1887

Abstract

We evaluated the function of human immunodeficiency virus type 1 gene in various cell lines. We established a highly sensitive system consisting of chloramphenicol acetyltransferase and reverse transcriptase assays and used it to monitor the effects of mutation of the gene. In some cell lines, Vpu protein was not required at the early phase of viral replication but was important for efficient virion production. In these cells, the Vpu protein functioned effectively irrespective of the presence of intact gene products. Likewise, CD4 gene expression had no effects on Vpu function. In the other cell lines tested, Vpu protein was not important for virion release, and the mutant clone generated a normal level of progeny virions upon transfection.

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1995-11-01
2021-10-18
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References

  1. Adachi A., Gendelman H. E., Koenig S., Folks T., Willey R., Rabson A., Martin M. A. 1986; Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone. Journal of Virology 59:284–291
    [Google Scholar]
  2. Adachi A., Ono N., Sakai H., Ogawa K., Shibata R., Kiyomasu T., Masuike M., Ueda S. 1991; Generation and characterization of the human immunodeficiency virus type 1 mutants. Archives of Virology 117:45–58
    [Google Scholar]
  3. Arrigo S. J., Chen I. S. Y. 1991; Rev is necessary for translation but not cytoplasmic accumulation of HIV-1. vif, vpr, and env/vpu 2 RNAs. Genes & Development 5:808–819
    [Google Scholar]
  4. Chesebro B., Wehrly K. 1988; Development of a sensitive quantitative focal assay for human immunodeficiency virus in-fectivity. Journal of Virology 62:3779–3788
    [Google Scholar]
  5. Cohen E. A., Terwilliger E. F., Sodroski J. G., Haseltine W. A. 1988; Identification of a protein encoded by the vpu gene of HIV-1. Nature 334:532–534
    [Google Scholar]
  6. Cullen B. R., Greene W. C. 1990; Functions of the auxiliary gene products of the human immunodeficiency virus type 1. Virology 178:1–5
    [Google Scholar]
  7. Folks T., Benn S., Rabson A., Theodore T., Hoggan M. D., Martin M., Lightfoote M., Sell K. 1985; Characterization of a continuous T-cell line susceptible to the cytopathic effects of the acquired immune deficiency syndrome (AIDS)-associated retrovirus. Proceedings of the National Academy of Sciences, USA 82:4539–4543
    [Google Scholar]
  8. Freed E. O., Myers D. J., Risser R. 1989; Mutational analysis of the cleavage sequence of the human immunodeficiency virus type 1 envelope glycoprotein precursor gpl60. Journal of Virology 63:4670–4675
    [Google Scholar]
  9. Gorman C. M., Moffat L. F., Howard G. H. 1982; Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Molecular and Cellular Biology 2:1033–1051
    [Google Scholar]
  10. Graham F. L., van Der Eb A. J. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–467
    [Google Scholar]
  11. Helseth E., Kowalski M., Gabuzda D., Olshevsky U., Haseltine W., Sodroski J. 1990; Rapid complementation assays measuring replicative potential of human immunodeficiency virus type 1 envelope glycoprotein mutant. Journal of Virology 64:2416–2420
    [Google Scholar]
  12. Klimkait T., Strebel K., Hoggan M. D., Martin M. A., Orenstein J. M. 1990; The human immunodeficiency virus type 1-specific protein vpu is required for efficient virus maturation and release. Journal of Virology 64:621–629
    [Google Scholar]
  13. Koenig S., Hirsch V. M., Olmsted R. A., Powell D., Maury W., Rabson A., Fauci A. S., Purcell R. H., Johnson P. R. 1989; Selective infection of human CD4+ cells by simian immunodeficiency virus: productive infection associated with envelope glycoprotein-induced fusion. Proceedings of the National Academy of Sciences, USA 86:2443–2447
    [Google Scholar]
  14. Matsuda Z., Chou M.-J., Matsuda M., Huang J.-H., Chen Y.-M., Redfield R., Mayer K., Essex M., Lee T. H. 1988; Human immunodeficiency virus type 1 has an additional coding sequence in the central region of the genome. Proceedings of the National Academy of Sciences, USA 85:6968–6972
    [Google Scholar]
  15. Ogawa K., Shibata R., Kiyomasu T., Higuchi I., Kishida Y., Ishimoto A., Adachi A. 1989; Mutational analysis of the human immunodeficiency virus vpr open reading frame. Journal of Virology 63:4110–4114
    [Google Scholar]
  16. Popovic M., Sarngadharan M. G., Read E., Gallo R. C. 1984; Detection, isolation and continuous production of cytopathic retroviruses from patients with AIDS and pre-AIDS. Science 224:497–500
    [Google Scholar]
  17. Sakai H., Siomi H., Shida H., Shibata R., Kiyomasu T., Adachi A. 1990; Functional comparison of trans-activation by human retrovirus rev and rex genes. Journal of Virology 64:5833–5839
    [Google Scholar]
  18. Sakai H., Kawamura M., Sakuragi J., Sakuragi S., Shibata R., Ishimoto A., Ono N., Ueda S., Adachi A. 1993a; Integration is essential for efficient gene expression of human immunodeficiency virus type 1. Journal of Virology 67:1169–1174
    [Google Scholar]
  19. Sakai H., Shibata R., Sakuragi J., Sakuragi S., Kawamura M., Adachi A. 1993b; Cell-dependent requirement of human immunodeficiency virus type 1 Vif protein for maturation of virus particles. Journal of Virology 67:1663–1666
    [Google Scholar]
  20. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Schwartz S., Felber B. K., Fenyo E.-M., Pavlakis G. N. 1990; Env and vpu proteins of human immunodeficiency virus type 1 are produced from multiple bicistronic mRNAs. Journal of Virology 64:5448–5456
    [Google Scholar]
  22. Shibata R., Kawamura M., Sakai H., Hayami M., Ishimoto A., Adachi A. 1991; Generation of a chimeric human and simian immunodeficiency virus infectious to monkey peripheral blood mononuclear cells. Journal of Virology 65:3514–3520
    [Google Scholar]
  23. Strebel K., Klimkait T., Martin M. A. 1988; A novel gene of HIV-1, vpu, and its 16–kilodalton product. Science 241:1221–1223
    [Google Scholar]
  24. Strebel K., Klimkait T., Maldarelli F., Martin M. A. 1989; Molecular and biochemical analyses of human immunodeficiency virus type 1 vpu protein. Journal of Virology 63:3784–3791
    [Google Scholar]
  25. Takai T., Ohmori H. 1990; DNA transfection of mouse lymphoid cells by the combination of DEAE-dextran-mediated DNA uptake and osmotic shock procedure. Biochimica et Biophysica Acta 1048:105–109
    [Google Scholar]
  26. Terwilliger E. F., Cohen E. A., Lu Y., Sodroski J. G., Haseltine W. A. 1989a; Functional role of human immunodeficiency virus type 1 vpu . Proceedings of the National Academy of Sciences, USA 86:5163–5167
    [Google Scholar]
  27. Terwilliger E. F., Godin B., Sodroski J., Haseltine W. A. 1989b; Construction and use of a replication-competent human immunodeficiency virus (HIV-1) that expresses the chloramphenicol acetyltransferase enzyme. Proceedings of the National Academy of Sciences, USA 86:3857–3861
    [Google Scholar]
  28. Wigler M., Pelliser A., Silverstein S., Axel R., Urlaub G., Chasin L. 1979; DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells. Proceedings of the National Academy of Sciences, USA 76:1372–1376
    [Google Scholar]
  29. Willey R. L., Smith D. H., Laskey L. A., Theodore T. S., Earl P. L., Capon D. J., Martin M. A. 1988; In vitro mutagenesis identifies a region within the envelope gene of the human immunodeficiency virus that is critical for infectivity. Journal of Virology 62:139–147
    [Google Scholar]
  30. Willey R. L., Maldarelli F., Martin M., Strebel K. 1992; Human immunodeficiency virus type 1 vpu protein regulates the formation of intracellular gpl60-CD4 complexes. Journal of Virology 66:226–234
    [Google Scholar]
  31. Yao X. J., Gottlinger H., Haseltine W. A., Cohen E. A. 1992; Envelope glycoprotein and CD4 independence of v/iw-facilitated human immunodeficiency virus type 1 capsid export. Journal of Virology 66:5119–5126
    [Google Scholar]
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