1887

Abstract

SUMMARY

A porcine rotavirus strain, CN86, originally isolated from rotavirus-infected piglets in Argentina, has been shown to possess unique characteristics. It was the first animal strain described to be antigenically related to human serotype G1 and the standard counterpart of another porcine strain showing rearrangement of genome segment 11. Owing to these features, molecular characterization of this virus seemed relevant. The gene encoding the major inner capsid protein, VP6, was cloned and its nucleotide sequence was determined. Comparative analysis of the deduced amino acid sequence of CN86 VP6 with those representing the four different subgroups showed that it is more closely related to subgroup II human Wa and porcine Gottfried strains, albeit to a lesser extent than they are to each other. Despite exhibiting sequence divergence, CN86 VP6 has 12 out of the 14 residues expected to be conserved in strains bearing subgroup II specificity. Interestingly, CN86 VP6 shows a high degree of homology with VP6 of porcine strain YM rotavirus which, although being closely related to subgroup II strains, has been serologically characterized as subgroup I. Subgroup II reactivity of CN86 strain, predicted by sequence analysis, was confirmed by ELISA with subgroup-specific monoclonal antibodies. Taken together, our results provide evidence for the existence of a human–pig lineage for rotavirus gene 6.

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1995-01-01
2022-01-21
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References

  1. Bellinzoni R. C., Mattion N. M., Matson D. O., Blackhall J., La Torre J. L., Scodeller E. A., Urasawa S., Taniguchi K., Estes M. K. 1990; Porcine rotaviruses antigenically related to human rotavirus serotypes 1 and 2. Journal of Clinical Microbiology 28:633–636
    [Google Scholar]
  2. Both G. W., Siegman L., Bellamy A. R., Ikegami N., Shatkin A. J., Furuichi Y. 1984; Comparative sequence analysis of rotavirus genomic segment 6 – the gene specifying viral subgroups 1 and 2. Journal of Virology 51:97–101
    [Google Scholar]
  3. Estes M. K., Cohen J. 1989; Rotavirus gene structure and function. Microbiological Reviews 53:410–449
    [Google Scholar]
  4. Estes M. K., Mason B. B., Crawford S., Cohen J. 1984; Cloning and nucleotide sequence of the simian rotavirus gene 6 that codes for the major inner capsid protein. Nucleic Acids Research 12:1875–1887
    [Google Scholar]
  5. González S. A., Burrone O. R. 1989; Porcine OSU rotavirus segment 11 sequence shows common features with the viral gene of human origin. Nucleic Acids Research 17:6402
    [Google Scholar]
  6. González S. A., Mattion N. M., Bellinzoni R., Burrone O. R. 1989; Structure of rearranged genome segment 11 in two different rotavirus strains generated by a similar mechanism. Journal of General Virology 70:1329–1336
    [Google Scholar]
  7. Gorziglia M., Hoshino Y., Nishikawa K., Maloy W. L., Jones R. W., Kapikian A. Z., Chanock R. M. 1988; Comparative sequence analysis of the genomic segment 6 of four rotaviruses each with a different subgroup specificity. Journal of General Virology 69:1659–1669
    [Google Scholar]
  8. Greenberg H., McAuliffe V., Valdesuso J., Wyatt R., Flores J., Kalica A., Hoshino Y., Singh N. 1983; Serological analysis of the subgroup protein of rotavirus using monoclonal antibodies. Infection and Immunity 39:91–99
    [Google Scholar]
  9. Hoshino Y., Wyatt R. G., Greenberg H. B., Flores J., Kapikian A. Z. 1984; Serotypic similarity and diversity of rotavirus of mammalian and avian origin as studied by plaque- reduction neutralization. Journal of Infectious Diseases 149:694–702
    [Google Scholar]
  10. Hoshino Y., Gorziglia M., Valdesuso J., Askaa J., Glass R. I., Kapikian A. Z. 1987; An equine rotavirus (FI-14 strain) which bears both subgroup I and subgroup II specificities on its VP6. Virology 157:488–196
    [Google Scholar]
  11. Kapikian A. Z., Cline W. L., Greenberg H. B., Wyatt R. G., Kalica A. R., Banks C. E., James H. D., Flores J., Chanock R. M. 1981; Antigenic characterization of human and animal rotaviruses by immune adherence hemagglutination assay (IAHA); evidence for distinctness of IAHA and neutralization antigens. Infection and Immunity 33:415–425
    [Google Scholar]
  12. Kozak M. 1981; Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Research 9:5233–5252
    [Google Scholar]
  13. López S., Arias C. F. 1993; Sequence analysis of rotavirus YM VP6 and NS28 proteins. Journal of General Virology 74:1223–1226
    [Google Scholar]
  14. Mattion N., González S. A., Burrone O., Bellinzoni R., La Torre J. L., Scodeller E. A. 1988; Rearrangement of genomic segment 11 in two swine rotavirus strains. Journal of General Virology 69:695–698
    [Google Scholar]
  15. Mattion N. M., Cohen J., Estes M. K. 1994; The rotavirus proteins. In Viral Infections of the Gastrointestinal Tract169–249 Kapikian A. Z. New York: Marcel Dekker;
    [Google Scholar]
  16. Ruiz A. M., Lopez I. V., Lopez S., Espejo R. T., Arias C. F. 1988; Molecular and antigenic characterization of porcine rotavirus YM, a possible new rotavirus serotype. Journal of Virology 62:4331–1336
    [Google Scholar]
  17. Saiki R. K., Schaf S., Faloona F., Mullis K. B., Horn G. T., Ehrlich H. A., Arnhein N. 1985; Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230:1350–1354
    [Google Scholar]
  18. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of SciencesUSA 74:5463–5467
    [Google Scholar]
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