1887

Abstract

Intranuclear structure was studied in influenza virus-infected cells by immunofluorescence microscopy with antibodies specific for fibrillarin, the splicing factor SC35 and the autoantigen p80 coilin. In the course of the infection, an increase in the number of coiled bodies was observed, with a parallel decrease in their size. In addition, the normal speckled pattern of the SC35 factor was altered to generate a more punctate distribution. However, no alteration was observed in the fibrillarin staining pattern. Since an alteration in the splicing of both viral and cellular mRNAs upon expression of influenza virus NS1 protein has been reported previously, the possible effects of NS1 expression on intranuclear structure were assayed. The increase in the coiled body numbers was not specific for the expression of NS1 protein, but alterations in the nuclear location of small ribonucleoprotein particles, as determined by immunofluorescence with an anti-Sm serum or the SC35 splicing factor, were produced by the sole expression of NS1 protein. These results correlate with the previously reported inhibition of splicing induced by NS1 protein expression and suggest an interaction of this influenza virus protein with the cellular splicing machinery.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-4-1001
1995-04-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/4/JV0760041001.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-4-1001&mimeType=html&fmt=ahah

References

  1. Bridge E., Carmo-Fonseca M., Lamond A. I., Petterson U. 1993; Nuclear organization of splicing small nuclear ribonucleo-proteins in adenovirus-infected cells. Journal of Virology 67:5792–5802
    [Google Scholar]
  2. Carmo-Fonseca M., Tollervey D., Pepperkok R., Barabino S. M. L., Merdes A., Brunner C., Zamore P. D., Green M. R., Hurt E., Lamond A. I. 1991; Mammalian nuclei contain foci which are highly enriched in components of the pre-mRNA splicing machinery. EMBO Journal 10:195–206
    [Google Scholar]
  3. Fortes P., Beloso A., Orti′n J. 1994; Influenza virus NS1 protein inhibits pre-mRNA splicing and blocks RNA nucleocytoplasmic transport. EMBO Journal 13:704–712
    [Google Scholar]
  4. Green M. R. 1991; Biochemical mechanisms of constitutive and regulated pre-mRNA splicing. Annual Review of Cell Biology 7:559–599
    [Google Scholar]
  5. Hatada E., Fukuda R. 1992; Binding of influenza A virus NS1 protein to dsRNA in vitro . Journal of General Virology 73:3325–3329
    [Google Scholar]
  6. Hatada E., Takizawa T., Fukuda R. 1992; Specific binding of influenza A virus NS1 protein to the virus minus-sense RNA in vitro . Journal of General Virology 13:17–25
    [Google Scholar]
  7. Herz C., Stavnezer E., Krug R. M., Gurney T. 1981; Influenza virus, an RNA virus, synthesizes its messenger RNA in the nucleus of infected cells. Cell 26:391–400
    [Google Scholar]
  8. Inglis S. C., Brown C. M. 1981; Spliced and unspliced RNAs encoded by virion RNA segment 7 of influenza virus. Nucleic Acids Research 9:2727–2740
    [Google Scholar]
  9. Inglis S. C., Barret T., Brown C. M., Almond J. W. 1979; The smallest genome RNA segment of influenza virus contains two genes that may overlap. Proceedings of the National Academy of Sciences, USA 76:3790–3794
    [Google Scholar]
  10. Jiménez-García L. F., Spector D. L. 1993; In vivo evidence that transcription and splicing are coordinated by a recruiting mechanism. Cell 73:47–59
    [Google Scholar]
  11. Krug R. M., Broni B. A., Bouloy M. 1979; Are the 5′-ends of influenza viral mRNAs synthesized in vivo donated by host mRNAs?. Cell 18:329–334
    [Google Scholar]
  12. Lamb R. A., Choppin P. W. 1979; Segment 8 of the influenza virus genome is unique in coding for two polypeptides. Proceedings of the National Academy of Sciences, USA 76:4908–4912
    [Google Scholar]
  13. Lamb R. A., Lai C. J., Choppin P. W. 1981; Sequences of mRNAs derived from genome RNA segment 7 of influenza virus: collinear and interrupted mRNAs code for overlapping proteins. Proceedings of the National Academy of Sciences, USA 78:4170–4174
    [Google Scholar]
  14. Lamond A. I., Carmo-Fonseca M. 1993; The coiled body. Trends in Cell Biology 3:198–204
    [Google Scholar]
  15. López J. A., Guillen M., Sanchez F. A., Melero J. A. 1986; An antigen-binding assay to determine the specificity of monoclonal antibodies against influenza virus and mapping of epitopes. Journal of Virological Methods 13:255–264
    [Google Scholar]
  16. López-Turiso J. A., Martínez C., Tanaka T., Ortín J. 1990; The synthesis of influenza virus negative-strand RNA takes place in insoluble complexes present in the nuclear matrix fraction. Virus Research 16:325–337
    [Google Scholar]
  17. Martin T. E., Barghusen S. C., Leser G. P., Spear P. G. 1987; Redistribution of nuclear ribonucleoprotein antigens during herpes simplex virus infection. Journal of Cell Biology 105:2069–2082
    [Google Scholar]
  18. Nemeroff K. E., Utans U., Kramer A., Krug R. M. 1992; Identification of cw-acting intron and exon sequences in influenza virus NS1 mRNA that inhibit splicing and cause the formation of aberrantly sedimenting presplicing complexes. Molecular and Cellular Biology 12:962–970
    [Google Scholar]
  19. Newman A. J., Norman C. 1992; U5 sn RNA interacts with exon sequences at 5′ and 3′ slice sites. Cell 68:743–754
    [Google Scholar]
  20. O’Keefe R. T., Mayeda A., Sadowski C. L., Krainer A. R., Spector D. L. 1994; Disruption of pre-mRNA splicing in vivo results in reorganization of splicing factors. Journal of Cell Biology 124:249–260
    [Google Scholar]
  21. Phelan A., Carmo-Fonseca M., McLauchlan J., Lamond A. I., Clements J. B. 1993; A herpes simplex virus type 1 immediate-early product, IE63, regulates small nuclear ribonucleoprotein distribution. Proceedings of the National Academy of Sciences, USA 90:9056–9060
    [Google Scholar]
  22. Portela A., Melero J. A., de la Luna S., Orti′n J. 1986; Construction of cell lines that regulate by temperature the amplification and expression of influenza virus non-structural protein genes. EMBO Journal 5:2387–2392
    [Google Scholar]
  23. Qiu Y., Krug R. M. 1994; The influenza virus NS1 protein is a poly(A)-binding protein that inhibits nuclear export of mRNAs containing poly(A). Journal of Virology 68:2425–2432
    [Google Scholar]
  24. Schwer B., Guthrie C. 1991; PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome. Nature 349:494–499
    [Google Scholar]
  25. Shapiro G. I., Krug R. M. 1988; Influenza virus RNA replication in vitro: synthesis of viral template RNAs and virion RNAs in the absence of an added primer. Journal of Virology 62:2285–2290
    [Google Scholar]
  26. Smith D. B., Inglis S. C. 1985; Regulated production of an influenza virus spliced mRNA mediated by virus-specific products. EMBO Journal 4:2313–2319
    [Google Scholar]
  27. Spector D. L. 1993; Nuclear organization of pre-mRNA splicing. Current Opinion in Cell Biology 5:442–448
    [Google Scholar]
  28. Spector D. L., Fu X.-D., Maniatis T. 1991; Associations between distinct pre-mRNA splicing components and the cell nucleus. EM BO Journal 10:3467–3481
    [Google Scholar]
  29. Valcárcel J., Portela A., Ortín J. 1991; Regulated M1 mRNA splicing in influenza virus-infected cells. Journal of General Virology 72:1301–1308
    [Google Scholar]
  30. Zhang M., Zamore P. D., Carmo-Fonseca M., Lamond A. I., Green M. R. 1992; Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit. Proceedings of the National Academy of Sciences, USA 89:8769–8773
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-4-1001
Loading
/content/journal/jgv/10.1099/0022-1317-76-4-1001
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error