RT Journal Article SR Electronic(1) A1 Clark, W. Gregg A1 Fitchen, John A1 Nejidat, Ali A1 Deom, C. Michael A1 Beachy, Roger N.YR 1995 T1 Studies of coat protein-mediated resistance to tobacco mosaic virus (TMV). II. Challenge by a mutant with altered virion surface does not overcome resistance conferred by TMV coat protein JF Journal of General Virology, VO 76 IS 10 SP 2613 OP 2617 DO https://doi.org/10.1099/0022-1317-76-10-2613 PB Microbiology Society, SN 1465-2099, AB Transgenic tobacco plants expressing the coat protein (CP) gene of the U1 strain of tobacco mosaic virus (TMV) exhibit CP-mediated resistance (CP-MR) against some, but not all, tobamoviruses. To investigate the role of the amino acid sequences on the surface of the challenge virus in CP-MR, mutant strains of U1 TMV were constructed to contain the amino or carboxy termini of the CP of Sunn hemp mosaic tobamovirus (SHMV). The modified virus was unable to overcome CP-MR in transgenic plants that contained the TMV CP. In contrast, TMV in which the CP was replaced by the SHMV CP overcame CP-MR to the same extent as did SHMV. We conclude that CP-MR conferred by TMV CP involves interactions between amino acid sequences of the challenge viruses and the transgene protein other than those on the surface of the challenge virus., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-76-10-2613