1887

Journal of General Virology header logo

Volume 76, Issue 5

Human papillomavirus type 16 capsids expose multiple type-restricted and type-common antigenic epitopes Free

Abstract

The study of viral infectivity and detection of viral capsid antigens of the major cervical cancer-associated human papillomavirus (HPV) type, HPV-16, requires knowledge of which epitopes are exposed in clinical specimens of infected tissue or on intact capsids. To define the antigenic epitopes of HPV-16, antisera to 66 overlapping synthetic peptides corresponding to the HPV-16 capsid proteins L1 and L2 and to seven peptide analogues were tested in immunoperoxidase stainings of consecutive sections from formalin-fixed, paraffin-embedded HPV infected tissue. Antisera against eleven different peptides from L1 and against seven different peptides from L2 recognized the HPV capsid antigen. Most epitopes were only found on the capsid antigen of certain genital HPV types, but four antigenic epitopes in L1 were detectable also in cutaneous wart specimens. All antigenic epitopes in L2 were restricted to genital HPV types and four L2 epitopes were only detectable in HPV-16 or HPV-33 positive specimens. The surface exposure of the antigenic epitopes was investigated by comparing the reactivity of the antipeptide antisera with intact or disrupted virions or capsids of HPV-11, HPV-16 and bovine papillomavirus (BPV). Twenty antipeptide sera from L1 and seven antipeptide sera from L2 were reactive with intact HPV-16 capsids at titres up to 1:146000. Sixteen of these antisera were also reactive with disrupted HPV-16 capsids. Cross-reactivity with disrupted HPV-11 and BPV was detected for eleven and six antisera, respectively, whereas intact HPV-11 or BPV virions showed only weak cross-reactivity. In conclusion, the HPV-16 L1 and L2 capsid proteins contained multiple antigenic epitopes, most of which were shared with one or several additional HPV types.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1995
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-5-1141
1995-05-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/5/JV0760051141.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-5-1141&mimeType=html&fmt=ahah

References

  1. Baker T. S., Newcomb W. W., Olson N. H., Cowsert L. M., Olson C., Brown J. C. 1991; Structures of bovine and human papillomaviruses. Analysis by cryoelectron microscopy and three–dimensional image reconstruction. Biophysical Journal 60:1445–1456
     [Google Scholar]
  2. Barrera-Oro J. G., Smith K. O., Melnick J. L. 1962; Quantitation of papovavirus particles in human warts. Journal of the National Cancer Institute 29:583–595
     [Google Scholar]
  3. Bistoletti P., Zellbi A., Moreno-Lopez J., Hjerpe A. 1988; Genital papillomavirus infection after treatment for cervical intraepithelial neoplasia (CIN). Cancer 62:2056–2059
     [Google Scholar]
  4. Cason J., Kambo P. K., Jewers R. J., Chrystie I. L., Best J. M. 1993; Mapping of linear B cell epitopes on capsid proteins of bovine papillomavirus: identification of three external type-restricted epitopes. Journal of General Virology 74:2669–2677
     [Google Scholar]
  5. Cason J., Patel D., Naylor J., Lunney D., Shepherd P. S., Best J. M., McCance D. J. 1989; Identification of immunogenic regions of the major coat protein of human papillomavirus type 16 that contain type-restricted epitopes. Journal of General Virology 70:2973–2987
     [Google Scholar]
  6. Christensen N. D., Kreider J. W. 1991; Neutralization of CRPV infectivity by monoclonal antibodies that identify conformational epitopes on intact virions. Virus Research 21:169–179
     [Google Scholar]
  7. Christensen N. D., Kreider J. W. 1993; Monoclonal antibody neutralization of BPV-1. Virus Research 28:195–202
     [Google Scholar]
  8. Christensen N. D., Kreider J. W., Cladel N. M., Galloway D. A. 1990a; Immunological cross-reactivity to laboratory-produced HPV 11 virions of polysera raised against bacterially derived fusion proteins and synthetic peptides of HPV-6b and HPV-16 capsid proteins. Virology 175:1–9
     [Google Scholar]
  9. Christensen N. D., Kreider J. W., Cladel N. M., Patrick S. D., Welsh P. A. 1990b; Monoclonal antibody-mediated neutralization of infectious human papillomavirus type 11. Journal of Virology 64:5678–5681
     [Google Scholar]
  10. Christensen N. D., Kreider J. K., Kan N. C., DiAngelo S. L. 1991; The open reading frame L2 of cottontail rabbit papillomavirus contains antibody-inducing neutralizing epitopes. Virology 181:572–579
     [Google Scholar]
  11. Christensen N. D., Kreider J. W., Shah K. V., Rando R. F. 1992; Detection of human serum antibodies that neutralize infectious human papillomavirus type 11 virions. Journal of General Virology 73:1261–1267
     [Google Scholar]
  12. Cowsert F. M., Lake P., Jenson A. B. 1987; Topographical and conformational epitopes of bovine papillomavirus type 1 defined by monoclonal antibodies. Journal of the National Cancer Institute 79:1053–1057
     [Google Scholar]
  13. De Villiers E. M. 1992; Laboratory techniques in the investigation of human papillomavirus infection. Genitourinary Medicine 68:50–54
     [Google Scholar]
  14. Dillner J., Dillner L., Utter G., Eklund C., Rotola A., Costa S., Diluca D. 1990; Mapping of linear epitopes of human papillomavirus type 16: the L1 and L2 open reading frames. International Journal of Cancer 45:529–535
     [Google Scholar]
  15. Dillner J., Lenner P., Lehtinen M., Eklund C., Heino P., Wiklund F., Hallmans G., Stendahl U. 1994; A population-based seroepidemiological study of cervical cancer. Cancer Research 54:134–141
     [Google Scholar]
  16. Dillner L., Heino P., Moreno-Lopez J., Dillner J. 1991; Antigenic and immunogenic epitopes shared by human papillomavirus type 16 and bovine, canine, and avian papillomaviruses. Journal of Virology 65:6862–6871
     [Google Scholar]
  17. Favre M., Breitburd F. O. C., Orth G. 1975; Structural polypeptides of rabbit, bovine, and human papillomaviruses. Journal of Virology 15:1239–1247
     [Google Scholar]
  18. Ghim S.-J., Jenson B., Schlegel R. 1992; HPV-1 L1 protein expressed in COS cells displays conformational epitopes found on intact virions. Virology 190:548–552
     [Google Scholar]
  19. Jin X. W., Cowsert L. M., Pilacinski W. P., Jenson A. B. 1989; Identification of L2 open reading frame gene products of bovine papillomavirus type 1 using monoclonal antibodies. Journal of General Virology 63:1133–1140
     [Google Scholar]
  20. Kirnbauer R., Booy F., Cheng N., Lowy D. R., Schiller A. T. 1992; Papillomavirus L1 major capsid protein self-assembles into virus-like particles that are highly immunogenic. Proceedings of the National Academy of Sciences, USA 89:12180–12184
     [Google Scholar]
  21. Kirnbauer R., Taub J., Greenstone H., Roden R., Durst M., Gissman L., Lowy D. R., Schiller J. T. 1993; Efficient selfassembly of human papillomavirus type 16 L1 and L1-L2 into viruslike particles. Journal of Virology 67:6929–6936
     [Google Scholar]
  22. Komly C. A., Breitburd F., Croissant O., Streeck R. E. 1986; The L2 open reading frame of human papillomavirus type la encodes a minor structural protein carrying type-specific antigens. Journal of Virology 60:813–816
     [Google Scholar]
  23. Kreider J. W., Howett M. K., Wolfe S. A., Barlett G. L., Zaino R. J., Sedlacek T. V., Mortel R. 1985; Morphological transformation in vivo of human uterine cervix with papillomavirus from condyloma acuminata. Nature 317:639–640
     [Google Scholar]
  24. Lehtinen M., Niemela J., Dillner J., Parkkonen P., Nummi T., Liski E., Nieminen P., Reunala T., Paavonen J. 1993; Evaluation of serum antibody response to newly identified B-cell epitope in the minor nucleocapsid protein L2 of human papillomavirus type 16. Clinical and Diagnostic Virology 1:153–165
     [Google Scholar]
  25. Li C. C. H., Shah K. V., Seth A., Gilden R. V. 1987; Identification of the human papillomavirus type 6b L1 open reading frame protein in condylomas and corresponding antibodies in human sera. Journal of Virology 61:2684–2690
     [Google Scholar]
  26. Lorincz A. T., Reid R., Jenson A. B., Greenberg M. D., Lancaster W. D., Kurman R. J. 1992; Human papillomavirus infection of the cervix: relative risk associations of 15 common anogenital types. Obstetrics and Gynecology 79:328–337
     [Google Scholar]
  27. Patel D., Shepherd P. S., Naylor J. A., McCance D. J. 1989; Reactivities of polyclonal and monoclonal antibodies raised to the major capsid protein of human papillomavirus type 16. Journal of General Virology 70:69–77
     [Google Scholar]
  28. Pfister H. 1987; Human papillomaviruses and genital cancer. Advances in Cancer Research 48:113–147
     [Google Scholar]
  29. Pilacinski W. P., Glassman D. L., Krzyzek R. A., Sadowski A. K. 1984; Cloning and expression in E. coli of the bovine papillomavirus L1 and L2 open reading frames. Biotechnology 2:356–360
     [Google Scholar]
  30. Skyldberg B., Hagmar B., Johansson B., Kalantari M., Moberger B., Walaas L., Warleby B. 1995; HPV detection in cylogical cases with condylomatous or dysplastic changes. A study with PCR and in situ hybridizaton on cytological material. Diagnostic Cytopathology
     [Google Scholar]
  31. Tomita Y., Shirasawa H., Simizu B. 1987; Expression of human papillomavirus type 6b and 16 L1 open reading frames in Escherichia coli: detection of a 56000 dalton polypeptide containing genus specific (common), antigens. Journal of Virology 61:2389–2394
     [Google Scholar]
  32. Volpers C., Sapp M., Komly C. A., Richalet-Secordel P., Streeck R. E. 1993; Development of type-specific and crossreactive serological probes for the minor capsid protein of human papillomavirus type 33. Journal of Virology 67:1927–1935
     [Google Scholar]
  33. Yaegashi N., Jenison S. A., Batra M., Galloway D. A. 1992; Human antibodies recognize multiple distinct type-specific and cross-reactive regions of the minor capsid proteins of human papillomavirus types 6 and 11. Journal of Virology 66:2008–2019
     [Google Scholar]
  34. zur Hausen H. 1991; Viruses in human cancers. Science 254:1167–1173
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-5-1141
Loading
Human papillomavirus type 16 capsids expose multiple type-restricted and type-common antigenic epitopes
J. Gen. Virol. 76, 1141 (1995); https://doi.org/10.1099/0022-1317-76-5-1141
/content/journal/jgv/10.1099/0022-1317-76-5-1141
/content/journal/jgv/10.1099/0022-1317-76-5-1141
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error