1887

Abstract

CD46 downregulation by measles virus (MV) occurs after expression of virus haemagglutinin (H) protein on the surface of the infected cell and is a consequence of CD46-H interaction on the cell membrane. To assess whether CD46 downregulation also occurs after CD46-H interaction when these two molecules are expressed on distinct cells, we used human T cell line Jurkat (expressing CD46) and transfected murine fibroblast line L stably expressing MV-H protein (L.H). FACS analysis shows that cell-to-cell contact of 1 h at 37 °C triggers a reduction of CD46 cell surface labelling as detected by MCI20.6, GB24 and J4-48 monoclonal antibodies. This reduction is similar to that observed after MV infection or after infection with recombinant vaccinia virus encoding MV-H protein. By contrast, MV-H protein was downregulated only when CD46-H interaction occurred on the same cell membrane. CD46 downregulation is specific for CD46-H interaction because it was not observed after coincubation of Jurkat cells with either L cells expressing MV nucleoprotein (L.NP) or L cells. Moreover, this downregulation could be blocked by either anti-CD46 or anti-H antibodies. The H-mediated CD46 downregulation is reversible and restricted to CD46 since expression of other surface markers (CD3, CD14, CD47 and CD63) is unaffected. It is apparently not mediated in a protein kinase (PK) A- or PKC-dependent manner. Altogether, our results provide an unequivocal demonstration that interaction between the extracellular domains of CD46 and MV-H is sufficient to trigger CD46 downregulation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-11-2793
1995-11-01
2022-01-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/11/JV0760112793.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-11-2793&mimeType=html&fmt=ahah

References

  1. Bihoreau C., Heurtier A., Enjalbert A., Corvaia N., Bensussan A., Degos L., Kordon C. 1991; Activation of the CD3/T cell receptor(TcR) complex or of protein kinase C potentiates adenylyl cyclase stimulation in tumoral T cell line: involvement of two distinct intracellular pathways. European Journal of Immunology 21:2877–2882
    [Google Scholar]
  2. Bloom B. R. 1989; Vaccines for the third world. Nature 342:115–120
    [Google Scholar]
  3. Cho S. W., Oglesby T. J., Hsi B. L., Adams E. M., Atkinson J. P. 1991; Characterization of three monoclonal antibodies to membrane cofactor protein (MCP) of the complement system and quantification of MCP by radioassay. Clinical and Experimental Immunology 83:257–261
    [Google Scholar]
  4. Dietrich J., Hou X., Wegener A.-M. K., Eisler C. 1994; CD3Γ contains a phosphoserine-dependent di-leucine motif involved in downregulation of the T cell receptor. EMBO Journal 13:2156–2166
    [Google Scholar]
  5. Dorig R. E., Marcil A., Chopra A., Richardson C. D. 1993; The human CD46 molecule is a receptor for measles virus (Edmonston strain). Cell 75:295–305
    [Google Scholar]
  6. Dohlman H. G., Thorner J., Caron M. G., Lefkovitz R. J. 1991; Model system for the study of seven-transmembrane-segment receptors. Annual Review of Biochemistry 60:653–688
    [Google Scholar]
  7. Gerlier D., Trescol-Biemont M. C., Varior-Krishnan G., Naniche D., Fugier-Vivier I., Rabourdin-Combe C. 1994a; Efficient MHC class II-restricted presentation of measles virus relies on haemagglutinin-mediated targeting to its cellular receptor human CD46 expressed by murine B cells. Journal of Experimental Medicine 179:353–358
    [Google Scholar]
  8. Gerlier D., Loveland B., Varior-Krishnan G., Thorley B., McKenzie I. F. C., Rabourdin-Combe C. 1994b; Measles virus receptor properties are shared by several CD46 isoforms differing in extracellular regions and cytoplasmic tails. Journal of General Virology 75:2163–2171
    [Google Scholar]
  9. Giraudon P., Wild T. F. 1985; Correlation between epitopes on hemagglutinin of measles virus and biological activities: passive protection by monoclonal antibodies is related to their hemagglutinin inhibiting activity. Virology 144:46–58
    [Google Scholar]
  10. Gschwendt M., Kittstein W., Marks F. 1991; Protein kinase C activation by phorbol esters: do cysteine-rich regions and pseudosubstrate motifs play a role?. Trends in Biochemical Science 16:167–169
    [Google Scholar]
  11. Lee N. H., Fraser C. M. 1993; Cross-talk between m1 muscarinic acetylcholine and β2-adrenergic receptors. Journal of Biological Chemistry 268:7949–7957
    [Google Scholar]
  12. Lee J. D., Kravachenko V., Kirkland T. N., Han J., Mackman N., Moriarty A., Leturcq D., Tobias P. S., Ulevitch R. J. 1993; Glycosyl-phosphatidylinositol-anchored or integral membrane forms of CD14 mediate identical cellular responses to endotoxin. Proceedings of Natlional Academy of Sciences USA 90:9930–9934
    [Google Scholar]
  13. Liszewski M. K., Post T. W., Atkinson J. P. 1991; Membrane cofactor protein (MCP or CD46): newest member of the regulators of complement activation gene cluster. Annual Review of Immunology 9:431–455
    [Google Scholar]
  14. Lombard-platet S., Bertolino P., Gimenez C., Humbert M., Gerlier D., Rabourdin-Combe C. 1993; Invariant chain expression similarly controls presentation of endogenously synthesized and exogenous antigens by MHC class II molecules. Cellular Immunology 148:60–70
    [Google Scholar]
  15. Mcchesney M. B., Kehrl J. H., Valsmakis A., Fauci A. S., Oldstone M. B. A. 1987; Measles virus infection of B lymphocytes permits cellular activation but blocks progression through the cell cycle. Journal of Virology 61:3441–3447
    [Google Scholar]
  16. Mcchesney M. B., Altman A., Oldstone M. B. A. 1988; Supression of T lymphocyte function by measles virus is due to cell cycle arrest in Gl. Journal of Immunology 140:1269–1273
    [Google Scholar]
  17. Maisner A., Schneider-Schaulies J., Liszewski M. K., Atkinson J. P., Herrler G. 1994; Binding of measles virus to membrane cofactor protein (CD46): importance of disulfide bonds and N-glycans for the receptor function. Journal of Virology 68:6299–6304
    [Google Scholar]
  18. Manchester M., Liszewski M. K., Atkinson J. P., Oldstone M. B. A. 1994; Multiple isoforms of CD46 (membrane cofactor protein) serve as receptors for measles virus. Proceedings of National Academy of Sciences, USA 91:2161–2165
    [Google Scholar]
  19. Morrison P., Takashima K., Risch Rosner M. 1993; Role of threonine residues in regulation of the epidermal growth factor receptor by protein kinase C and mitogen-activated protein kinase. Journal of Biological Chemistry 268:15536–15543
    [Google Scholar]
  20. Naniche D., Wild T. F., Rabourdin-Combe C., Gerlier D. 1992; A monoclonal antibody recognizes a human cell surface glycoprotein involved in measles virus binding. Journal of General Virology 73:2167–2624
    [Google Scholar]
  21. Naniche D., Varior-Krishnan G., Cervoni F., Wild T. F., Rossi B., Rabourdin-Combe C., Gerlier D. 1993a; Human membrane cofactor protein (CD46) acts as a cellular receptor for measles virus. Journal of Virology 67:6025–6032
    [Google Scholar]
  22. Naniche D., Wild T. F., Rabourdin-Combe C., Gerlier D. 1993b; Measles virus haemagglutinin induces down-regulation of gp57/67, a molecule involved in virus binding. Journal of General Virology 74:1073–1079
    [Google Scholar]
  23. Norrby E., Oxman M. N. 1990; Measles virus. In Virology pp 1013–1044 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  24. Richardson C. D., Choppin P. W. 1983; Oligopeptides that specifically inhibit membrane fusion by paramyxoviruses: studies on the site of action. Virology 131:518–532
    [Google Scholar]
  25. Ross A., Rapuano M., Prives J. 1988; Induction of phosphorylation and cell surface redistribution of acetylcholine receptors by phorbol ester and carbamylcholine in cultured chick muscle cells. Journal of Cell Biology 107:1139–1145
    [Google Scholar]
  26. Rothenberger S., Iacopetta B. J., Kuhn L. C. 1987; Endo-cytosis of the transferrin receptor requires the cytoplasmic domain but not its phosphorylation site. Cell 49:423–431
    [Google Scholar]
  27. Russel S. M., Loveland B. E., Johnstone R. W., Thorley B. R., Mckenzie I. F. C. 1992; Functional characterization of alternatively spliced CD46 cytoplasmic tails. Transplantation Proceedings 24:2329–2330
    [Google Scholar]
  28. Schnorr J. J., Dunster L. M., Nanan R., Schneider-Schaulies J., Schneider-Schaulies S., Ter Meulen V. 1995; Measles virus induced downregulation of CD46 is associated with enhanced sensitivity to complement-mediated lysis of infected cells. European Journal of Immunology 25:976–985
    [Google Scholar]
  29. Yanagi Y., Cubitt B. A., Oldstone M. A. 1992; Measles virus inhibits mitogen-induced T cell proliferation but does not directly perturb the T cell activation process inside the cell. Virology 187:280–289
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-11-2793
Loading
/content/journal/jgv/10.1099/0022-1317-76-11-2793
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error