1887

Abstract

We examined the genetic and antigenic properties of Dobrava (DOB) virus, a hantavirus associated with severe haemorrhagic fever with renal syndrome in Europe. Cloning and sequence analyses revealed the DOB M segment to consist of 3644 nucleotides, with a coding capacity of 1134 amino acids in the virus complementary-sense RNA (cRNA). Seven potential asparagine-linked glycosylation sites were identified in the M segment gene product, one in the G2 and six in the G1 coding regions. The S segment is 1667 nucleotides long, and has a single ORF in the cRNA capable of encoding a protein of 428 amino acids. Phylogenetic comparisons of the M and S segments of DOB virus to those of other hantaviruses indicated that DOB virus is similar to, but clearly distinct from Hantaan (HTN) and Seoul (SEO) viruses. Certain G2-specific, but not G1-specific monoclonal antibodies to HTN virus reacted to the same titre with DOB and homologous viral antigen. Plaque-reduction neutralization tests indicated that, of the sera tested, only antisera to SEO virus were able to neutralize DOB virus to a titre greater than 1:10; however, this neutralization titre was eightfold lower than that observed with homologous SEO virus. The data reported here confirm that DOB virus is a unique species in the genus, family .

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-11-2801
1995-11-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/11/JV0760112801.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-11-2801&mimeType=html&fmt=ahah

References

  1. Arikawa J., Schmaljohn A. L., Dalrymple J. M., Schmaljohn C. S. 1989; Characterization of Hantaan virus envelope glyco protein antigenic determinants defined by monoclonal antibodies. Journal of General Virology 70:615–624
    [Google Scholar]
  2. Arikawa J., Connors L. L., Wang M., Schmaljohn C. S. 1990; Coding properties of the S and the M genome segments of Sapporo rat virus: comparison to other causative agents of hemorrhagic fever with renal syndrome. Virology 176:114–125
    [Google Scholar]
  3. Avsic-Zupanc T., Poljak M. 1994; Hantavirus genomic variation and disease distribution. Zdravstveni Vestnik 63: (Suppl 2) 25–27
    [Google Scholar]
  4. Avsic-Zupanc T., Likar M., Novakovic S., Cizman B., Kraigher A., Van der Groen G., Stojanovic R., Gligic A., LeDuc J. 1990; Evidence of the presence of two hantaviruses in Slovenia, Yugoslavia. Archives of Virology 115:87–94
    [Google Scholar]
  5. Avsic-Zupanc T., Xiao S. Y., Stojanovic R., Gligic A., Vander Groen G., LeDuc J. W. 1992; Characterization of Dobrava virus: a hantavirus from Slovenia, Yugoslavia. Journal of Medical Virology 38:132–137
    [Google Scholar]
  6. Avsic-Zupanc T., Poljak M., Furlan P., Kaps R., Xiao S. Y., Leduc J. W. 1994; Isolation of a strain of a Hantaan virus from a fatal case of hemorrhagic fever with renal syndrome in Slovenia. American Journal of Tropical Medicine and Hygiene 51:393–400
    [Google Scholar]
  7. Carey D., Reuben R., Panicker K., Shope R., Myers R. 1971; Thottapalayam virus: a presumptive arbovirus isolated from a shrew in India. Indian Journal of Medical Research 59:1758–1760
    [Google Scholar]
  8. Childs J., Ksiazek T., Spiropoulou C., Krebs J., Morzunov S., Maupin G., Gage K., Rollin P., Sarisky J., Enscore R., Frey J., Peters C., Nichol S. 1994; Serologic and genetic identification of Peromyscus maniculatus as the primary rodent reservoir for a new hantavirus in the southwestern United States. Journal of Infectious Diseases 169:1271–1280
    [Google Scholar]
  9. Chu Y.-K., Rossi C., LeDuc J., Lee H., Schmaljohn C., Dalrymple J. 1994; Serological relationships among viruses in the Hantavirus genus, family Bunyaviridae . Virology 198:196–204
    [Google Scholar]
  10. Elliott L. H., Ksiazek T. G., Rollin P. E., Spiropoulou C. F., Morzunov S., Monroe M., Goldsmith C. S., Humphrey C. D., Zaki S. R., Krebs J. W. J. W., Maupin G., Gage K., Childs J. E., Nichol S. T., Peters C. J. 1994; Isolation of the causative agent of hantavirus pulmonary syndrome. American Journal of Tropical Medicine and Hygiene 51:102–108
    [Google Scholar]
  11. Giebel L., Stohwasser R., Zöller L., Bautz E., Darai G. 1989; Determination of coding capacity of the M genome segment of nephropathia epidemica virus strain Hällnäs B1 by molecular cloning and nucleotide sequence analysis. Virology 172:498–505
    [Google Scholar]
  12. Gligic A., Dimkovic N., Xiao S., Buckle G., Jovanovic D., Velimirovic D., Stojanovic R., Obradovic M., Diglisic G., Micic J., Asher D., LeDuc J., Yanagihara R., Gasekjdu D. 1992; Belgrade virus: a new hantavirus causing severe hemorrhagic fever with renal syndrome in Yugoslavia. Journal of Infectious Diseases 166:113–120
    [Google Scholar]
  13. Hjelle B., Chavez-Giles F., Torrez-Martinez N., Yates T., Sarisky J., Webb J., Ascher M. 1994; Genetic identification of a novel hantavirus of the harvest mouse Reithrodontomys megalotis . Journal of Virology 68:6751–6754
    [Google Scholar]
  14. Lee H. 1982; Korean hemorrhagic fever. Progress in Medical Virology 28:96–113
    [Google Scholar]
  15. Lee H., Vander Groen G. 1989; Hemorrhagic fever with renal syndrome. Progress in Medical Virology 36:62–102
    [Google Scholar]
  16. Morzunov S. P., Feldmann H., Spiropoulou C. F., Semenova V. A., Rollin P. E., Ksiazek T. G., Peters C. J., Nichol S. T. 1995; A newly recognized virus associated with a fatal case of hantavirus pulmonary syndrome in Louisiana. Journal of Virology 69:1980–1983
    [Google Scholar]
  17. Parrington M., Kang C. 1990; Nucleotide sequence analysis of the S genomic segment of Prospect Hill virus comparison with the prototype hantavirus. Virology 175:167–175
    [Google Scholar]
  18. Parrington M., Lee P., Kang C. 1991; Molecular characterization of the Prospect Hill virus M RNA segment a comparison with the M RNA segments of other hantaviruses. Journal of General Virology 72:1845–1854
    [Google Scholar]
  19. Plyusnin A., Vapalahti O., Lankinen H., Lehvaslaiho H., Apekina N., Myasnikov Y., Kallio-Kokko H., Henttonen H., Lundkvist A., Brummer-Korvenkontio M., Vaheri A. 1994; Tula virus: a newly detected hantavirus carried by European common voles. Journal of Virology 68:7833–7839
    [Google Scholar]
  20. Puthavathana P., Lee H., Kang C. 1992; Typing of hantaviruses from five continents by polymerase chain reaction. Virus Research 26:1–13
    [Google Scholar]
  21. Rollin P. E., Ksiazek T. G., Elliott L. H., Ravkov E. V., Morzunov S., Livingstone W., Monroe M., Glass G., Ruo S., Khan A., Childs J., Nichol S. T., Peters C. J. 1995; Isolation of Black Creek Canal virus, a new hantavirus from Sigmodon hispidus in Florida. Journal of Medical Virology 46:35–39
    [Google Scholar]
  22. Schmaljohn C. S. 1995; The molecular biology of hantaviruses. In The Bunyaviridae Edited by Elliott R. M. New York: Plenum Press; (in press)
    [Google Scholar]
  23. Schmaljohn C. S., Hasty S. E., Harrison S. A., Dalrymple J. M. 1983; Characterization of Hantaan virions, the prototype virus of hemorrhagic fever with renal syndrome. Journal of Infectious Diseases 148:1005–1012
    [Google Scholar]
  24. Schmaljohn C., Hasty S., Dalrymple J., LeDuc J., Lee H., Von Bonsdorff C.-H., Brummer-Korvenkontio M., Vaheri A., Tsai T., Regnery H., Goldgaber D., Lee P.-W. 1985; Antigenic and genetic properties of viruses linked to hemorrhagic fever with renal syndrome. Science 227:1041–1044
    [Google Scholar]
  25. Schmaljohn C., Jennings C., Hay J., Dalrymple J. 1986; Coding strategy of the S genome of Hantaan virus. Virology 155:633–643
    [Google Scholar]
  26. Schmaljohn C. S., Schmaljohn A. L., Dalrymple J. M. 1987; Hantaan virus M RNA: coding strategy, nucleotide sequence, and gene order. Virology 157:31–39
    [Google Scholar]
  27. Schmaljohn A., Li D., Negley D., Bressler D., Turell M., Korch G., Ascher M., Schmaljohn C. 1995; Isolation and initial characterization of a new hantavirus from California. Virology 206:963–972
    [Google Scholar]
  28. Spiropoulou C. F., Morzunov S., Feldmann H., Sanchez A., Peters C. J., Nichol S. T. 1994; Genome structure and variability of a virus causing hantavirus pulmonary syndrome. Virology 200:715–723
    [Google Scholar]
  29. Stohwasser R., Giebel L., Zoeller L., Bautz E., Darai G. 1990; Molecular characterization of the RNA S segment of nephropathia epidemica virus strain Hallnas Bl. Virology 174:79–86
    [Google Scholar]
  30. Torrez-Martinez N., Song W., Hjelle B. 1995; Nucleotide sequence analysis of the M genomic segment of El Moro Canyon hantavirus: antigenic distinction from Four Corners hantavirus. Virology 211:336–338
    [Google Scholar]
  31. Turell M., Korch G., Rossi C., Sesline D., Enge B., Dondero D., Jay M., Ludwig G., Li D., Schmaljohn C., Jackson R., Ascher M. 1995; Prevalence of hantaviral infection in rodents associated with two fatal human infections in California. American Journal of Tropical Medicine and Hygiene 52:180–182
    [Google Scholar]
  32. Vander Groen G. 1990; Hantavirus variation and disease distribution. In Applied Virology Research vol 2 Virus Variability, Epidemiology and Control pp 317–334 Edited by Kurstak E., Marusky R. G., Murphy F. A., Regenmortel M. V. New York: Plenum Press;
    [Google Scholar]
  33. Xiao S. Y., Diglisic G., Avsic-Zupanc T., LeDuc J. W. 1993; Dobrava virus is a new Hantavirus: evidenced by comparative sequence analysis. Journal of Medical Virology 39:152–155
    [Google Scholar]
  34. Xiao S. Y., LeDuc J. W., Chu Y. K., Schmaljohn C. S. 1994; Phylogenetic analyses of virus isolates m the genus Hantavirus, family Bunyaviridae . Virology 198:205–21
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-11-2801
Loading
/content/journal/jgv/10.1099/0022-1317-76-11-2801
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error