1887

Abstract

The sequences of several cDNA clones of pear blister canker viroid (PBCVd) P1914T and P47A isolates have been determined. Seven out of eight P1914T clones analysed have a constant sequence which differs at six positions from that of the P2098T isolate reported previously. The remaining P1914T clone (8) has a single nucleotide substitution. The same six changes have been also observed in most of the ten P47A clones sequenced. However, some P47A clones show additional variability in positions on both strands of the central conserved region (CCR) and in another conserved sequence at the left-terminal region. This is the first report of a change affecting the upper strand of a viroid CCR. Reasons why such a change is tolerated are discussed. Infectivity bioassays have demonstrated that PBCVd is the causal agent of PBC disease.

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1995-10-01
2021-10-24
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References

  1. Diener T. O. 1986; Viroid processing: a model involving the central conserved region and hairpin. Proceedings of National Academy of Sciences, USA 83:58–62
    [Google Scholar]
  2. Flores R., Hernandez C., Llacer G., Desvignes J. C. 1991; Identification of a new viroid as the putative causal agent of pear blister canker disease. Journal of General Virology 72:1199–1204
    [Google Scholar]
  3. Gora A., Candresse T., Zagorski W. 1994; Analysis of the population structure of three phenotypically different PSTVd isolates. Archives of Virology 138:233–245
    [Google Scholar]
  4. Gross H. J., Domdey H., Lossow C. H., Jank P., Raba M., Alberty H., Sanger H. L. 1978; Nucleotide sequence and secondary structure of potato spindle tuber viroid. Nature 273:203–208
    [Google Scholar]
  5. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  6. Hashimoto J., Koganezawa H. 1987; Nucleotide sequence and secondary structure of apple scar skin viroid. Nucleic Acids Research 15:7045–7052
    [Google Scholar]
  7. Hernandez C., Elena S. F., Moya A., Flores R. 1992; Pear blister canker viroid is a member of the apple scar skin subgroup (apscaviroids) and also has sequence homology with viroids from other subgroups. Journal of General Virology 73:2502–2507
    [Google Scholar]
  8. Holland J., Spindler K., Horodyski F., Grabau E., Nichol S., Vandepol S. 1982; Rapid evolution of RNA genomes. Science 215:1577–1585
    [Google Scholar]
  9. Koltunow A. M., Rezaian M. A. 1988; Grapevine yellow speckle viroid: structural features of a new viroid group. Nucleic Acids Research 16:849–864
    [Google Scholar]
  10. Koltunow A. M., Rezaian M. A. 1989; A scheme for viroid classification. Intervirology 30:194–201
    [Google Scholar]
  11. Owens R. A., Khurana S. M. P., Smith D. R., Singh M. N., Garg I. D. 1992; A new mild strain of potato spindle tuber viroid isolated from wild Solanum spp. in India. Plant Disease 76:527–529
    [Google Scholar]
  12. Puchta H., Herold T., Verhoeven K., Roenhorst A., Ramm K., Schmidt-Puchta W., Sanger H. L. 1990a; A new strain of potato spindle tuber viroid (PSTVd-N) exhibits major sequence differences as compared to all other PSTVd strains sequenced so far. Plant Molecular Biology 15:509–511
    [Google Scholar]
  13. Puchta H., Luckinger R., Yang X., Hadidi A., Sanger H. L. 1990b; Nucleotide sequence and secondary structure of apple scar skin viroid (ASSVd) from China. Plant Molecular Biology 14:1065–1067
    [Google Scholar]
  14. Schnolzer M., Haas B., Ramm K., Hofmann H., Sanger H. L. 1985; Correlation between structure and pathogenicity of potato spindle tuber viroid (PSTVd). EMBO Journal 4:2181–2190
    [Google Scholar]
  15. Spieker R. L., Haas B., Charng Y. C., Freimuller K., Sanger H. L. 1990; Primary and secondary structure of a new viroid ‘species’ (CbVd 1). present in the Coleus blumei cultivar ‘Bienvenue’. Nucleic Acids Research 18:3998
    [Google Scholar]
  16. Visvader J. E., Symons R. H. 1985; Eleven new sequence variants of citrus exocortis viroid and the correlation of sequence with pathogenicity. Nucleic Acids Research 13:2907–2920
    [Google Scholar]
  17. Visvader J. E., Forster A. C., Symons R. H. 1985; Infectivity and in vitro mutagenesis of monomeric cDNA clones of citrus exocortis viroid indicate the site of processing of viroid precursors. Nucleic Acids Research 13:5843–5856
    [Google Scholar]
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