1887

Abstract

COS-7 cells transfected with parvovirus B19-simian virus 40 (SV40) hybrid vectors have previously been shown to express B19 structural proteins. In this study the morphology and antigenicity of B19 proteins expressed in these cells were investigated. At 84 h after transfection, approximately 10% of the COS-7 cells expressed B19 antigen, and the yield was equivalent to 2 × 10 to 2 × 10 B19 particles/transfected cell. The B19 proteins self-assembled into capsids that were morphologically and antigenically similar to native B19 virions, and could substitute for native antigen in a B19 IgM assay. Recombinant capsids lacking the recently described 11 kDa protein also resembled native virions.

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1995-05-01
2022-05-26
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References

  1. Anderson M. J. 1987; Parvoviruses as agents of human disease. Progress in Medical Virology 34:55–69
    [Google Scholar]
  2. Anderson L. J., Tsou C., Parker R. A., Chorba T. L., Wulff H., Tattersall P., Mortimer P. P. 1986; Detection of antibodies and antigens of human parvovirus B19 by enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 24:522526
    [Google Scholar]
  3. Beard C., St Amand J., Astell C. R. 1989; Transient expression of B19 parvovirus gene products in COS-7 transfected with B19-SV40 hybrid vectors. Virology 172:659–664
    [Google Scholar]
  4. Brown K. E., Cohen B. J. 1992; Haemagglutination by parvovirus B19. Journal of General Virology 73:2147–2149
    [Google Scholar]
  5. Brown C. S., Van Bussel M. J., Wassenaar A. L., van Elsacker-Niele A. M., Weiland H. T., Salimans M. M. 1990; An immunofluorescence assay for the detection of parvovirus B19 IgG and IgM antibodies based on recombinant viral antigen. Journal of Virological Methods 29:53–62
    [Google Scholar]
  6. Brown C. S., Van Lent J. W., Vlak J. M., Spaan W. J. 1991; Assembly of capsids by using baculovirus recombinants expressing human parvovirus B19 structural proteins. Journal of Virology 65:2702–2706
    [Google Scholar]
  7. Brown C. S., Jenson T., Meloen R. H., Puijk W., Sugamura K., Sato H., Spaan W. J. M. 1992; Localization of an immunodominant domain on baculovirus-produced B19 capsids: correlation to a major surface region on the native virus particle. Journal of Virology 66:6989–6996
    [Google Scholar]
  8. Brown K. E., Anderson S. M., Young N. S. 1993; Erythrocyte P antigen: cellular receptor for B19 parvovirus. Science 262:114–117
    [Google Scholar]
  9. Cohen B. J., Mortimer P. P., Pereira M. S. 1983; Diagnostic assays with monoclonal antibodies for the human serum parvoviruslike virus (SPLV). Journal of Hygiene 91:113–130
    [Google Scholar]
  10. Cohen B. J., Courouce A. M., Schwarz T. F., Okochi K., Kurtzman G. J. 1988; Laboratory infection with parvovirus B19 [letter]. Journal of Clinical Pathology 41:1027–1028
    [Google Scholar]
  11. Cohen B. J., Field A. M., Gudnadottir S., Beard S., Barbara J. A. 1990; Blood donor screening for parvovirus B19. Journal of Virological Methods 30:233–238
    [Google Scholar]
  12. Fridell E., Cohen B. J., Wahren B. 1991; Evaluation of a synthetic-peptide enzyme-linked immunosorbent assay for immunoglobulin M to human parvovirus B19. Journal of Clinical Microbiology 29:1376–1381
    [Google Scholar]
  13. Kajigaya S., Shimada T., Fujita S., Young N. S. 1989; A genetically engineered cell line that produces empty capsids of B19 (human) parvovirus. Proceedings of the National Academy of Sciences, USA 86:7601–7605
    [Google Scholar]
  14. Kajigaya S., Fujii H., Field A., Anderson S., Rosenfield S., Anderson L. J., Shimada T., Young N. S. 1991; Self-assembled B19 parvovirus capsids, produced in a baculovirus system, are antigenically and immunogenically similar to native virions. Proceedings of the National Academy of Sciences, USA 88:4646–4650
    [Google Scholar]
  15. Koch W. C., Adler S. P. 1989; Human parvovirus B19 infections in women of childbearing age and within families. Pediatric Infectious Diseases Journal 8:83–87
    [Google Scholar]
  16. Luo W., Astell C. R. 1993; A novel protein encoded by small RNAs of parvovirus B19. Virology 195:448–455
    [Google Scholar]
  17. Mori J., Field A. M., Clewley J. P., Cohen B. J. 1989; Dot blot hybridisation assay of B19 virus DNA in clinical specimens. Journal of Clinical Microbiology 27:459–464
    [Google Scholar]
  18. O’Neill H. J., Coyle P. V. 1992; Two anti-parvovirus B19 IgM capture assays incorporating a mouse monoclonal antibody specific for B19 viral capsid proteins VP1 and VP2. Archives of Virology 123:125–134
    [Google Scholar]
  19. Rayment F. B., Crosdale E., Morris D. J., Pattison J. R., Talbot P., Clare J. J. 1990; The production of human parvovirus capsid proteins in Escherichia coli and their potential as diagnostic antigens. Journal of General Virology 71:2665–2672
    [Google Scholar]
  20. Rosenfeld S. J., Young N. S., Alling D., Ayub J., Saxinger C. 1994; Subunit interaction in B19 empty capsids. Archives of Virology 136:9–18
    [Google Scholar]
  21. Salimans M. M. M., van Bussel J. A. W. M., Brown C. S., Spaan W. J. M. 1992; Recombinant parvovirus B19 capsids as a new substrate for detection of B 19-specific IgG and IgM antibodies by an enzyme-linked immunosorbent assay. Journal of Virological Methods 39:247–258
    [Google Scholar]
  22. Sato H., Hirata J., Furukawa M., Kuroda N., Shiraki H., Maeda Y., Okochi K. 1991; Identification of the region including the epitope for a monoclonal antibody which can neutralize human parvovirus B19. Journal of Virology 65:1667–1672
    [Google Scholar]
  23. Sisk W. P., Berman M. L. 1987; Expression of human parvovirus B19 structural protein in E. coli and detection of anti-viral antibodies in human serum. Bio/Technology 5:1077–1080
    [Google Scholar]
  24. St Amand & Astell C. R. 1993; Identification and characterization of a family of 11-kDa proteins encoded by the human parvovirus B19. Virology 192:121–131
    [Google Scholar]
  25. Yoshimoto K., Rosenfeld S., Frickhofen N., Kennedy D., Hills R., Kajigaya S., Young N. S. 1991; A second neutralizing epitope of B19 parvovirus implicates the spike region in the immune response. Journal of Virology 65:7056–7060
    [Google Scholar]
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