Pseudorabies virus early protein 0 transactivates the viral gene promoters Free

Abstract

Pseudorabies virus (PRV) early protein 0 (EP0) contains the RING finger domain with homology to the immediate-early (IE) protein ICP0 of herpes simplex virus type 1 (HSV-1). EP0 was detected by indirect immunofluorescence in the nuclei of the cells transfected with EP0 expression plasmid as is the case in cells infected with PRV. In transient expression assays, EP0 trans-activated the PRV IE, thymidine kinase (TK) and glycoprotein X (gX) promoters, indicating that EP0, like ICP0 of HSV-1, is a transactivating protein.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-11-2881
1995-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/11/JV0760112881.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-11-2881&mimeType=html&fmt=ahah

References

  1. Cheung A. K. 1991; Cloning of the latency gene and the early protein 0 gene of pseudorabies virus. Journal of Virology 65:5260–5271
    [Google Scholar]
  2. Everett R. D. 1988; Analysis of the functional domains of herpes simplex virus type 1 immediate-early polypeptide VmwllO. Journal of Molecular Biology 202:87–96
    [Google Scholar]
  3. Everett R. D., Barlow P., Milner A., Luisi B., Orr A., Hope G., Lyon D. 1993a; A novel arrangement of zinc-binding residues and secondary structure in the C3HC4 motif of an alphaherpes virus protein family. Journal of Molecular Biology 234:1038–1047
    [Google Scholar]
  4. Everett R. D., Cross A., Orr A. 1993b; A truncated form of herpes simplex virus type 1 immediate-early protein VmwllO is expressed in a cell type dependent manner. Virology 197:751–756
    [Google Scholar]
  5. Fraefel C., Zeng J., Choffat Y., Engels M., Schwyzer M., Ackermann M. 1994; Identification and zinc dependence of the bovine herpesvirus 1 transactivator protein BICPO. Journal of Virology 68:3154–3162
    [Google Scholar]
  6. Francki R. I. B., Fauquet C. M., Knudson D. L., Brown F. 1991; Classification and Nomenclature of Viruses. Fifth Report of the International Committee on Taxonomy of Viruses. Archives of Virology Supplementum 2
    [Google Scholar]
  7. Freemont P. S. 1993; The RING finger. A novel protein sequence motif related to the zinc finger. Annals of the New York Academy of Sciences 684:174–192
    [Google Scholar]
  8. Greaves R., O’Hare P. 1989; Separation of requirements for protein-DNA complex assembly from those for functional activity in the herpes simplex virus regulatory protein Vmw65. Journal of Virology 63:1641–1650
    [Google Scholar]
  9. Mitchell P. J., Tjian R. 1989; Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins. Science 245:371–378
    [Google Scholar]
  10. Moriuchi H., Moriuchi M., Smith H. A., Straus S. E., Cohen J. I. 1992; Varicella-zoster virus open reading frame 61 protein is functionally homologous to herpes simplex virus type 1 ICPO. Journal of Virology 66:7303–7308
    [Google Scholar]
  11. Moriuchi H., Moriuchi M., Straus S. E., Cohen J. I. 1993; Varicella-zoster virus (VZV) open reading frame 61 protein trans- activates VZV gene promoters and enhances the infectivity of VZV DNA. Journal of Virology 67:4290–4295
    [Google Scholar]
  12. Moriuchi H., Moriuchi M., Cohen J. I. 1994; The RING finger domain of the varicella-zoster virus open reading frame 61 protein is required for its transregulatory functions. Virology 205:238–246
    [Google Scholar]
  13. Nagpal S., Ostrove J. M. 1991; Characterization of a potent varicella-zoster virus-encoded trans-repressor. Journal of Virology 65:5289–5296
    [Google Scholar]
  14. Nilsson B., Abrahmsen L., Uhlen M. 1985; Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors. EMBO Journal 4:1075–1080
    [Google Scholar]
  15. O’Hare P., Hayward G. S. 1985; Three trans-acting regulatory proteins of herpes simplex virus modulate immediate-early gene expression in a pathway involving positive and negative feedback regulation. Journal of Virology 56:723–733
    [Google Scholar]
  16. Patwardhan S., Gupta K. C. 1988; Translation initiation potential of the 5′ proximal AUGs of the polycistronic P/C mRNA of Sendai virus. A multipurpose vector for site-specific mutagenesis. Journal of Biological Chemistry 263:4907–4913
    [Google Scholar]
  17. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  18. Taharaguchi S., Inoue H., Ono E., Kida H., Yamada S., Shimizu Y. 1994; Mapping of transcriptional regulatory domains of pseudorabies virus immediate-early protein. Archives of Virology 137:289–302
    [Google Scholar]
  19. Vlček C., Kozmík Z., Pačes V., Schirm S., Schwyzer M. 1990; Pseudorabies virus immediate-early gene overlaps with an oppositely oriented open reading frame: characterization of their promoter and enhancer regions. Virology 179:365–377
    [Google Scholar]
  20. Wirth U. V., Fraefel C., Vogt B., Vlcek C., Paces V., Schwyzer M. 1992; Immediate-early RNA 2.9 and early RNA 2.6 of bovine herpesvirus 1 are 3′ coterminal and encode a putative zinc finger transactivator protein. Journal of Virology 66:2763–2772
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-11-2881
Loading
/content/journal/jgv/10.1099/0022-1317-76-11-2881
Loading

Data & Media loading...

Most cited Most Cited RSS feed