1887

Abstract

The gene for the major capsid protein (hexon) of fowl adenovirus serotype 10 (FAV-10) has been identified by the use of the expression vector pGEX and rabbit polyclonal antisera raised against FAV-10. The nucleotide sequence of the entire hexon gene has been determined. Sequence analysis revealed an open reading frame of 2808 bp coding for a putative polypeptide 936 amino acids long with a molecular mass of 105.5 kDa. The translation initiation codon has a local sequence which conforms with the optimal translation start sequence of CC(A/G)CCATGG. The location of the hexon gene in the FAV genome was from 46.85 to 52.81 map units, which is to the left of the hexon gene in the genomes of both bovine and human adenoviruses (52.4 to 60.5 map units). A splice acceptor site was identified 12 bp upstream of the initiation codon by using mRNA and PCR. It had the sequence TAGG which conforms to the consensus sequence of (C/T)AGG. Comparison of the amino acid sequence of the FAV-10 hexon with those of the bovine, human and murine hexon gene products revealed highest levels of identity occurring in the regions corresponding to the pedestals which form the base region of the hexon, and the lowest levels of identity in the regions corresponding to the loops which are exposed to the external environment.

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1995-10-01
2024-12-06
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References

  1. Akusjarvi G., Pettersson U. 1978; Nucleotide sequence at the junction between the coding region of the adenovirus 2 hexon messenger RNA and its leader sequence. Proceedings of the National Academy of Sciences, USA 75:5822–5826
    [Google Scholar]
  2. Alestrom P., Stenlund A., Li P., Bellett A., Pettersson U. 1982a; Sequence homology between avian and human adenoviruses. Journal of Virology 42:306–310
    [Google Scholar]
  3. Alestrom P., Stenlund A., Li P., Pettersson U. 1982b; A common sequence in the inverted terminal repetitions of human and avian adenoviruses. Gene 18:193–197
    [Google Scholar]
  4. Bellett A. J. D., Younghusband H. B. 1972; Replication of the DNA of chick embryo lethal orphan virus. Journal of Molecular Biology 72:691–709
    [Google Scholar]
  5. Berget S. M., Moore C., Sharp P. A. 1977; Spliced segments at the 5′ terminus of adenovirus 2 late mRNA. Proceedings of the National Academy of Sciences, USA 74:3171–3175
    [Google Scholar]
  6. Cai F., Weber J. M. 1993; Organization of the avian adenovirus genome and the structure of its endopeptidase. Virology 196:358–362
    [Google Scholar]
  7. Chow L. T., Gelinas R. E., Broker T. R., Roberts R. J. 1977; An amazing sequence arrangement at the 5′ ends of adenovirus 2 messenger RNA. Cell 12:18
    [Google Scholar]
  8. Fenner F., Bachmann P. A., Gibbs E. P. J., Murphy F. A., Studdert M. J., White D. O. 1987; Adenoviridae. In Veterinary Virology pp 329–337 Orlando, Florida: Academic Press;
    [Google Scholar]
  9. Ginsberg H. S., Pereira H. G., Valentine R. C., Wilcox W. C. 1966; A proposed terminology for the adenovirus antigens and virion morphological subunits. Virology 28:782–783
    [Google Scholar]
  10. Hu S.-L., Hays W. W., Potts D. E. 1984; Sequence homology between bovine and human adenoviruses. Journal of Virology 49:604–608
    [Google Scholar]
  11. Jones N. C. 1986; Genetic elements governing adenoviral gene expression. In Adenovirus DNA: The Viral Genome and its Expression pp 161–191 Edited by Doerfler W. Boston: Martinus Nijhoff;
    [Google Scholar]
  12. Kinloch R., MacKay N., Mantner V. 1984; Adenovirus hexon: sequence comparison of subgroup C serotypes 2 and 5. Journal of Biological Chemistry 259:6431–6436
    [Google Scholar]
  13. Kozak M. 1984; Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Research 12:857–872
    [Google Scholar]
  14. Kozak M. 1986; Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44:2831–292
    [Google Scholar]
  15. Kozak M. 1987; At least six nucleotides preceding the AUG initiator codon enhance translation in mammalian cells. Journal of Molecular Biology 196:947–950
    [Google Scholar]
  16. Li P., Bellett A. J. D., Parish C. R. 1983; A comparison of the terminal protein and hexon polypeptides of avian and human adenoviruses. Journal of General Virology 64:1375–1379
    [Google Scholar]
  17. Li P., Bellett A. J. D., Parish C. R. 1984; DNA-binding proteins of chick embryo lethal orphan virus: lack of complementation between early proteins of avian and human adenoviruses. Journal of General Virology 65:1817–1825
    [Google Scholar]
  18. McFerran J. B., Connor T. J. 1977; Further studies on the classification of fowl adenoviruses. Avian Diseases 21:585–595
    [Google Scholar]
  19. Mount S. M. 1982; A catalogue of splice junction sequences. Nucleic Acids Research 10:459–172
    [Google Scholar]
  20. Norrby E. 1969; The relationship between the soluble antigens and the virion of adenovirus type 3. IV. Immunological complexity of soluble components. Virology 37:565–576
    [Google Scholar]
  21. Norrby E., Wadell G. 1969; Immunological relationships between hexons of certain human adenoviruses. Journal of Virology 4:663–670
    [Google Scholar]
  22. Norrby E., Bartha A., Boulanger P., Dreizin R. S., Ginsberg H. S., Kalter S. S., Kawamura H., Rowe W. P., Russell W. C., Schlesinger R. W., Wigand R. 1976; Adenoviridac. Intervirology 7:117–125
    [Google Scholar]
  23. Philipson L. 1979; Adenovirus proteins and their messenger RNAs. Advances in Virus Research 25:357–105
    [Google Scholar]
  24. Roberts M. M., White J. L., Grutter M. G., Burnett R. M. 1986a; Three dimensional structure of the adenoviurs major coat protein hexon. Science 232:1148–1151
    [Google Scholar]
  25. Roberts R. J., Akusjarvi G., Alestrom P., Gelinas R. E., Gingeras T. R., Sciaky D., Pettersson U. 1986b; A consensus sequence for the adenovirus-2 genome. In Adenovirus DNA: The Viral Genome and its Expression pp 151 Edited by Doerfler W. Boston: Martinus Nijhoff;
    [Google Scholar]
  26. Robinson A. J., Younghusband H. B., Bellett A. J. D. 1973; A circular DNA-protein complex from adenoviruses. Virology 56:54–69
    [Google Scholar]
  27. Sheppard M., Erny K. M. 1989; DNA sequence analysis of the inverted terminal repeats of a non-oncogenic avian adenovirus. Nucleic Acids Research 17:39–95
    [Google Scholar]
  28. Sheppard M., Trist H. 1992; Characterization of the avian adenovirus penton base. Virology 188:881–886
    [Google Scholar]
  29. Sheppard M., Trist H. 1993; The identification of genes for the major core proteins of fowl adenovirus serotype 10. Archives of Virology 132:443–149
    [Google Scholar]
  30. Toogood C. I. A., Hay R. T. 1988; DNA sequence of the adenovirus type 41 hexon gene and predicted structure of the protein. Journal of General Virology 69:2291–2301
    [Google Scholar]
  31. Toogood C. I. A., Murali R., Burnett R. M., Hay R. T. 1989; The adenovirus type 40 hexon: sequence, predicted structure and relationship to other adenovirus hexons. Journal of General Virology 70:3203–3214
    [Google Scholar]
  32. Weber J. M., Cai F., Murali R., Burnett R. M. 1994; Sequence and structural analysis of murine adenovirus type 1 hexon. Journal of General Virology 75:141–147
    [Google Scholar]
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