1887

Abstract

The ecdysteroid UDP-glucosyltransferase () gene isolated from a plaque-purified isolate of multinucleocapsid nuclear polyhedrosis virus (CfMNPV) was compared to its homologue from a defective MNPV virus (CfDEF) present in wild-type virus populations infecting the eastern spruce budworm, . The genes were located in the same relative position within the virus genomes and their genomic location and arrangement were similar to that found in MNPV (AcMNPV) and MNPV (OpMNPV). The genes encoded 491 and 494 amino acid open reading frames respectively, and were 67% identical at the amino acid level and 74% identical at the nucleotide level. Transcripts of the of CfMNPV peaked around 12 h post-infection (p.i.) and disappeared after 36 h p.i. Transcripts of the of CfDEF peaked between 6 and 9 h p.i. and were not detected 24 h p.i. The from CfMNPV was more similar to the partially sequenced identified from OpMNPV, at the nucleotide and amino acid levels, than it was to the from the CfDEF, AcMNPV, NPV, MNPV or MNPV. Phylogenetic analysis of supported the baculovirus evolution scheme suggested by polyhedrin sequence analysis.

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1995-10-01
2021-10-21
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References

  1. Anonymous 1994 Compendium of Canadian Forestry Statistics 1993 Ottawa: Canadian Council of Forest Ministers.;
    [Google Scholar]
  2. Arif B. M., Kuzio J., Faulkner P., Doerfler W. 1984; The genome of Choristoneura fumiferana nuclear polyhedrosis virus: molecular cloning and mapping of the EcoRI, BamHI, SmaI, XbaI and BglII restriction sites. Virus Research 1:605–614
    [Google Scholar]
  3. Arif B. M., Jamieson P. B., Sohi S. S., Kaupp W., MacDonald J. A. 1994; Symbiotic-like relationship between two MNPVs of the spruce budworm, Choristoneura fumiferana. . In Proceedings of the VIth International Colloquium on Invertebrate Pathology and Microbial Control p 145 XXVIIth Annual Meeting of the Society for Invertebrate Pathology, Montpellier, France, 28 August–2 September 1994
    [Google Scholar]
  4. Blissard G., Kogan P. H., Wei R., Rohrmann G. 1992; A synthetic early promoter from a baculovirus: roles of the TATA box and conserved start site CAGT sequence in basal levels of transcription. Virology 190:783–793
    [Google Scholar]
  5. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  6. Croizier G., Croizier L., Argaud O., Poudevigne D. 1994; Extension of Autographa californica nuclear polyhedrosis virus host range by interspecific replacement of a short DNA sequence in the pl43 helicase gene. Proceedings of the National Academy of Sciences, USA 91:48–52
    [Google Scholar]
  7. Cunningham J. C., Van Frankenhuyzen K. 1991; Microbial insecticides in forestry. Forestry Chronicle 67:473–180
    [Google Scholar]
  8. Higgins D. G., Sharp P. M. 1988; CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene 73:237–244
    [Google Scholar]
  9. Nissen M. S., Friessen P. D. 1989; Molecular analysis of the transcriptional regulatory region of an early baculovirus gene. Journal of Virology 63:493–503
    [Google Scholar]
  10. O’Reilly D. R., Miller L. K. 1989; A baculovirus blocks insect moulting by producing ecdysteroid UDP-glucosyl transferase. Science 245:1110–1112
    [Google Scholar]
  11. O’Reilly D. R., Miller L. K. 1990; Regulation of expression of a baculovirus ecdysteroid UDP-glucosyltransferase gene. Journal of Virology 64:1321–1328
    [Google Scholar]
  12. O’Reilly D. R., Miller L. K. 1991; Improvement of a baculovirus pesticide by deletion of the egt gene. Bio/Technology 9:1086–1089
    [Google Scholar]
  13. Passarelli A. L., Miller L. K. 1993; Identification and characterization of lef- 1, a baculovirus gene involved in late and very late gene expression. Journal of General Virology 67:3481–3488
    [Google Scholar]
  14. Pearson M. N., Bjornson R. M., Ahrens C., Rohrmann G. F. 1993; Identification and characterization of a putative origin of DNA replication in the genome of a baculovirus pathogenic for Orgyia pseudotsugata . Virology 197:715–725
    [Google Scholar]
  15. Riegel C. I., Lanner-Herrera C., Slavicek J. M. 1994a; Identification and characterization of the ecdysteroid UDP-glucosyltransferase gene of the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus. Journal of General Virology 75:829–838
    [Google Scholar]
  16. Riegel C. I., Park E. J., Burand J., Slavicek J. M. 1994b; Generation of an improved biopesticide by deletion of the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus ecdysteroid UDP-glucosyltransferase gene. . In Proceedings of the VIth International Colloquium on Invertebrate Pathology and Microbial Control p 424. XXVIIth Annual Meeting of the Society for Invertebrate Pathology, Montpellier, France, 28 August-2 September 1994
    [Google Scholar]
  17. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual. New York:: Cold Spring Harbour Laboratory.;
    [Google Scholar]
  18. Swafford D. L. 1990 PAUP: Phylogenetic Analysis Using Parsimony, Version 3.0. Champaign: Illinois Natural History Survey;
    [Google Scholar]
  19. Wood H. A., Granados R. R. 1991; Genetically engineered baculoviruses as agents for pest control. Annual Review of Microbiology 45:69–87
    [Google Scholar]
  20. Zanotto P. M. A., Kessing B. D., Maruniak J. E. 1993; Phylogenetic interrelationships among baculoviruses: evolutionary rates and host associations. Journal of Invertebrate Pathology 62:147–164
    [Google Scholar]
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