%0 Journal Article %A Labonté, Patrick %A Mounir, Samir %A Talbot, Pierre J. %T Sequence and expression of the ns2 protein gene of human coronavirus OC43 %D 1995 %J Journal of General Virology, %V 76 %N 2 %P 431-435 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-76-2-431 %I Microbiology Society, %X The complete nucleotide sequence of the ns2 gene of human coronavirus OC43 (HCV-OC43) was determined. Sequence analysis revealed an open reading frame that could encode a protein of 278 amino acids, with an estimated molecular mass of 32.2 kDa. Six potential phosphorylation sites are present but no sites of N-glycosylation were found. The amino acid sequence of the HCV-OC43 ns2 protein shows 92% identity with that of the Mebus strain of bovine coronavirus (BCV). However, a stretch of nine consecutive amino acids near the C terminus is completely different, causing it to be very hydrophilic, which contrasts with the hydrophobic nature of this region in BCV. As shown by immunofluorescence with a monospecific antiserum, the ns2 protein was expressed in the cytoplasm of HCV-OC43-infected HRT-18 cells. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-76-2-431