1887

Abstract

The fusion (F) and haemagglutinin—neuraminidase (HN) glycoproteins of mumps virus (MuV) have been produced in CV1 cells via vaccinia virus recombinants. Recombinant proteins accumulated in infected cells and were glycosylated. Upon reduction, the F protein product was completely converted into its subunits. Hamsters infected with vaccinia recombinants expressing either the F or HN proteins produced antibodies recognizing MuV antigens and neutralizing MuV infectivity . These antibodies provided protection against MuV-induced encephalitis in newborn hamsters.

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/content/journal/jgv/10.1099/0022-1317-76-2-421
1995-02-01
2022-05-29
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References

  1. Brown E. G., Furesz J., Dimock K., Yarosh W., Coutreras G. 1991; Nucleotide sequence analysis of Urabe Mumps vaccine strain that caused meningitis in vaccine recipients. Vaccine 9:840–842
    [Google Scholar]
  2. Chakrabarti S., Brechling K., Moss B. 1985; Vaccinia virus expression vector: coexpression of β-galactosidase provides visual screening of recombinant virus plaques. Molecular and Cellular Biology 5:3403–3409
    [Google Scholar]
  3. Elango N., Varsanyi T. M., Kövamees J., Norrby E. 1989; The mumps virus fusion protein mRNA sequence and homology among the paramyxoviridae proteins. Journal of General Virology 70:801–807
    [Google Scholar]
  4. Kilham L., Overman J. R. 1953; Natural pathogenicity of mumps virus for suckling hamsters on intracerebral inoculation. Journal of Immunology 70:147–151
    [Google Scholar]
  5. Kovamees J., Norrby E., Elango N. 1989; Complete nucleotide sequence of the HN mRNA of mumps virus and comparison of paramyxovirus HN proteins. Virus Research 12:87–96
    [Google Scholar]
  6. Löve A., Rydbeck R., Utter G., Örvell C., Kristensson K., Norrby E. 1986; Monoclonal antibodies against the fusion protein are protective in necrotizing mumps meningoencephalitis. Journal of Virology 58:220–222
    [Google Scholar]
  7. Mackett M., Smith G. L. 1986; Vaccinia virus expression vectors. Journal of General Virology 67:2067–2082
    [Google Scholar]
  8. Merz D. C., Wolinsky J. S. 1981; Biochemical features of mumps virus neuraminidase and their relationship with pathogenicity. Virology 114:218–227
    [Google Scholar]
  9. Nokes D. J., Anderson R. M. 1991; Vaccine safety versus vaccine efficacy in mass immunisation programmes. Lancet 338:1309–1312
    [Google Scholar]
  10. Örvell C. 1984; The reaction of monoclonal antibodies with structural proteins of mumps virus. Journal of Immunology 132:2622–2629
    [Google Scholar]
  11. Örvell C. 1988; Paramyxoviridae: mumps virus. In Laboratory diagnosis of infectious diseases Edited by Balows A., Hausler W. J., Lennette E. H. pp 507–524 Berlin, Heidelberg & New York: Springer-Verlag;
    [Google Scholar]
  12. Waxham Μ. N., Server A. C., Goodman Η. M., Wolinsky J. S. 1987; Cloning and sequencing of the mumps virus fusion protein gene. Virology 159:381–388
    [Google Scholar]
  13. Waxham Μ. N., Aronowski J., Server A. C., Wolinsky J. S., Smith J. A., Goodman Η. M. 1988; Sequence determination of the mumps virus HN gene. Virology 164:318–325
    [Google Scholar]
  14. Wolinsky J. S., Waxham M. N. 1990; Mumps virus. In Virology Edited by Fields B. Ν., Knippe D. M. 2nd edn pp 989–1011 New York: Raven Press;
    [Google Scholar]
  15. Wolinsky J. S., Baringer J. R., Margolis G., Kilham L. 1974; Ultrastructure of mumps virus replication in newborn hamster central nervous system. Laboratory Investigation 31:403–412
    [Google Scholar]
  16. Wolinsky J. S., Waxham Μ. N., Server A. C. 1985; Protective effects of glycoprotein-specific monoclonal antibodies on the course of experimental mumps virus meningoencephalitis. Journal of Virology 53:727–734
    [Google Scholar]
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