1887

Abstract

Complete sequence data for the RNA 3 of both citrus leaf rugose (CiLRV) and citrus variegation (CVV) ilarviruses have been determined. The RNAs are 2289 nt (CiLRV) and 2309 nt (CVV) in length and both contain the typical arrangement of two open reading frames (ORFs) which, when translated, code for proteins that correspond to the 32 000 (32K) putative movement proteins (ORF 1) and the coat proteins (ORF 2) of the respective viruses. The 3′ termini of both viruses can be folded to form a secondary structure similar to those reported for other ilarviruses. These are the first complete nucleotide sequences for RNA 3 of members of subgroup 2 of the ilarviruses. The two viruses share substantial homology in nucleic acid sequence, code for identically sized coat proteins and share high levels of identity in the translated products of both ORFs. Although related, these viruses differ sufficiently to be considered distinct. The RNA 3s of CiLRV and CVV appear to be distinct from those of other ilarviruses for which comparable sequence data are available and also from the closely related alfalfa mosaic virus.

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1995-04-01
2021-10-28
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References

  1. Alrefai R. H., Shiel P. J., Domier L. L., D’Arcy C. J., Berger P. H., Korban S. S. 1994; The nucleotide sequence of apple mosaic virus coat protein gene has no homology with other Bromoviridae coat protein genes. Journal of General Virology 75:2847–2850
    [Google Scholar]
  2. Bachman E. J., Scott S. W., Xin Ge & Vance V. B. 1994; The complete nucleotide sequence of prune dwarf ilarvirus RNA3: implications for coat protein activation of genome replication in ilarviruses. Virology 201:127–131
    [Google Scholar]
  3. Barker R. F., Jarvis N. P., Thompson D. V., Loesch–Fries L. S., Hall T. C. 1983; Complete nucleotide sequence of alfalfa mosaic virus RNA 3. Nucleic Acids Research 11:2881–2891
    [Google Scholar]
  4. Cornelissen B. J. C., JANSSEN H. K., Zuidema D., BOL J. F. 1984; Complete nucleotide sequence of tobacco streak virus RNA 3. Nucleic Acids Research 12:2427–2437
    [Google Scholar]
  5. De Bellis G., Manoni M., Pergolizzi M. E., Redolfi M. E., Luzzana M. 1992; A more stringent choice of primers can improve the performance of fluorescent automated DNA sequencers. BioTechniques 13:892–897
    [Google Scholar]
  6. DeBorde D. C., Naeve C. W., Herlocher M. L., Massab H. F. 1986; Resolution of a common RNA sequencing ambiguity by terminal deoxynucleotidyl transferase. Analytical Biochemistry 157:275–287
    [Google Scholar]
  7. Francki R. I. B., Fauquet C. M., Knudson D. L., Brown F. 1991; Classification and Nomenclature of Viruses. Fifth Report of the International Committee on Taxonomy of Viruses. Archives of Virology Supplementum 2:389–391
    [Google Scholar]
  8. Garnsey S. M. 1975; Purification and properties of citrus leaf rugose virus. Phytopathology 65:50–57
    [Google Scholar]
  9. Ge X., Scott S. W. 1994; The nucleotide sequence of citrus leaf rugose ilarvirus RNA-2. Journal of General Virology 75:2841–2846
    [Google Scholar]
  10. Houser-Scott F., Baer M. L., Liem K. F., Cai J., Gehrke L. 1994; Nucleotide sequence and structural determinants of specific binding of coat protein or coat protein peptides to the 3′ untranslated region of alfalfa mosaic virus RNA 4. Journal of Virology 68:2194–2205
    [Google Scholar]
  11. Langereis K., Mugnier M.-A., Cornelissen B. J. C., Pinck L., Bol J. F. 1986; Variable repeats and poly(A) stretches in the leader sequence of alfalfa mosaic virus RNA 3. Virology 154:409–414
    [Google Scholar]
  12. Lovisolo O. 1993; Agro-ecology and centers of origin of graft-transmissible diseases of citrus. Proceedings of the International Organization of Citrus Virologists 12:406–411
    [Google Scholar]
  13. Mandl C. W., Heinz F. X., Puchhammer-Stockl E., Kunz C. 1991; Sequencing the termini of capped viral RNA by 5′–3′ ligation and PCR. BioTechniques 10:484–486
    [Google Scholar]
  14. Mink G. I. 1991; Ilarvirus vectors. Advances in Disease Vector Research 9:261–281
    [Google Scholar]
  15. Neeleman L., van der Kuyl A. C., Bol J. F. 1991; Role of alfalfa mosaic virus coat protein gene in symptom formation. Virology 181:687–693
    [Google Scholar]
  16. Ravelonandro M., Pinck M., Pinck L. 1984; Complete nucleotide sequence of RNA 3 for alfalfa mosaic virus strain S. Biochimie 66:395–402
    [Google Scholar]
  17. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Sánchez-Navarro J. A., Pállas V. 1994; Nucleotide sequence of apple mosaic ilarvirus RNA 4. Journal of General Virology 75:1441–1445
    [Google Scholar]
  19. Sehnke P. C., Mason A. M., Hood S. J., Lister R. M., Johnson J. E. 1989; A “zinc-finger’’-type binding domain in tobacco streak virus coat protein. Virology 168:48–56
    [Google Scholar]
  20. Shukla D. D., Ward C. W. 1988; Amino acid sequence homology of coat proteins as a basis for identification and classification of the potyvirus group. Journal of General Virology 69:2703–2710
    [Google Scholar]
  21. Symons R. H. 1985; Viral genome structure. In The Plant Viruses vol 1 Polyhedral Virions with Tripartite Genomes pp 57–81 Edited by Francki R. I. B. New York: Plenum Press;
    [Google Scholar]
  22. Zuidema D., Jaspars E. M. J. 1984; Comparative investigations on the coat protein binding sites of the genomic RNAs of alfalfa mosaic virus and tobacco streak viruses. Virology 135:43–52
    [Google Scholar]
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