@article{mbs:/content/journal/jgv/10.1099/0022-1317-76-12-3165, author = "Matano, Tetsuro and Odawara, Takashi and Iwamoto, Aikichi and Yoshikura, Hiroshi", title = "Targeted infection of a retrovirus bearing a CD4-Env chimera into human cells expressing human immunodeficiency virus type 1", journal= "Journal of General Virology", year = "1995", volume = "76", number = "12", pages = "3165-3169", doi = "https://doi.org/10.1099/0022-1317-76-12-3165", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-76-12-3165", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "We constructed a hybrid Moloney murine leukaemia virus (MoMLV) bearing both a chimeric CD4 and the wild-type MoMLV envelope protein (Env) on its surface. The chimeric molecule, consisting of a surface domain of CD4 and the C-terminal two-thirds of MLV Env, was expressed on the cell surface. When expressed in MoMLV-infected cells, the CD4-Env chimera was incorporated into the virion as efficiently as the wild-type MoMLV Env. The hybrid MoMLV could infect human HeLa cells (although not with high efficiency) only if the cells were expressing human immunodeficiency virus type 1 genome. This method of ligand incorporation into a virion may lead to a development of a cell-specific retroviral vector for targeting gene therapy.", }