%0 Journal Article %A Adam, Micheline %A Oualikene, Wahiba %A Cocq, Hervé Le %A Guittet, Michèle %A Eloit, Marc %T Replication-defective adenovirus type 5 as an in vitro and in vivo gene transfer vector in chickens %D 1995 %J Journal of General Virology, %V 76 %N 12 %P 3153-3157 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-76-12-3153 %I Microbiology Society, %X The capacity of E1A gene-deleted and thus replication-defective adenovirus type 5 (Ad5) to transduce foreign genes in chicken embryo fibroblasts (CEF) as well as in chickens was investigated. The lacZ and luciferase genes were successfully transduced in CEF by replication-defective Ad5, demonstrating that these cells possess receptor(s) for binding and penetration of Ad5. A single intramuscular inoculation of Ad-gD, a replication-defective Ad5 harbouring the gD gene of pseudorabies virus, in adult and 1-day-old chickens led to the production of very high titres of specific antibodies. These gD-specific antibodies persisted for at least 56 days. These results demonstrate that replication-defective Ad5, despite its mammalian origin and the deletion of the E1A gene, is a good candidate for developing non-spreading vaccines in poultry. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-76-12-3153