1887

Abstract

Neutralization sites on swine vesicular disease virus (SVDV) have been identified by sequence analysis of neutralization-resistant mutants. Eight neutralizing monoclonal antibodies (MAbs) were produced and neutralization-resistant mutants were selected with the MAbs. Resistance of the mutants to neutralization was shown using the stab-neutralization method, and the results indicated the presence of five neutralization sites on the virus. The location of each site was identified from amino acid changes resulting from nucleotide substitutions in the mutants, and designated site 1 (residues 87 and 88 of VP1), 2a (residue 163 of VP2), 2b (residue 154 of VP2), 3a (residues 272 and 275 of VP1, 60 of VP3) and 3b (residues 70 and 233 of VP2, 73 and 76 of VP3). The locations of the amino acid substitutions at each site formed a cluster on a computer-simulated three-dimensional model of SVDV and were exposed to the surface of the virion.

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1995-12-01
2021-10-21
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References

  1. Acharya R., Fry E., Stuart D., Fox G., Rowlands D. J., Brown F. 1989; The three dimensional structure of foot and mouth disease virus at 2.9 Å resolution. Nature 337:709–716
    [Google Scholar]
  2. Blundell T., Carney D., Gardner S., Hayes F., Howlin B., Hubbard T., Overington J., Singh D. A., Sibanda B. L., Sutcliffe M. 1988; 18th Sir Hans Krebs Lecture; Knowledge-based protein modeling and design. European Journal of Biochemistry 172:513–520
    [Google Scholar]
  3. Brown F., Talbot P., Burrows R. 1973; Antigenic differences between isolates of swine vesicular disease virus and their relationship to coxsackie B5 virus. Nature 245:315–316
    [Google Scholar]
  4. Brown F., Wild T. F., Rowe L. W., Underwood B. O., Harris T. J. R. 1976; Comparison of swine vesicular disease virus and coxsackie B5 virus by serological and RNA hybridization methods. Journal of General Virology 31:231–237
    [Google Scholar]
  5. Crowther J. R., Farias S., Carpenter W. C., Samuel A. R. 1993; Identification of a fifth neutralizable site on type O foot-and-mouth disease virus following characterization of single and quintuple monoclonal antibody escape mutants. Journal of General Virology 74:1547–1553
    [Google Scholar]
  6. Ferguson M., Minor P. D., Magrath D. I., Qui Y.I-Hua, Spitz M., Schild G. C. 1984; Neutralization epitopes on poliovirus type 3 particles: an analysis using monoclonal antibodies. Journal of General Virology 65:197–201
    [Google Scholar]
  7. Filman D. J., Syed R., Chow M., MacAdam A. J., Minor P. D., Hogle J. M. 1989; Structure factors that control conformational transitions and serotype specificity in type 3 poliovirus. EMBO Journal 8:1567–1579
    [Google Scholar]
  8. Graves J. H. 1973; Serological relationship of swine vesicular disease virus and coxsackie B5 virus. Nature 245:314–315
    [Google Scholar]
  9. Graves J. H. 1986; Swine vesicular disease. In Diseases of Swine 6th edn, pp 348–352 Edited by Leman A. D. Straw B., Glock R. D., Mengeling W. L., Penny R. H. C., Scholl E. Ames: Iowa State University Press;
    [Google Scholar]
  10. Grubman M. J., Baxt B., Bachrach H. L. 1979; Foot-and-mouth-disease virion RNA: studies on the relation between the length of its 3′-poly(A) segment and infectivity. Virology 97:22–31
    [Google Scholar]
  11. Harrison S. C. 1990; Principles of virus structure. In Virology 2nd edn pp 37–62 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  12. Hogle J. M., Chow M., Filman D. J. 1985; Three-dimensional structure of poliovirus at 2.9 Å resolution. Science 229:1358–1365
    [Google Scholar]
  13. Inoue T., Suzuki T., Sekiguchi K. 1989; The complete nucleotide sequence of swine vesicular disease virus. Journal of General Virology 70:919–934
    [Google Scholar]
  14. Inoue T., Yamaguchi S., Kanno T., Sugita S., Saeki T. 1993; The complete nucleotide sequence of a pathogenic swine vesicular diseases virus isolated in Japan (J1′73) and phylogenetic analysis. Nucleic Acids Research 21:3896
    [Google Scholar]
  15. Ketterlinus R., Wiegers K., Dernick R. 1993; Revertants of poliovirus escape mutants: new insights into antigenic structures. Virology 192:525–533
    [Google Scholar]
  16. Kodama M., Ogawa T., Saito T., Tokuda G., Sasahara J., Kumagai T. 1980a; Swine vesicular disease virus isolated from healthy pigs in non-epizootic period. I. Isolation and identification. National Institute of Animal Health Quarterly (Yatahe) 20:1–10
    [Google Scholar]
  17. Kodama M., Saito T., Ogawa T., Tokuda G., Sasahara J., Kumagai T. 1980b; Swine vesicular disease virus isolated from healthy pigs in non-epizootic periods. II. Vesicular formation and virus multiplication in experimentally inoculated pigs. National Institute of Animal Health Quarterly (Yatabe) 20:123–130
    [Google Scholar]
  18. Luo M., Vriend G., Kamer G., Minor I., Arnold E., Rossman M. G., Boege U., Scraba D. G., Duke G. M., Palmenberg A. C. 1987; The atomic structure of Mengovirus at 3-0 A resolution. Science 235:182–191
    [Google Scholar]
  19. Minor P. D., Ferguson M., Evans D. M. A., Almond J. W. Icenogle J. P. 1986; Antigenic structure of polioviruses of serotypes 1, 2 and 3. Journal of General Virology 67:1283–1291
    [Google Scholar]
  20. Nardelli L., Lodetti E., Gualandi G. L., Burrows R., Goodridge D., Brown F., Cartwright B. 1968; A foot and mouth disease syndrome in pigs caused by an enterovirus. Nature 219:1275–1276
    [Google Scholar]
  21. Nomoto A., Kajigaya S., Suzuki K., Imura N. 1979; Possible point mutation sites in LSc, 2ab poliovirus RNA and a protein covalently linked to the 5′-terminus. Journal of General Virology 45:107–117
    [Google Scholar]
  22. Page G. S., Mosser A. G., Hogle J. M., Filman D. J., Rueckert R. R., Chow M. 1988; Three-dimensional structure of poliovirus serotype 1 neutralizing determinants. Journal of Virology 62:1781–1794
    [Google Scholar]
  23. Rossmann M. G., Arnold E., Erickson J. W., Frankenberger E. A., Griffith J. P., Hecht H. J., Johnson J. E., Kamer G., Lou M., Mosser A. G., Rueckert R. R., Sherry B., Vriend G. 1985; Structure of a human common cold virus and functional relationship to other picornaviruses. Nature 317:145–153
    [Google Scholar]
  24. Seechurn P., Knowles N. J., McCauley J. W. 1990; The complete nucleotide sequence of a pathogenic swine vesicular disease virus. Virus Research 16:255–274
    [Google Scholar]
  25. Sherry B., Rueckert R. 1985; Evidence for at least two dominant neutralization antigens on human rhinovirus 14. Journal of Virology 53:137–143
    [Google Scholar]
  26. Uhlig J., Wiegers K., Dernick R. 1990; A new antigenic site of poliovirus recognized by an intertypic cross-neutralizing monoclonal antibody. Virology 178:606–610
    [Google Scholar]
  27. Wiegers K., Dernick R. 1992; Molecular basis of antigenic structures of poliovirus: implications for their evolution during morphogenesis. Journal of Virology 66:4597–4600
    [Google Scholar]
  28. Wiegers K. J., Wetz K., Dernick R. 1990; Molecular basis for linkage of a continuous and discontinuous neutralization epitope on the structural polypeptide VP2 of poliovirus type 1. Journal of Virology 64:1283–1289
    [Google Scholar]
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