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Journal of General Virology header logo Journal of General Virology

Volume 76, Issue 4

The cellular RING finger protein PML is not a functional counterpart of the herpes simplex virus type 1 RING finger protein Vmw110 No Access

Abstract

Herpes simplex virus type 1 (HSV-1) immediate early protein Vmw110 (also known as ICP0) is required for the fully efficient expression of viral genes during onset of lytic growth and for normal reactivation from latency. Both Vmw110 and the cellular protein PML are members of the RING finger family of zinc binding domain proteins, a family which includes an increasing number of examples from a wide evolutionary range. The function of the RING finger domain is unknown, and the question arises whether the RING finger (like several other examples of conserved domains) fulfils similar functions in these diverse proteins. Another link between Vmw110 and PML is that at early times of HSV-1 infection Vmw110 migrates to distinct nuclear structures which contain the PML protein. In order to test the possibility that PML and Vmw110, or their RING finger domains, fulfil similar functions, we have constructed recombinant viruses that express either intact PML, or a chimeric Vmw110 protein which contains the PML RING finger in place of its own. The results indicate that the PML and Vmw110 RING fingers are not functionally interchangeable, and that PML is not a cellular functional counterpart of Vmw110.

  • Received:
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© The Journal of General Virology 1995
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/content/journal/jgv/10.1099/0022-1317-76-4-791
1995-04-01
2025-04-26
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References

  1. Barlow P. N., Luisi B., Milner A., Elliott M., Everett R. D. 1994; Structure of the C3HC4 domain by 1H-nuclear magnetic resonance spectroscopy. Journal of Molecular Biology 237:201–211
     [Google Scholar]
  2. Cai W., Schaffer P. A. 1989; Herpes simplex virus type 1 ICPO plays a critical role in the de novo synthesis of infectious virus following transfection of viral DNA. Journal of Virology 63:4579–4589
     [Google Scholar]
  3. Cai W., Astor T. D., Liptak L. M., Cho C., Coen D., Schaffer P. A. 1993; The herpes simplex virus type 1 regulatory protein ICPO enhances replication during acute infection and reactivation from latency. Journal of Virology 67:7501–7512
     [Google Scholar]
  4. Clements G. B., Stow N. D. 1989; A herpes simplex virus type 1 mutant containing a deletion within immediate-early gene 1 is latency competent in mice. Journal of General Virology 70:2501–2506
     [Google Scholar]
  5. De The H., Lavau C., Marchio A., Chomienne C., Degos L., Dejean A. 1991; The PML-RAR alpha fusion mRNA generated by the t(15; 17) translocation in promyelocytic leukaemia encodes a functionally altered RAR. Cell 66:675–684
     [Google Scholar]
  6. Dyck J. A., Maul G. G., Miller W. H. Jr, Chen J. D., Kakizuka A., Evans R. M. 1994; A novel macromolecular structure is a target of the promyelocytic-retinoic acid receptor oncoprotein. Cell 76:333–343
     [Google Scholar]
  7. Everett R. D. 1986; A detailed mutational analysis of Vmw110, a trans-acting transcriptional activator encoded by herpes simplex virus type 1. EMBP Journal 6:2069–2076
     [Google Scholar]
  8. Everett R. D. 1988; Analysis of the functional domains of herpes simplex virus type 1 immediate-early polypeptide Vmw110. Journal of Molecular Biology 202:87–96
     [Google Scholar]
  9. Everett R. D. 1989; Construction and characterization of herpes simplex virus type 1 mutants with defined lesions in immediate early gene 1. Journal of General Virology 70:1185–1202
     [Google Scholar]
  10. Everett R. D., Maul G. G. 1994; HSV-1 IE protein Vmw110 causes redistribution of PML. EMBO Journal 13: in press
    [Google Scholar]
  11. Everett R. D., Preston C. M., Stow N. D. 1991; Functional and genetic analysis of the role of Vmw110 in herpes simplex virus replication. In Herpes Virus Transcription and its Regulation Edited by Wagner E. K. Boca Raton: CRC Press;
     [Google Scholar]
  12. Everett R. D., Barlow P. N., Milner A., Luisi B., Orr A., Hope R. G., Lyon D. 1993a; A novel arrangement of zinc binding residues and secondary structure in the C3HC4 motif of an alpha herpes virus protein family. Journal of Molecular Biology 234:1038–1047
     [Google Scholar]
  13. Everett R. D., Cross A., Orr A. 1993b; A truncated form of herpes simplex virus type 1 immediate-early protein Vmw110 is expressed in a cell-type depenent manner. Virology 197:751–756
     [Google Scholar]
  14. Freemont P. S. 1993; The RING finger. A novel protein sequence motif related to the zinc finger. Annals of the New York Academy of Sciences 684:174–192
     [Google Scholar]
  15. Freemont P. S., Hanson I. M., Trowsdale J. 1991; A novel cysteine-rich motif. Cell 64:483–484
     [Google Scholar]
  16. Goddard A. D., Borrow J., Freemont P. S., Solomon E. 1991; Characterisation of a zinc finger gene disrupted by the t(15;17) in acute promyelocytic leukaemia. Science 254:1371–1374
     [Google Scholar]
  17. Harris R. A., Everett R. D., Zhu X., Silverstein S., Preston C. M. 1989; Herpes simplex virus type 1 immediate-early protein Vmw110 reactivates latent herpes simplex virus type 2 in an in vitro latency system. Journal of Virology 63:3513–3515
     [Google Scholar]
  18. Kakizuka A., Miller W. H., Umesono K., Warrell R. P., Frankel S. R., Murty V. V. V. S., Dmitrovsky E., Evans R. M. 1991; Chromosomal translocation t(l 5; 17) in acute promyelocytic leukaemia fuses RAR alpha with a novel putative transcription factor PML. Cell 66:663–674
     [Google Scholar]
  19. Kastner P., Perez A., Lutz Y., Rochette-Egly C., Gaub M. P., Durand B., Lanotte M., Berger R., Chambon P. 1992; Structure, localisation and transcriptional properties of two classes of retinoic acid receptor alpha fusion proteins in acute promyelocytic leukaemia (APL): structural similarities with a new family of oncoproteins. EMBO Journal 11:629–642
     [Google Scholar]
  20. Koken M. H. M., Puvion-Dutilleul F., Guillemin M. C., Viron A., Linares-Cruz G., Stuurman N., de Jong L., Szostecki C., Calvo F., Chomienne C., Degos L., Puvion E., de The H. 1994; The t(15; 17) translocation alters a nuclear body in a retinoic acid reversible fashion. EMBO Journal 13:1073–1083
     [Google Scholar]
  21. Lieb D. A., Coen D. M., Bogard C. L., Hicks K. A., Yager D. R., Knipe D. M., Tyler K. L., Schaffer P. A. 1989; Immediate-early regulatory gene mutants define different stages in the establishment and reactivation of herpes simplex virus latency. Journal of Virology 63:759–768
     [Google Scholar]
  22. Maul G. G., Everett R. D. 1994; The nuclear location of PML, a cellular member of the C3HC4 zinc-binding domain protein family, is rearranged during herpes simplex virus infection by the C3HC4 viral protein ICPO. Journal of General Virology 75:1223–1233
     [Google Scholar]
  23. Maul G. G., Guldner H. H., Spivack J. G. 1993; Modification of discrete nuclear domains induced by herpes simplex virus type 1 immediate early gene 1 product ICPO. Journal of General Virology 74:2679–2690
     [Google Scholar]
  24. Mu Z.-M., Chin K.-V., Liu J.-H., Lozano G., Chang K. S. 1994; PML, a growth suppressor disrupted in acute promyelocytic leukaemia. Molecular and Cellular Biology 14:6858–6867
     [Google Scholar]
  25. Pandolfi P. P., Alcalay M., Fagioli M., Zangrilli D., Mencarelli A., Diverio D., Biondi A., Lo Coco F., Rambaldi A., Grignani F., Rochette-Egly C., Gaube M.-P., Chambon P., Pellici P. G. 1992; Genomic variability and alternative splicing generate multiple PML/RAR alpha transcripts that encode aberrant PML proteins and PML/RAR alpha isoforms in acute promyelocytic leukaemia. EMBO Journal II:1397–1407
     [Google Scholar]
  26. Perez A., Kastner P., Sethi S., Lutz Y., Reibel C., Chambon P. 1993; PMLRAR homodimers: distinct DNA binding properties and heteromeric interactions with RXR. EMBO Journal 12:3171–3182
     [Google Scholar]
  27. Stow N. D., Stow E. C. 1986; Isolation and characterization of a herpes simplex virus type 1 mutant containing a deletion within the gene encoding the immediate early polypeptide Vmw110. Journal of General Virology 67:2571–2585
     [Google Scholar]
  28. Stuurman N., DeGraaf A., Josso A., Humbel B., DeYong L., van Driel A. 1992; A monoclonal antibody recognising nuclear matrix-associated nuclear bodies. Journal of Cell Science 101:773–784
     [Google Scholar]
  29. Weis K., Rambaud S., Lavau C., Jansen J., Carvalho T., Carmo-Fonseca M., Lamond A., Dejean A. 1994; Retinoic acid regulates aberrant nuclear localization of PML-RAR alpha in acute promyelocytic leukaemia cells. Cell 76:345–356
     [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-76-4-791
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The cellular RING finger protein PML is not a functional counterpart of the herpes simplex virus type 1 RING finger protein Vmw110
J. Gen. Virol. 76, 791 (1995); https://doi.org/10.1099/0022-1317-76-4-791
/content/journal/jgv/10.1099/0022-1317-76-4-791
/content/journal/jgv/10.1099/0022-1317-76-4-791
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