1887

Abstract

In the process of generating an insertional mutant of herpesvirus of turkeys (HVT) expressing at the protein kinase (PK) locus, we isolated a recombinant which contained an intact PK gene but the short unique regions US1, US10 and SORF3 had been deleted and replaced by the cassette. Moreover, the virus contained duplicate copies of gD, gI and gE in an opposite orientation flanking , US2 and PK which were contiguous. These results are of interest in relation to the flexibility of the short unique segment (U) and of the inverted repeats flanking U of the alpha-herpesviruses. The recombinant expressed β-galactosidase and was genetically stable and . Chickens inoculated with the virus developed antibodies to HVT antigens and to β-galactosidase but the replication of the recombinant was impaired in comparison to parental HVT as shown by a reduction in the proportion of infected lymphocytes.

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/content/journal/jgv/10.1099/0022-1317-76-11-2903
1995-11-01
2022-05-28
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References

  1. Brunovskis P., Velicer L. F. 1995; The Marek’s disease virus (MDV) unique short region: alphaherpesvirus-homologous, fowlpox virus-homologous and MDV-specific genes. Virology 206:324–338
    [Google Scholar]
  2. Davison A. J., McGeoch D. J. 1986; Evolutionary comparisons of the S segments in the genomes of herpes simplex virus type 1 and varicella-zoster virus. Journal of General Virology 67:597–611
    [Google Scholar]
  3. Morgan R. W., Gelb J., Schreurs CH. S., Lütticken D., Rosenberger J. K., Sondermeijer P. J. A. 1992; Protection of chickens from Newcastle and Marek’s diseases with a recombinant herpesvirus of turkeys vaccine expressing the Newcastle disease virus fusion protein. Avian Diseases 36:858–870
    [Google Scholar]
  4. Ross L. J. N., Binns M. M., Tyers P., Pastorek J., Zelník V., Scott S. 1993; Construction and properties of a turkey herpesvirus recombinant expressing the Marek’s disease virus homologue of glycoprotein B of herpes simplex virus. Journal of General Virology 74:371–377
    [Google Scholar]
  5. Sakaguchi M., Urakawa T., Hirayama Y., Miki N., Yamamoto M., Hirai K. 1992; Sequence determination and genetic content of an 8.9 kbp restriction fragment in the short unique region and the internal inverted repeat of Marek’s disease virus type 1 DNA. Virus Genes 6:365–378
    [Google Scholar]
  6. Sakaguchi M., Urakawa T., Hirayama Y., Miki N., Yamamoto M., Zhu G.S., Hirai K. 1993; Marek’s disease virus protein kinase gene identified within the short unique region of the viral genome is not essential for viral replication in cell culture and vaccine-induced immunity in chickens. Virology 195:140–148
    [Google Scholar]
  7. Witter R. L., Nazerian K., Purchase H. G., Burgoyne G. H. 1970; Isolation from turkeys of a cell–associated herpesvirus antigenically related to Marek’s disease virus. American Journal of Veterinary Research 31:525–538
    [Google Scholar]
  8. Zelník V., Darteil R., Audonnet J. C., Smith G. D., Riviere M., Pastorek J., Ross L. J. N. 1993; The complete sequence and gene organization of the short unique region of herpesvirus of turkeys. Journal of General Virology 74:2151–2162
    [Google Scholar]
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