glycoproteins: detailed antigenic analyses of S1, S2 and HE. Journal of General Virology 73:1725–1737
    [Google Scholar]
  30. Vlasak R., Luytjes W., Leider J., Spaan W., Palese P. 1988; The E3 protein of bovine corona virus is a receptor-destroying enzyme with acetylesterase activity. Journal of Virology 62:46864690
    [Google Scholar]
  31. Yaron A., Naider F. 1993; Proline-dependent structural and biological properties of peptide and proteins. Critical Reviews in Biochemistry and Molecular Biology 28:31–80
    [Google Scholar]
  32. Zhang X., Kousoulas K. G., Storz J. 1991; The hemagglutinin/ esterase glycoprotein of bovine corona virus: sequence and functional comparisons between virulent and avirulent strains. Virology 185:847–852
    [Google Scholar]
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