%0 Journal Article %A Wang, Eryu %A Ryman, Kate D. %A Jennings, Alan D. %A Wood, David J. %A Taffs, Frank %A Minor, Philip D. %A Sanders, Peter G. %A Barrett, Alan D. T. %T Comparison of the genomes of the wild-type French viscerotropic strain of yellow fever virus with its vaccine derivative French neurotropic vaccine %D 1995 %J Journal of General Virology, %V 76 %N 11 %P 2749-2755 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-76-11-2749 %I Microbiology Society, %X The French neurotropic vaccine, or FNV, was used extensively in Africa to control yellow fever (YF). Although efficacious, the vaccine caused an unacceptable rate of post-vaccinal complications in children and was subsequently replaced by the 17D vaccine. Here we report that the genomes of the wild-type YF virus French viscerotropic virus and its attenuated vaccine derivative, FNV virus from the Institut Pasteur, Paris, (FNV-IP) differ by 77 nucleotides encoding 35 amino acid substitutions. Comparison of FNV-IP and three other isolates of FNV with other YF vaccine strains (17D-204 and 17DD derived from wild-type strain Asibi) revealed that during the two attenuation processes two common nucleotide changes arose that encode two amino acid substitutions: one is in the membrane protein at amino acid 35 (M-35), the other in nonstructural (NS) protein 4B at NS4B-95. These common substitutions may be important in the process of attenuation of viscerotropic disease for humans and monkeys, and/or may be involved in loss of mosquito competence of the vaccine viruses. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-76-11-2749