1887

Abstract

The complete nucleotide sequence of the genome of the fish rhabdovirus infectious haematopoietic necrosis virus (IHNV) has been determined after cDNA cloning of the viral genomic RNA. Sequence analysis showed the presence of six open reading frames encoding the nucleoprotein N, the matrix proteins M1 and M2, the glycoprotein G, a so-called non-structural protein NV, and the RNA polymerase L. The genome organization is 3′ N-M1-M2-G-NV-L 5′. The extreme 5′ and 3′ ends of the genome were sequenced after RNA ligation or RACE. Prokaryotic expression products of the open reading frames predicted to encode the matrix proteins M1 and M2, the glycoprotein G and the NV protein reacted with rabbit anti-IHNV serum thereby confirming their identity. This is the first complete nucleotide sequence of a fish rhabdovirus. Knowledge of the complete sequence is an essential prerequisite for future manipulation of the genome and also serves to provide gene- and protein-specific reagents for use in further examination of the replication of the fish rhabdoviruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-10-2519
1995-10-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/10/JV0760102519.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-10-2519&mimeType=html&fmt=ahah

References

  1. Baer G. M., Bellini W. J., Fishbein D. B. 1990; Rhabdoviruses. In Virology 2nd edn, pp 883–930 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  2. Benmansour A., Paubert G., Bernard J., de Kinkelin P. 1994; The polymerase-associated protein (Ml) and the matrix protein (M2) from a virulent and an avirulent strain of viral hemorrhagic septicemia virus (VHSV), a fish rhabdovirus. Virology 198:602–612
    [Google Scholar]
  3. Bernard J., Lecocq-Xhonneux F., Rossius M., Thiry M. E., de Kinkelin P. 1990; Cloning and sequencing the messenger RNA of the N gene of viral haemorrhagic septicaemia virus. Journal of General Virology 71:1669–1674
    [Google Scholar]
  4. Bovo G., Giorgetti G., Jorgensen P. E. V., Olesen N. J. 1987; Infectious haematopoietic necrosis: first detection in Italy. Bulletin of the European Association of Fish Pathology 7:124
    [Google Scholar]
  5. Caverner D. C. 1987; Comparison of consensus sequence flanking translational start sites in Drosophila and vertebrates. Nucleic Acids Research 15:1353–1361
    [Google Scholar]
  6. Chomzynski P., Sacchi N. 1987; Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  7. Conzelmann K.-K., Cox J. H., Schneider L. G., Thiel H.-J. 1990; Molecular cloning and complete nucleotide sequence of the attenuated rabies virus SAD B19. Virology 175:485–499
    [Google Scholar]
  8. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  9. Gallione C. J., Rose J. K. 1983; Nucleotide sequence of a cDNA clone coding for the entire glycoprotein from the New Jersey serotype of vesicular stomatitis virus. Journal of Virology 46:162–169
    [Google Scholar]
  10. Giesecke H., Neubert N., Obermaier B., Domdey H. 1992; Rapid sequencing of the Sendai virus 6.8 kb large (L) gene through primer walking with an automatic sequencer. Journal of Virological Methods 38:47–60
    [Google Scholar]
  11. Gilmore R. D., Leong J. C. 1988; The nucleocapsid gene of infectious hematopoietic necrosis virus, a fish rhabdovirus. Virology 167:644–648
    [Google Scholar]
  12. Higuchi Y., Miyahara Y., Kawano M., Tsurudome M., Matsumura H., Kusagawa S., Komada H., Nishio M., Ito Y. 1992; Sequence analysis of the large (L) protein of simian virus 5. Journal of General Virology 73:1005–1010
    [Google Scholar]
  13. Hill B. J., Underwood B. O., Smale C. J., Brown F. 1975; Physico–chemical and serological characterization of five rhabdo-viruses infecting fish. Journal of General Virology 27:369–378
    [Google Scholar]
  14. Kiuchi A., Roy P. 1984; Comparison of the primary sequence of spring viremia of carp virus M protein with that of vesicular stomatitis virus. Virology 134:238–243
    [Google Scholar]
  15. Koener J. F., Passavant C. W., Kurath G., Leong J. C. 1987; Nucleotide sequence of a cDNA clone carrying the glycoprotein gene of infectious hematopoietic necrosis virus, a fish rhabdovirus. Journal of Virology 61:1342–1349
    [Google Scholar]
  16. Kozak M. 1987; An analysis of the 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Research 15:8125–8148
    [Google Scholar]
  17. Kurath G., Leong J. C. 1985; Characterization of infectious hematopoietic necrosis virus mRNA species demonstrates a nonvirion rhabdovirus protein. Journal of Virology 53:462–168
    [Google Scholar]
  18. Kurath G., Leong J. C. 1987; Transcription in vitro of infectious haematopoietic necrosis virus, a fish rhabdovirus. Journal of General Virology 68:1767–1771
    [Google Scholar]
  19. Kurath G., Ahern K. G., Pearson G. D., Leong J. C. 1985; Molecular cloning of the six mRNA species of infectious hematopoietic necrosis virus, a fish rhabdovirus, and gene order determination by R-loop mapping. Journal of Virology 53:469–476
    [Google Scholar]
  20. Laurencin B. 1987; IHN in France. Bulletin of the European Association of Fish Pathologists 7:104
    [Google Scholar]
  21. McAllister P. E. 1979; Fish viruses and viral infections. In Comprehensive Virology vol 14 pp 401–470 Edited by Fraenkel-Conrat H., Wagner R. R. New York & London: Plenum Press;
    [Google Scholar]
  22. McIntosh K., Chanock R. M. 1990; Respiratory syncytial virus. In Virology 2nd edn, pp 1045–1072 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  23. Morimoto K., Ohkubo A., Kawai A. 1989; Structure and transcription of the glycoprotein gene of attenuated HEP-Flury strain of rabies virus. Virology 173:465–477
    [Google Scholar]
  24. Mühlberger E., Sanchez A., Randolf A., Will C., Kiley M. P., Klenk H. D., Feldmann H. 1992; The nucleotide sequence of the L gene of Marburg virus, a filovirus: homologies with paramyxoviruses and rhabdoviruses. Virology 187:534–547
    [Google Scholar]
  25. Murphy F. A., Kiley M. P., Fisher-Hoch S. P. 1990; Filoviridae. In Virology 2nd edn, pp 933–942 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  26. Ponce M. R., Micol J. L. 1992; PCR amplification of long DNA fragments. Nucleic Acids Research 20:623
    [Google Scholar]
  27. Rose J. K. 1980; Complete intergenic and flanking gene sequences from the genome of vesicular stomatitis virus. Cell 19:415–421
    [Google Scholar]
  28. Sacramento D., Badrane H., Bourhy H., Tordo N. 1992; Molecular epidemiology of rabies in France: comparison with vaccine strains. Journal of General Virology 73:1149–1158
    [Google Scholar]
  29. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  30. Sano T., Nishimura T., Okamoto N., Yamazaki T., Hanada H., Watanabe Y. 1977; Studies on viral diseases of Japanese fishes. VI. Infectious hematopoietic necrosis (IHN) of salmonids in the mainland of Japan. Journal of the Tokyo University of Fisheries 63:81–85
    [Google Scholar]
  31. Schnell M. J., Mebatsion T., Conzelmann K.-K. 1994; Infectious rabies viruses from cloned cDNA. EMBO Journal 13:4195–4203
    [Google Scholar]
  32. Schubert M., Harmison G. G., Meier E. 1984; Primary structure of the vesicular stomatitis virus polymerase (L) gene: evidence for a high frequency of mutations. Journal of Virology 51:505–514
    [Google Scholar]
  33. Shioda T., Iwasaki K., Shibuta H. 1986; Determination of the complete nucleotide sequence of the Sendai virus genome RNA and the predicted amino acid sequences of F, HN and L proteins. Nucleic Acids Research 14:1545–1563
    [Google Scholar]
  34. Stec D. S., Hill M. G., Collins P. L. 1991; Sequence analysis of the polymerase L gene of human respiratory syncytial virus and predicted phylogeny of nonsegmented negative-strand viruses. Virology 183:273–287
    [Google Scholar]
  35. Studier F. W., Moffatt B. A. 1986; Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. Journal of Molecular Biology 189:113–120
    [Google Scholar]
  36. Thiry M., Lecocq-Xhonneux F., Dheur I., Renard A., DE Kinkelin P. 1990; Molecular cloning of the mRNA coding for the G protein of the viral haemorrhagic septicaemia (VHS) of salmonids. Veterinary Microbiology 23:221–226
    [Google Scholar]
  37. Thiry M., Lecocq-Xhonneux F., Dheur I., Renard A., de Kinkelin P. 1991; Sequence of a cDNA carrying the glycoprotein gene and part of the matrix protein M2 gene of viral haemorrhagic septicaemia virus, a fish rhabdovirus. Biochimica et Biophysica Acta 1090:345–347
    [Google Scholar]
  38. Tordo N., Poch O., Ermine A., Kjeith G., Rougeon F. 1986; Walking along the rabies genome: is the large G-L intergenic region a remnant gene?. Proceedings of the National Academy of Sciences, USA 83:3914–3918
    [Google Scholar]
  39. Tordo N., Poch O., Ermine A., Kjeith G., Rougeon F. 1988; Completion of the rabies virus genome sequence determination: highly conserved domains among the L (polymerase) proteins of unsegmented negative-strand RNA viruses. Virology 165:565–576
    [Google Scholar]
  40. Tordo N., Dehaan P., Goldbach R., Poch O. 1992; Evolution of negative-stranded RNA genomes. Seminars in Virology 3:341–357
    [Google Scholar]
  41. Wagner R. R. 1990; Rhabdoviridae and their replication. In Virology 2nd edn, pp 867–881 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  42. Walker P. J., Byrne K. A., Riding G. A., Cowley J. A., Wang Y., McWilliam S. 1992; The genome of bovine ephemeral fever rhabdovirus contains two related glycoprotein genes. Virology 191:49–61
    [Google Scholar]
  43. Xu L., Mourich D. V., Engelking H. M., Ristow S., Arnzen J., Leong J. C. 1991; Epitope mapping and characterization of infectious hematopoietic necrosis virus glycoprotein, using fusion proteins synthesized in Escherichia coli . Journal of Virology 65:1611–1615
    [Google Scholar]
  44. Yang F., Lazzarini R. A. 1983; Analysis of the recombinant event generating a vesicular stomatitis virus deletion defective interfering particle. Journal of Virology 45:766–772
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-10-2519
Loading
/content/journal/jgv/10.1099/0022-1317-76-10-2519
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error