1887

Abstract

We constructed a hybrid Moloney murine leukaemia virus (MoMLV) bearing both a chimeric CD4 and the wild-type MoMLV envelope protein (Env) on its surface. The chimeric molecule, consisting of a surface domain of CD4 and the C-terminal two-thirds of MLV Env, was expressed on the cell surface. When expressed in MoMLV-infected cells, the CD4-Env chimera was incorporated into the virion as efficiently as the wild-type MoMLV Env. The hybrid MoMLV could infect human HeLa cells (although not with high efficiency) only if the cells were expressing human immunodeficiency virus type 1 genome. This method of ligand incorporation into a virion may lead to a development of a cell-specific retroviral vector for targeting gene therapy.

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1995-12-01
2021-10-25
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References

  1. Adachi A., Gendelman H. E., Koenig S., Folks T., Willey R., Rabson A., Martin M. A. 1986; Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone. Journal of Virology 59:284–291
    [Google Scholar]
  2. Arthur L., Bess J., Sowder R., Benveniste R., Mann D., Chermann J.-C., Henderson L. 1992; Cellular proteins bound to immunodeficiency viruses: implications for pathogenesis and vaccines. Science 258:1935–1938
    [Google Scholar]
  3. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  4. Dorfman T., Mammano F., Haseltine W. A., Gottlinger H. G. 1994; Role of the matrix protein in the virion association of the human immunodeficiency virus type 1 envelope glycoprotein. Journal of Virology 68:1689–1696
    [Google Scholar]
  5. Einfeld D., Hunter E. 1988; Oligomeric structure of a prototype retrovirus glycoprotein. Proceedings of the National Academy of Sciences, USA 85:8688–8692
    [Google Scholar]
  6. Etienne-Julan M., Roux P., Carillo S., Jeanteur P., Piechaczyk M. 1992; The efficiency of cell targeting by recombinant retroviruses depends on the nature of the receptor and the composition of the artificial cell-virus linker. Journal of General Virology 73:3251–3255
    [Google Scholar]
  7. Friedman T. 1989; Progress toward gene therapy. Science 244:1275–1281
    [Google Scholar]
  8. Jones D. S., Nemoto F., Kuchino Y., Masuda M., Yoshikura H., Nishimura S. 1989; The effect of specific mutations at and around the gag-pol gene junction of Moloney murine leukemia virus. Nucleic Acids Research 17:5933–5945
    [Google Scholar]
  9. Kasahara N., Dozy A. M., Kan Y. W. 1994; Tissue-specific targeting of retroviral vectors through ligand-receptor interactions. Science 266:1373–1376
    [Google Scholar]
  10. Koch W., Hunsmann G., Friedrich R. 1983; Nucleotide sequence of the envelope gene of Friend murine leukemia virus. Journal of Virology 45:1–9
    [Google Scholar]
  11. Maddon P. J., Littman D. R., Godfrey M., Maddon D. E., Chess L., Axel R. 1985; The isolation and nucleotide sequence of a cDNA encoding the T cell surface protein T4: a new member of the immunoglobulin gene family. Cell 42:93–104
    [Google Scholar]
  12. Matano T., Odawara T., Ohshima M., Yoshikura H., Iwamoto A. 1993; Irani-Dominant interference with virus infection at two different stages by a mutant envelope protein of Friend murine leukemia virus. Journal of Virology 67:2026–2033
    [Google Scholar]
  13. Matano T., Odawara T., Ohshima M., Iwamoto A., Yoshikura H. 1994; Interaction between the dominant negative mutant and the wild-type envelope proteins of Friend murine leukemia virus. Journal of Virology 68:6079–6082
    [Google Scholar]
  14. Niwa H., Yamamura K., Miyazaki J. 1991; Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193–199
    [Google Scholar]
  15. Patuleia M. C., Friend C. 1967; Tissue culture studies on murine virus-induced leukemia cells: isolation of single cells in agar-liquid medium. Cancer Research 27:726–730
    [Google Scholar]
  16. Roux P., Jeanteur P., Piechaczyk M. 1989; A versatile and potentially general approach to the targeting of specific cell types by recombinant retroviruses. Proceedings of the National Academy of Sciences, USA 86:9079–9083
    [Google Scholar]
  17. Rowe W. P., Pugh W. E., Hartley J. W. 1970; Plaque assay techniques for murine leukemia viruses. Virology 42:1136–1139
    [Google Scholar]
  18. Saiki R. K., Buga Wan T. L., Horn G. T., Mullis K. B., Erlich H. A. 1986; Analysis of enzymatically amplified β-globin and HLA-DQα dna with allele-specific oligonucleotide probes. Nature 293:543–548
    [Google Scholar]
  19. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Shinnick T. M., Lerner R. A., Sutcliffe J. G. 1981; Nucleotide sequence of Moloney murine leukemia virus. Nature 324:163–166
    [Google Scholar]
  21. Suomalainen M., Garoff H. 1994; Incorporation of homologous and heterologous proteins into the envelope of Moloney murine leukemia virus. Journal of Virology 68:4879–4889
    [Google Scholar]
  22. Valsesia-Wittmann S., Drynda A., Deleage G., Aumailley M., Heard J.-M., Danos O., Verdier G., Cosset F.-L. 1994; Modifications in the binding domain of avian retrovirus envelope protein to redirect the host range of retroviral vectors. Journal of Virology 68:4609–4619
    [Google Scholar]
  23. Weiss R. A. 1993; Cellular receptors and viral glycoproteins involved in retrovirus entry. In The Retroviridae vol 2 pp 1–108 Edited by Levy J. A. New York: Plenum Press;
    [Google Scholar]
  24. Young J., Bates P., Willert K., Varmus H. 1990; Efficient incorporation of human CD4 protein into avian leukosis virus particles. Science 250:1421–1423
    [Google Scholar]
  25. Yu X., Yuan X., Matsuda Z., Lee T.-H., Essex M. 1992; The matrix protein of human immunodeficiency virus type 1 is required for incorporation of viral envelope protein into mature virions. Journal of Virology 66:4966–4971
    [Google Scholar]
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