@article{mbs:/content/journal/jgv/10.1099/0022-1317-76-12-3039, author = "Pauly, T. and Elbers, K. and König, M. and Lengsfeld, T. and Saalmüller, A. and Thiel, H.-J.", title = "Classical swine fever virus-specific cytotoxic T lymphocytes and identification of a T cell epitope", journal= "Journal of General Virology", year = "1995", volume = "76", number = "12", pages = "3039-3049", doi = "https://doi.org/10.1099/0022-1317-76-12-3039", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-76-12-3039", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Classical swine fever virus (CSFV) -specific cytotoxic T lymphocytes (CTL) were derived from peripheral blood mononuclear leukocytes of immunized NIH-minipigs (MHC d/d haplotype) after in vitro restimulation with infectious CSFV. Their cytotoxic activity was determined against CSFV-infected target cells obtained from simian virus 40 (SV40) large T antigen-transfected immortalized kidney cells of a syngeneic miniature swine. Experiments with separated effector cell populations revealed that the CSFV-specific cytotoxic activity was mediated by CD4−CD6+CD8+ MHC class I-restricted T lymphocytes. Infection of target cells with various vaccinia virus/CSFV recombinants led to the identification of a major antigenic site for CSFV-specific CTL near the cleavage site between the non-structural proteins p80 (NS3) and p10 (NS4a). Using synthetic overlapping nonapeptides which covered this protein region the sequence ENALLVALF is the first sequence to be identified as an MHC class I-restricted T cell epitope recognized by CSFV-specific CTL.", }