%0 Journal Article %A Baxi, M. K. %A Efstathiou, S. %A Lawrence, G. %A Whalley, J. M. %A Slater, J. D. %A Field, H. J. %T The detection of latency-associated transcripts of equine herpesvirus 1 in ganglionic neurons %D 1995 %J Journal of General Virology, %V 76 %N 12 %P 3113-3118 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-76-12-3113 %I Microbiology Society, %X Neural tissues from specific pathogen-free ponies that had been experimentally infected with equine herpesvirus 1 (EHV-1) were analysed by in situ hybridization. Digoxigenin-labelled EHV-1 BamHI fragments spanning almost the entire EHV-1 genome were hybridized to RNA in tissue sections from latently infected trigeminal ganglia. The BamHI E fragment detected EHV-1 RNA antisense to gene 63 (HSV-1 homologue ICP0) in a small number of neurons. Sixteen other BamHI fragments gave negative results in 20 sections tested with each fragment. Latency associated transcripts (LATs) were localized to the neuronal nuclei. EHV-1 nucleotide sequence data in the region reveals the presence of a putative EHV-1 LAT promoter that shares similar motifs with the HSV-1 LAT promoter, including the LAT promoter-binding factor, and may have a role in EHV-1 LAT expression. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-76-12-3113