1887

Abstract

Algal virus infection proceeds via the specific recognition of the host cell wall, penetration of the cell wall and transfer of genetic material into the cytoplasm of the host cell. This process is similar to that which occurs when bacteriophage infect bacteria so that techniques and concepts developed to study bacteriophage are applicable to algal virus studies. By measuring virus-induced changes in chlorophyll fluorescence we have redefined classical studies on the distribution of infectivity. We show that infectivity does not follow a Poisson distribution with a fixed mean, . By analysing the infectivity of algal viruses over a broad range of virus:cell ratios we have obtained a corrected Poisson distribution that reflects the probability of multiple virus particles attached per cell and is equally applicable to algal viruses and bacteriophage.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-11-2859
1995-11-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/11/JV0760112859.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-11-2859&mimeType=html&fmt=ahah

References

  1. Delbrück M., Luria S. E. 1943; Interference between bacterial viruses. I. Interference between two bacterial viruses upon the same host, and the mechanism of virus growth. Archives of Biochemistry 1:111–141
    [Google Scholar]
  2. Ellis E. L., Delbruck M. 1939; The growth of the bacteriophage. Journal of General Physiology 22:365–384
    [Google Scholar]
  3. Girton L. E., Van Etten J. L. 1987; Restriction site map of the Chlorella virus PBCV-1 genome. Plant Molecular Biology 9:247–257
    [Google Scholar]
  4. Meints R. H., Burbank D. E., Van Etten J. L., Lamport D. T. A. 1983; Properties of the Chlorella receptor for the virus PBCV-1. Virology 164:15–21
    [Google Scholar]
  5. Neter J., Wasserman W., Kutner M. H. 1985 Applied Linear Statistical Models 2nd edn., pp 160–165 Illinois: Richard D. Irwin;
    [Google Scholar]
  6. Rohozinski J., Girton L. E., Van Etten J. L. 1989; Chlorella virus contain linear non-permuted double-stranded DNA genomes with covalently-closed hairpin ends. Virology 168:363–369
    [Google Scholar]
  7. Schreiber U. 1986; Detection of rapid induction kinetics with a new type of high frequency modulated chlorophyll fluorimeter. Photosynthesis Research 9:261–272
    [Google Scholar]
  8. Schreiber U., Schliwa U., Bilger W. 1986; Continuous recording of photochemical and non-photochemical fluorescence quenching with a new type of modulation fluorimeter. Photosynthesis Research 10:51–62
    [Google Scholar]
  9. Seaton G. G. R., Lee K., Rohozinski J. 1995; Photosynthetic shutdown in Chlorella NC64A associated with the infection cycle of the virus PBCV-1. Plant Physiology 108: (in press)
    [Google Scholar]
  10. Seaton G. G. R., Walker D. A. 1992; Validating chlorophyll fluorescence measures of efficiency: observations on fluorimetnc estimation of photosynthetic rate. Proceedings of the Royal Society of London series B 249:41–47
    [Google Scholar]
  11. Stent G. S. 1963; Growth cycle. In Molecular Biology of Bacterial Viruses pp 70–87 San Francisco: W. H Freeman;
    [Google Scholar]
  12. Van Etten J. L., Meints R. H., Kuczmarski D., Burbank D. E., Lee K. 1982; Viruses of symbiotic Chlorella-like algae isolated from Paramecium bursaria and Hydra viridis. Proceedings of the National Academy of Sciences, USA 79:3867–3871
    [Google Scholar]
  13. Van Etten J. L., Burbank D. E., Kuczmarski D., Meints R. H. 1983; Virus infection of culturable Chlorella-like alga and development of a plaque assay. Science 219:994–996
    [Google Scholar]
  14. Van Etten J. L., Lane L. C., Meints R. H. 1991; Viruses and virus-like particles of eukaryotic algae. Microbiological Reviews 55:586–620
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-11-2859
Loading
/content/journal/jgv/10.1099/0022-1317-76-11-2859
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error