1887

Abstract

SUMMARY

In the search for factors that influence the outcome of human polyomavirus JC (JCV) infection, the roles not only of host-related immunological control but also of virus-dependent regulatory steps have to be taken into account. Besides cell-specific control of early expression of the multifunctional virus protein large tumour antigen (T Ag), control mechanisms involve individual steps of the DNA replication process. For the analysis of T Ag DNA binding, the protein was expressed by an adenovirus hybrid vector in the 293 cell line to provide saturating amounts of JCV T Ag. After determination of the size and immunoreactivity, functional activity was analysed by specific DNA binding. To avoid the interference of cellular proteins, T Ag was immunoprecipitated prior to the reaction. Binding to T Ag-binding sites I and II within a 141 bp DNA segment in the control region was analysed using deletion mutants of a JCV subtype from brain tissue of a patient with fatal central nervous system disease. The specificity of the binding was confirmed by recombinant T Ag binding to origin of DNA replication (ori) sequences of wild-type JCV genomes. These data document that recombinant T Ag overexpressed by the adenovirus vector in eukaryotic cells was JCV-specific, had the expected length and exhibited specific ori-binding activity, thus providing the essential tool for future analysis of virus-host interactions at the level of viral DNA replication.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-1-83
1995-01-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/1/JV0760010083.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-1-83&mimeType=html&fmt=ahah

References

  1. Amirhaeri S., Wohlrab F., Major E. O., Wells R. 1988; Unusual DNA structure in the regulatory region of the human papovirus JC virus. Journal of Virology 62:922–931
    [Google Scholar]
  2. Arthur R. R., Shah K. V. 1989; Occurrence and significance of papovaviruses BK and JC in the urine. Progress in Medical Virology 36:42–61
    [Google Scholar]
  3. Berkner K. L. 1992; Expression of heterologous sequences in adenoviral vectors. Current Topics in Microbiology and Immunology 158:39–66
    [Google Scholar]
  4. Borowiec J. A., Dean F. B., Hurwitz J. 1991; Differential induction of structural changes in the simian virus 40 origin of replication by T antigen. Journal of Virology 65:1228–1235
    [Google Scholar]
  5. Deb S. P., Deb S. 1989; Preferential binding of simian virus 40 T antigen dimers to origin region I. Journal of Virology 63:2901–2907
    [Google Scholar]
  6. DePamphilis M. L. 1993; Eukaryotic DNA replication: anatomy of an origin. Annual Review of Biochemistry 62:29–63
    [Google Scholar]
  7. Dörries K. 1984; Progressive multifocal leukoencephalopathy: analysis of JC virus DNA from brain and kidney tissue. Virus Research 1:25–38
    [Google Scholar]
  8. Fanning E., Knippers R. 1992; Structure and function of simian virus 40 large tumor antigen. Annual Review of Biochemistry 61:55–85
    [Google Scholar]
  9. Feigenbaum L., Khalili K., Major E., Khoury G. 1987; Regulation of the host range of human papovavirus JCV. Proceedings of the National Academy of SciencesU.S.A. 84:3695–3698
    [Google Scholar]
  10. Fiers W., Contreras R., FIaegeman G., Rogiers R., Van de Voorde A., Van Heuverswyn H., Van Herreweghe J., Volckaert G., Ysebaert M. 1978; Complete nucleotide sequence of SV40 DNA. Science 293113–120
    [Google Scholar]
  11. Frisque R. J., White F. A. III 1992; The molecular biology of JC virus, causative agent of progressive multifocal leukoencephalopathy. In Molecular Neurovirology25–158 Roos R. P. Clifton, New Jersey: The Humana Press;
    [Google Scholar]
  12. Frisque R., Bream G. L., Cannella M. T. 1984; Human polyomavirus JC genome. Journal of Virology 51:458–469
    [Google Scholar]
  13. Graham F. L., van der Eb A. J. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–467
    [Google Scholar]
  14. Graham F. L., Smiley J., Russell W. C., Nairn R. 1977; Characteristics of a human cell line transformed by DNA from human adenovirus type 5. Journal of General Virology 36:59–72
    [Google Scholar]
  15. Haggerty S., Walker D. L., Frisque R. J. 1989; JC virus-simian virus 40 genomes containing heterologous regulatory signals and chimeric early regions: identification of regions restricting trans- formation by JC virus. Journal of Virology 63:2180–2190
    [Google Scholar]
  16. Harlow E., Lane D. 1988; . Antibodies: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Harlow E., Crawford L. V., Pim D. C., Williamson N. M. 1981; Monoclonal antibodies specific for simian virus 40 tumor antigens. Journal of Virology 39:861–869
    [Google Scholar]
  18. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
    [Google Scholar]
  19. Hinzpeter M., Fanning E., Deppert W. 1986; A new sensitive target-bound DNA binding assay for SV40 large T antigen. Virology 148:159–167
    [Google Scholar]
  20. Jones N., Shenk T. 1979; Isolation of an adenovirus type 5 host range deletion mutant defective for transformation of rat embryo cells. Cell 17:683–689
    [Google Scholar]
  21. Kerr D. A., Chang C. -F., Gordon J., Bjornsti M. -A., Khalili K. 1993; Inhibition of human neurotropic virus (JCV) DNA replication in glial cells by camptothecin. Virology 196:612–618
    [Google Scholar]
  22. Lamarche N., Massie B., Richer M., Paradis H., Langelier Y. 1990; High level expression in 293 cells of the herpes simplex virus type 2 ribonucleotide reductase subunit 2 using an adenovirus vector. Journal of General Virology 71:1785–1795
    [Google Scholar]
  23. Li J. J., Kelly T. J. 1985; Simian virus 40 DNA replication in vitro: specificity of initiation and evidence for bidirectional rep¬lication. Molecular and Cellular Biology 6:3086–3093
    [Google Scholar]
  24. Loeber G., Dörries K. 1988; DNA rearrangements in organ- specific variants of polyomavirus JC strain GS. Journal of Virology 62:1730–1735
    [Google Scholar]
  25. Lynch K. J., Frisque R. J. 1990; Identification of critical elements within the JC virus DNA replication origin. Journal of Virology 64:5812–5822
    [Google Scholar]
  26. Lynch K. J., Frisque R. J. 1991; Factors contributing to the restricted DNA replicating activity of JC virus. Virology 180:306–317
    [Google Scholar]
  27. McKay R. D. G. 1981; Binding of a simian virus 40 T antigen- related protein to DNA. Journal of Molecular Biology 145:471–188
    [Google Scholar]
  28. McVey D., Brizuela L., Mohr I., Marshak D. R., Gluzman Y., Beach D. 1989; Phosphorylation of large tumour antigen by cdc2 stimulates SV40 DNA replication. Nature 341:503–507
    [Google Scholar]
  29. Major E. O., Traub R. G. 1986; JC virus T protein during productive infection in human fetal brain and kidney cells. Virology 148:221–225
    [Google Scholar]
  30. Major E. O., Amemiya K., Tornatore C. S., Houff S. A., Berger J. R. 1992; Pathogenesis and molecular biology of progressive multifocal leukoencephalopathy, the JC virus-induced demyelinating disease of the human brain. Clinical Microbiology Reviews 5:49–73
    [Google Scholar]
  31. Mandl C. W., Frisque R. J. 1986; Characterization of cells transformed by the human polyomavirus JC virus. Journal of General Virology 67:1733–1739
    [Google Scholar]
  32. Martin J. D., King D. M., Slauch J. M., Frisque R. J. 1985; Differences in regulatory sequences of naturally occurring JC virus variants. Journal of Virology 53:306–311
    [Google Scholar]
  33. Myers C., Frisque R. J., Arthur R. R. 1989; Direct isolation and characterization of JC virus from urine samples of renal and bone marrow patients. Journal of Virology 63:4445–1449
    [Google Scholar]
  34. Parsons R., Anderson M. E., Tegtmeyer P. 1990; Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen. Journal of Virology 64:509–518
    [Google Scholar]
  35. Precious B., Russell W. C. 1985; Growth, purification and titration of adenovirus. In Virology, a Practical Approach193–205 Mahy B. W. J. Oxford: IRL Press;
    [Google Scholar]
  36. Prives C. 1990; The replication functions of SV40 T antigen are regulated by phosphorylation. Cell 61:735–738
    [Google Scholar]
  37. Rigby P. W. J., Lane D. P. 1983; Structure and function of simian virus 40 large T antigen. In Advances in Viral Oncology 331–57 Klein G. New York: Raven Press;
    [Google Scholar]
  38. Sambrook J., Fritsch E. F., Maniatis T. 1989; . Molecular Cloning: A Laboratory Manual, 2. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  39. Simmons D. T., Loeber G., Tegtmeyer P. 1990; Four major sequence elements of simian virus 40 large T antigen coordinate its specific and non-specific DNA binding. Journal of Virology 64:1973–1983
    [Google Scholar]
  40. Simmons D. T., Wun-Kim K., Young W. 1990; Identification of simian virus 40 T antigen residues important for specific and non- specific binding to DNA and for helicase activity. Journal of Virology 64:4858–1865
    [Google Scholar]
  41. Sock E., Wegner M., Grummt F. 1991; DNA replication of human polyomavirus JC is stimulated by NF-I in vivo. Virology 182:298–308
    [Google Scholar]
  42. Sock E., Wegner M., Fortunato E. A., Grummt F. 1993; Large T-antigen and sequences within the regulatory region of JC virus both contribute to the features of JC virus DNA replication. Virology 197:537–548
    [Google Scholar]
  43. Solnick D. 1981; Construction of an adenovirus-SV40 recombinant producing SV40 T antigen from an adenovirus late promoter. Cell 24:135–143
    [Google Scholar]
  44. Stetter G., Müller D., Montenarh M. 1988; SV40 T antigen binding to site II is functionally separated from binding to site I. Virology 164:309–317
    [Google Scholar]
  45. Stoner G. L., Ryschkewitsch C. F., Walker D. L., Soffer D., Webster H. F. 1988; A monoclonal antibody to SV40 large T antigen labels a nuclear antigen in JC virus-transformed cells and in progressive multifocal leucoencephalopathy (PML) brain infected with JC virus. Journal of Neuroimmunology 17:331–345
    [Google Scholar]
  46. Tabor S., Richardson C. C. 1987; DNA sequence analysis with a modified T7 DNA polymerase. Proceedings of the National Academy of SciencesU.S.A. 84:4767–4771
    [Google Scholar]
  47. Tavis J. E., Frisque R. J. 1991; Altered DNA binding and replication activities of JC virus T antigen mutants. Virology 183:239–250
    [Google Scholar]
  48. Walker D. L., Frisque R. J. 1986; The biology and molecular biology of JC virus. In The Papovaviridae: The Polyomaviruses327–377 Salzman N. P. New York: Plenum Press;
    [Google Scholar]
  49. Walker D. L., Padgett B. L. 1983; Progressive multifocal leukoencephalopathy. Comprehensive Virology 18:161–193
    [Google Scholar]
  50. Walker D. L., Padgett B. L., ZuRhein G. M., Albert A. D., Marsh R. F. 1973; Human polyomavirus (JC): induction of brain tumors in hamsters. Science 181:674–676
    [Google Scholar]
  51. Yogo Y., Kitamura T., Sugimoto C., Ueki T., Aso Y., Hara K., Taguchi F. C. 1990; Isolation of a possible archetypal JC virus DNA sequence from nonimmunocompromised individuals. Journal of Virology 64:3139–3143
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-1-83
Loading
/content/journal/jgv/10.1099/0022-1317-76-1-83
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error