1887

Abstract

The nucleotide sequence of the phosphoprotein (P) gene of a dolphin morbillivirus (DMV) isolate was determined. Like those of other morbilliviruses the DMV P gene encoded P and C proteins in overlapping open reading frames and V protein by editing the P gene transcript. Among P mRNA based clones the editing site variants GGGC, GGGG, GAGC and GGGGGGC predicting a P protein, and the variants GGGGC and GGGGG predicting a V protein, were found. Surprisingly, the three variants GGGC, GGGG and GAGC were also found among clones generated from genomic RNA of the DMV isolate. Thus, more than one viral genome type appeared to be present in cells infected with the DMV isolate. By a similar analysis of the virus genomes in the tissue from which the DMV isolate was obtained, only the GGGC type was found, indicating that the GGGG and GAGC types arose during adaptation of the virus to growth in cell cultures. No editing site variants likely to have arisen by editing the GAGC type were encountered, and it remains to be determined whether mRNA encoding V protein can be transcribed from genomes with this editing site. Using antisera raised against the common N terminus and unique C termini of the predicted P and V proteins, the expression of these proteins was demonstrated.

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1995-12-01
2022-07-06
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References

  1. Baron M. D., Shaila M. S., Barrett T. 1993; Cloning and sequence analysis of the phosphoprotein gene of rinderpest virus. Journal of General Virology 74:299–304
    [Google Scholar]
  2. Barrett T., Shrimpton S. B., Russell S. E. H. 1985; Nucleotide sequence of the entire protein coding region of canine distemper virus polymerase-associated (P) protein mRNA. Virus Research 3:367–372
    [Google Scholar]
  3. Barrett T., Visser I. K. G., Mamaev L., Goatley L., Van Bressem M.-F., Osterhaus A. D. M. E. 1993; Dolphin and porpoise morbilliviruses are genetically distinct from phocine distemper virus. Virology 193:1010–1012
    [Google Scholar]
  4. Bellini W. J., Englund G., Rozenblatt S., Arnheiter H., Richardson C. D. 1985; Measles virus P gene codes for two proteins. Journal of Virology 53:908–919
    [Google Scholar]
  5. Blixenkrone-Moller M. 1989; Detection of intracellular canine distemper virus antigen in mink inoculated with an attenuated or a virulent strain of canine distemper virus. American Journal of Veterinary Research 50:1616–1620
    [Google Scholar]
  6. Blixenkrone-Moller M., Sharma B., Varsanyi T. M., Hu A., Norrby E., Kovamees J. 1992; Sequence analysis of the genes encoding the nucleocapsid protein and phosphoprotein (P) of phocid distemper virus, and editing of the P gene transcript. Journal of General Virology 73:885–893
    [Google Scholar]
  7. Blixenkrone-Moller M., Bolt G., Gottschalck E., Renter M. 1994; Comparative analysis of the gene encoding the nucleocapsid protein of dolphin morbillivirus reveals its distant evolutionary relationship to measles virus and ruminant morbilliviruses. Journal of General Virology 75:2829–2834
    [Google Scholar]
  8. Bolt G., Blixenkrone-Moller M., Gottschalck E., Wishaupt R. G. A., Welsh M. J., Earle J. A. P., Rima B. K. 1994; Nucleotide and deduced amino acid sequences of the matrix (M) and fusion (F) protein genes of cetacean morbilliviruses isolated from a porpoise and a dolphin. Virus Research 34:291–304
    [Google Scholar]
  9. Brierley I., Digard P., Inglis S. C. 1989; Characterization of an efficient corona virus ribosomal frameshifting signal: requirement for an RNA pseudoknot. Cell 57:537–547
    [Google Scholar]
  10. Cattaneo R., Kaelin K., Baczko K., Billeter M. 1989; Measles virus editing provides an additional cysteine-rich protein. Cell 56:759–764
    [Google Scholar]
  11. Christensen J., Storgaard T., Bloch B., Alexandersen S., Aasted B. 1993; Expression of Aleutian mink disease parvovirus proteins in a baculovirus vector system. Journal of Virology 67:229–238
    [Google Scholar]
  12. Craigen W. J., Caskey C. T. 1987; Translational frameshifting: where will it stop?. Cell 50:1–2
    [Google Scholar]
  13. Curran J., Kolakofsky D. 1988; Ribosomal initiation from an ACG codon in the Sendai virus P/C mRNA. EM BO Journal 7:245–251
    [Google Scholar]
  14. Curran M. D., Rima B. K. 1992; The genes encoding the phospo- and matrix proteins of phocine distemper virus. Journal of General Virology 73:1587–1591
    [Google Scholar]
  15. Domingo M., Ferrer L., Pumarola M., Marco A. J., Plana J., Kennedy S., McAliskey M., Rima B. K. 1990; Morbillivirus in dolphins. Nature 348:21
    [Google Scholar]
  16. Galinski M. S., Troy R. M., Banerjee A. K. 1992; RNA editing in the phosphoprotein gene of the human parainfluenza virus type 3. Virology 186:543–550
    [Google Scholar]
  17. Gombart A. F., Hirano A., Wong T. 1992; Expression and properties of the V protein in acute measles virus and subacute sclerosing panencephalitis virus strains. Virus Research 25:63–78
    [Google Scholar]
  18. Kozak M. 1991; Structural features in eukaryotic mRNAs that modulate the initiation of translation. Journal of Biological Chemistry 266:19867–19870
    [Google Scholar]
  19. Lamb R. A., Paterson R. G. 1991; The nonstructural proteins of paramyxoviruses. In The Paramyxoviruses pp 181–214 Edited by Kingsbury D. W. New York: Plenum Press;
    [Google Scholar]
  20. Liston P., Briedis D. J. 1994; Measles virus V protein bind zinc. Virology 198:399–404
    [Google Scholar]
  21. Orvell C., Sheshberadaran H., Norrby E. 1985; Preparation and characterization of monoclonal antibodies directed against four structural components of canine distemper virus. Journal of General Virology 66:443–156
    [Google Scholar]
  22. Rima B. K., Cosby S. L., Duffy N., Lyons C., O’Loan D., Kennedy S., McCullough S. J., Smyth J. A., McNeilly F. 1990; Humoral immune responses in seals infected by phocine distemper virus. Research in Veterinary Science 49:114–116
    [Google Scholar]
  23. Ryan K. W., Morgan E. M., Portner A. 1991; Two non-contiguous regions of Sendai virus P protein combine to form a single nucleocapsid binding domain. Virology 180:126–134
    [Google Scholar]
  24. Sanger F., Nickelen S., Coulson A. R. 1977; DNA sequencing with chain terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  25. Sheshberadaran H., Norrby E., McCullough K. C., Carpenter W. C., Orvell C. 1986; The antigenic relationship between measles, canine distemper and rinderpest viruses studied with monoclonal antibodies. Journal of General Virology 67:1381–1392
    [Google Scholar]
  26. Sidhu M. S., Husar W., Cook S. D., Dowling P. C., Udem S. A. 1993; Canine distemper terminal and intergenic non-protein coding nucleotide sequences: completion of the entire CDV genome sequence. Virology 193:66–72
    [Google Scholar]
  27. Smallwood S., Ryan K. W., Moyer S. A. 1994; Deletion analysis defines a carboxyl-proximal region of Sendai virus P protein that binds to the polymerase L protein. Virology 202:154–163
    [Google Scholar]
  28. Thomas S. M., Lamb R. A., Paterson R. G. 1988; Two mRNAs that differ by two nontemplated nucleotides encode the amino coterminal proteins P and V of the paramyxovirus SV5. Cell 54:891–902
    [Google Scholar]
  29. Van Bressem M.-F., Visser I. K. G., De Swart R. L., Orvell C., Stanzani L., Androukaki E., Siakavara K., Osterhaus A. D. M. E. 1993; Dolphin morbillivirus in different parts of the Mediterranean Sea. Archives of Virology 129:235–242
    [Google Scholar]
  30. Visser I. K. G., Grachev M. A., Orvell C., DeVries P., Broeders H. W. J., Van De Bildt M. W. G., Groen J., Teppema J. S., Burger M. C., Uytdehaag F. G. C. M., Osterhaus A. D. M. E. 1990; Comparison of two morbilliviruses from seals during outbreaks of distemper in Northwest Europe and Siberia. Archives of Virology 111:149–164
    [Google Scholar]
  31. Wardrop E. A., Briedis D. J. 1991; Characterization of V protein in measles virus-infected cells. Journal of Virology 65:3421–3428
    [Google Scholar]
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