1887

Abstract

Recombinant vectors based on the type 1 human immunodeficiency virus (HIV-1) can be used to deliver genes into cells expressing the HIV receptor, CD4. We have used a transient RNA packaging system to compare the safety and efficacy of HIV-1 vector transduction by wild-type and replication-deficient helper viruses. Helper virus-free vector transfer was consistently achieved when the helper virus and genes were expressed from separate plasmids such that two recombination events were required to form an infectious genome. Other forms of attenuation, such as deletion of the 5′ ψ region, were inadequate to prevent helper virus transmission. Vector transduction by the wild-type and non-replicating helper viruses occurred with comparable efficiency except in instances where efficient vector RNA expression was dependent upon transactivating factors supplied by the helper virus. These data demonstrate the feasibility of safe gene transfer using HIV-1 vectors.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-3-691
1995-03-01
2022-05-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/3/JV0760030691.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-3-691&mimeType=html&fmt=ahah

References

  1. Buchschacher G. L., Panganiban A. T. 1992; Human immunodeficiency virus vectors for inducible expression of foreign genes. Journal of Virology 66:2731–2739
    [Google Scholar]
  2. Cosset F. L., Legras C., Chebloune Y., Savatier P., Thoraval P., Thomas J. L., Samarut J., Nigon V. M., Verdier G. 1990; A new avian leukosis virus-based packaging cell line that uses two separate transcomplementing helper genomes. Journal of Virology 64:1070–1078
    [Google Scholar]
  3. Dougherty J. P., Wisniewski R., Yang S., Rhode B. W., Temin W. 1989; New retrovirus helper cells with almost no nucleotide sequence homology to retrovirus vectors. Journal of Virology 63:3209–3212
    [Google Scholar]
  4. Hayashi T., Shioda T., Iwakura Y., Shibuta H. 1992; RNA packaging signal of human immunodeficiency virus type 1. Virology 188:590–599
    [Google Scholar]
  5. Johnson V. A., Byington R. E. 1990; Quantitative assays for virus infectivity. In Techniques in HIV Research pp 71–76 Edited by Aldovini A., Walker B. D. New York: Stockton Press;
    [Google Scholar]
  6. Lever A. M. L., Gottlinger H., Haseltine W., Sodroski J. 1989; Identification of a sequence required for efficient packaging of human immunodeficiency virus type 1 RNA into virions. Journal of Virology 63:4085–4087
    [Google Scholar]
  7. Lewis P., Hensel M., Emerman M. 1992; Human immunodeficiency virus infection of cells arrested in the cell cycle. EMBO Journal 11:2053–3058
    [Google Scholar]
  8. Linial M., Miller A. D. 1990; RNA Packaging. In Retroviruses: Strategies of Replication pp 125–152 Edited by Swanstrom R., Vogt P. K. Berlin: Springer-Verlag;
    [Google Scholar]
  9. Miller A. D. 1990; Retrovirus packaging cells. Human Gene Therapy 1:5–14
    [Google Scholar]
  10. Potts B. J. 1990; ‘Mini’ reverse transcriptase (RT) assay. In Techniques in HIV Research pp 102–106 Edited by Aldovini A., Walker B. D. New York: Stockton Press;
    [Google Scholar]
  11. Richardson J. H., Child L. A., Lever A. M. L. 1993; Packaging of human immunodeficiency virus type 1 RNA requires cA-acting sequences outside the 5′ leader region. Journal of Virology 67:3997–4005
    [Google Scholar]
  12. Rosen C. A., Sodroski J. G., Campbell K., Haseltine W. A. 1986; Construction of recombinant murine retroviruses that express the human T-cell leukemia virus type III trans-activator genes. Journal of Virology 57:379–384
    [Google Scholar]
  13. Selden R. F. 1987; Transfection using DEAE-dextran. In Current Protocols in Molecular Biology vol 1 unit 9.2. Edited by Ausubel R., Brent R. E., Kingston D. D., Moore J. G., Seidman J. A., Smith J. A., Struhl K. New York: Wiley Interscience;
    [Google Scholar]
  14. Shimada T., Fujii H., Mitsuya H., Nienhuis A. W. 1991; Targeted and highly efficient gene transfer into CD4+ cells by a recombinant human immunodeficiency virus retroviral vector. Journal of Clinical Investigation 88:1043–1047
    [Google Scholar]
  15. Weinberg J. B., Matthwews T. J., Cullen B. R., Malim M. H. 1991; Productive human immunodeficiency virus type 1 (HIV-1) infection of non proliferating human monocytes. Journal of Experimental Medicine 174:1477–1482
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-3-691
Loading
/content/journal/jgv/10.1099/0022-1317-76-3-691
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error