1887

Abstract

Faba bean necrotic yellows virus (FBNYV) has a circular ssDNA genome possibly consisting of several components of about 1 kb each. The complete nucleotide sequence of one component of FBNYV (FBNYV DNA 1) containing a putative replicase gene is presented. This component consists of 1002 nucleotides and, in the virion orientation, contains one large open reading frame (ORF1) potentially encoding a 32.3 kDa replicase with the NTP-binding motif GGEGKS. No obvious functions could be assigned to two smaller ORFs (7.4 and 9.3 kDa) occurring in the complementary orientation. Amino acid sequence comparisons of the putative replicase of FBNYV with that of other similar ssDNA viruses yielded higher homologies to subterranean clover stunt virus than to banana bunchy top and coconut foliar decay viruses. A potential stem-loop structure and a TATA box were identified within the noncoding region. Two oligonucleotides derived from FBNYV DNA 1 were used for direct sequencing of the virion ssDNA to determine its virion polarity and for amplifying part of this component by immunocapture PCR in extracts from FBNYV-infected plants.

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1995-02-01
2024-04-24
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References

  1. Boevink P., Chu P., Keese P. 1993; Subterranean clover stunt virus - a hypervariable DNA virus. IXth International Congress of Virology Abstracts p 353 abstract P68-1
    [Google Scholar]
  2. Burns T. M., Harding R. M., Hafner G., Beetham P., Dale J. L. 1993; Single-stranded DNA genome organisation of banana bunchy top virus. 6th International Congress on Plant Pathology Montreal, abstract no. 17.2.21
    [Google Scholar]
  3. Burns T. M., Harding R. M., Dale J. L. 1994; Evidence that banana bunchy top virus has a multiple component genome. Archives of Virology 137:371–380
    [Google Scholar]
  4. Chu P. W. G., Helms K. 1988; Novel virus-like particles containing circular single-stranded DNA associated with subterranean clover stunt disease. Virology 167:38–49
    [Google Scholar]
  5. Chu P. W. G., Keese P., Qiu B. S., Waterhouse P. M., Gerlach W. L. 1993; Putative full-length clones of the genomic DNA segments of subterranean clover stunt virus and identification of the segment coding for the viral coat protein. Virus Research 27:161–171
    [Google Scholar]
  6. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  7. Faria J. C., Gilbertson R. L., Hanson S. F., Morales F. J., Ahlquist P., Loniello A. O., Maxwell D. P. 1994; Bean golden mosaic geminivirus type II isolates from the Dominican Republic and Guatemala: nucleotide sequences, infectious pseudorecombinants, and phylogenetic relationships. Phytopathology 84:321–329
    [Google Scholar]
  8. Fontes E. P. B., Gladfelter H. J., Schaffer R. L., Petty I. T. D., Hanley-Bowdoin L. 1994; Geminivirus replication origins have a modular organization. Plant Cell 6:405–416
    [Google Scholar]
  9. Gorbalenya A. E., Koonin E. V., Wolf Y. I. 1990; A new superfamily of putative NTP-binding domains encoded by genomes of small DNA and RNA viruses. FEBS Letters 262:145–148
    [Google Scholar]
  10. Harding R. M., Burns T. M., Dale J. L. 1991; Virus-like particles associated with banana bunchy top disease contain small single-stranded DNA. Journal of General Virology 72:225–230
    [Google Scholar]
  11. Harding R. M., Burns T. M., Hafner G., Dietzgen R. G., Dale J. L. 1993; Nucleotide sequence of one component of the banana bunchy top virus genome contains a putative replicase gene. Journal of General Virology 74:323–328
    [Google Scholar]
  12. Katul L., Vetten H. J., Maiss E., Makkouk K. M., Lesemann D.-E., Casper R. 1993; Characterisation and serology of virus-like particles associated with faba bean necrotic yellows. Annals of Applied Biology 123:629–647
    [Google Scholar]
  13. Lazarowitz S. G. 1987; The molecular characterization of geminiviruses. Plant Molecular Biology Reporter 4:177–192
    [Google Scholar]
  14. Lazarowitz S. G., Wu L. C., Rogers S. G., Elmer J. S. 1992; Sequence-specific interaction with the viral AL1 protein identifies a geminivirus DNA replication origin. Plant Cell 4:799–809
    [Google Scholar]
  15. Lipman D. J., Pearson W. R. 1985; Rapid and sensitive protein similarity searches. Science 227:1435–1441
    [Google Scholar]
  16. Randles J. W., Hanold D. 1989; Coconut foliar decay virus particles are 20-nm icosahedra. Intervirology 30:177–180
    [Google Scholar]
  17. Rohde W., Randles J. W., Langridge P., Hanold D. 1990; Nucleotide sequence of a circular single-stranded DNA associated with coconut foliar decay virus. Virology 176:648–651
    [Google Scholar]
  18. Rothnie H. M., Reid J., Hohn T. 1994; The contribution of AAUAAA and the upstream element UUUGUA to the efficiency of mRNA 3′-end formation in plants. EMBO Journal 13:2200–2210
    [Google Scholar]
  19. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  20. Sano Y., Isogai M., Satoh S., Kojima M. 1993; Small virus-like particles containing single-stranded DNAs associated with milk vetch dwarf disease in Japan. 6th International Congress on Plant Pathology Montreal, abstract no. 17.1.27
    [Google Scholar]
  21. Surin B., Larkin P., Boevink P., Chu P., Keese P. 1993; The subterranean clover stunt virus genome consists of microchromosomes encoding single ORFs. IXth International Congress of Virology Abstracts p 333 abstract P62-1
    [Google Scholar]
  22. Thomas J. E., Dietzgen R. G. 1991; Purification, characterization and serological detection of virus-like particles associated with banana bunchy top disease in Australia. Journal of General Virology 72:217–224
    [Google Scholar]
  23. Wetzel T., Candresse T., Macquaire G., Ravelonandro M., Dunez J. 1992; A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection. Journal of Virological Methods 39:27–37
    [Google Scholar]
  24. Yeh H. H., Su H. J., Chao Y. C. 1994; Genome characterization and identification of viral-associated dsDNA component of banana bunchy top virus. Virology 198:645–652
    [Google Scholar]
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