1887

Abstract

We investigated the relationships between amino acid polymorphisms of the prion protein (PrP), restriction fragment length polymorphisms (RFLP) of the PrP gene and the incidence of natural scrapie in Japan. Six variant alleles of the PrP gene were found in healthy sheep. Based on the substitutions at codons 112, 136, 154 and 171, these allelic variants were designated PrP, PrP, PrP, PrP, PrP and PrP. Each RFLP haplotype (e1h1, e1h2 or e3h1) consisted of multiple alleles including PrP. Three of these variant alleles were found in scrapie-affected Suffolk sheep. PrP was associated with high disease incidence, PrP and PrP were associated with low disease incidence. We found that one scrapie-affected Suffolk was homozygous for PrP and four PrP-positive Suffolks carried PrP. Both of two scrapie-affected Corriedales and two out of three scrapie-affected cross-breeds between Suffolk and Corriedale carried PrP, suggesting that this allele associates with high incidence of scrapie in Corriedale and its cross-breeds.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-10-2577
1995-10-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/10/JV0760102577.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-10-2577&mimeType=html&fmt=ahah

References

  1. Belt P. B. G. M., Muileman I. H., Schreuder B. E. C., Bos-de Ruijter J., Gielkens A. L. J., Smits M. A. 1995; Identification of five allelic variants of the sheep PrP gene and their association with natural scrapie. Journal of General Virology 76:509–517
    [Google Scholar]
  2. Foster J. D., Dickinson A. G. 1988; Genetic control of scrapie in Cheviot and Suffolk sheep. Veterinary Record123–159
    [Google Scholar]
  3. Goldmann W., Hunter N., Foster J. D., Salbaum J. M., Beyreuther K., Hope J. 1990; Two alleles of a neural protein gene linked to scrapie in sheep. Proceedings of the National Academy of Sciences, USA 87:2476–2480
    [Google Scholar]
  4. Goldmann W., Hunter N., Benson G., Foster J. D., Hope J. 1991; Different scrapie-associated fibril proteins (PrP) are encoded by lines of sheep selected for different alleles of the Sip gene. Journal of General Virology 72:2411–2417
    [Google Scholar]
  5. Goldmann W., Hunter N., Smith G., Foster J. D., Hope J. 1994; PrP genotype and agent effects in scrapie: change in allelic interaction with different isolates of agent in sheep, a natural host of scrapie. Journal of General Virology 75:989–995
    [Google Scholar]
  6. Higuchi R. G., Ochman H. 1989; Production of single-stranded DNA templates by exonuclease digestion following the polymerase chain reaction. Nucleic Acids Research 17:5865
    [Google Scholar]
  7. Hunter N. 1993; Genetic control of scrapie incidence in sheep and its relevance for bovine spongiform encephalopathy in cattle. Medical Virology 3:195–200
    [Google Scholar]
  8. Hunter N., Goldmann W., Benson G., Foster J. D., Hope J. 1993; Swaledale sheep affected by natural scrapie differ significantly in PrP genotype frequencies from healthy sheep and those selected for reduced incidence of scrapie. Journal of General Virology 74:1025–1031
    [Google Scholar]
  9. Hunter N., Goldmann W., Smith G., Hope J. 1994; The association of a codon 136 PrP gene variant with the occurrence of natural scrapie. Archives of Virology 137:171–177
    [Google Scholar]
  10. Ikegami Y., Ito M., Isomura H., Momotani E., Sasaki K., Muramatsu Y., Ishiguro N., Shinagawa M. 1991; Preclinical and clinical diagnosis of scrapie by detection of PrP protein in tissues of sheep. Veterinary Record 128:271–275
    [Google Scholar]
  11. Laplanche J. L., Chatelain J., Westaway D., Thomas S., Dussaucy M., Brugere-Picoux J., Launay J. M. 1993; PrP polymorphisms associated with natural scrapie discovered by denaturing gradient gel electrophoresis. Genomics 15:30–37
    [Google Scholar]
  12. Maciulis A., Hunter N., Wang S., Goldmann W., Hope J., Foote W. C. 1992; Polymorphisms of a scrapie-associated fibril protein (PrP) gene and their association with susceptibility to experimentally induced scrapie in Cheviot sheep in the United States. American Journal of Veterinary Research 53:1957–1960
    [Google Scholar]
  13. Muramatsu Y., Tanaka K., Horiuchi M., Ishiguro N., Shinagawa M., Matsui T., Onodera T. 1992; A specific RFLP type associated with the occurrence of sheep scrapie in Japan. Archives of Virology 127:1–9
    [Google Scholar]
  14. Muramatsu Y., Onodera A., Horiuchi M., Ishiguro N., Shinagawa M. 1993; Detection of PrPSc in sheep at the preclinical stage of scrapie and its significance for diagnosis of insidious infection. Archives of Virology 134:427–432
    [Google Scholar]
  15. Onodera A., Ikeda T., Horiuchi M., Ishiguro N., Onuma M., Hirano N., Mikami T., Honda E., Hirai K., Kai K., Yugi H., Muramatsu Y., Shinagawa M. 1994; Survey of natural scrapie in Japan: analysis of RFLP types of the PrP gene and detection of PrPSc mainly in Suffolk sheep. Journal of Veterinary Medical Science 56:627–632
    [Google Scholar]
  16. Saiki R. K., Scharf S., Faloona F., Mullis K. B., Horn G. T., Erlich H. A., Arnheim N. 1985; Enzymatic amplification of β- globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230:1350–1354
    [Google Scholar]
  17. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  18. Westaway D., Zuliani V., Cooper C. M., Costa M. D., Neuman S., Jenny A. L., Detwiler L., Prusiner S. B. 1994; Homozygosity for prion protein alleles encoding glutamine-171 renders sheep susceptible to natural scrapie. Genes & Development 8:959–969
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-10-2577
Loading
/content/journal/jgv/10.1099/0022-1317-76-10-2577
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error