1887

Abstract

SUMMARY

Paired serum and cerebrospinal fluid (CSF) samples from 10 AIDS patients with and 10 without AIDS dementia complex (ADC) were studied, in an attempt to uncover ADC-associated variation in V3 sequences. Sequences were obtained from four of the patients with and eight of those without ADC. Comparison of the sequences using a resampling technique revealed a significant ADC-associated difference occurring at several amino acid positions. Results from serum and CSF sequences were comparable. These differences may indicate that the virus found in ADC and that in non-ADC patients have different biological properties. Comparison of serum versus CSF sequences within samples from both ADC and non-ADC patients, using the same resampling technique, revealed no clear distinctions. In some patients, the sequence populations in serum and CSF were completely distinct, while in others, there was no difference in distribution. These patterns were not associated with ADC.

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1995-01-01
2024-11-14
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References

  1. Boom R., Sol C. J. A., Salimans M. M. M., Jansen C. L., Wertheim-Van Dillen P. M. E., Van Der Noordaa J. 1990; Rapid and simple method for purification of nucleic acids. Journal of Clinical Microbiology 28:495–503
    [Google Scholar]
  2. Dayhoff M. O. 1972 Atlas of Protein Sequence and Structure Washington, DC: National Biomedical Research Foundation;
    [Google Scholar]
  3. Delassus S., Cheynier R., Wain-Hobson S. 1992; Non- homogeneous distribution of human immunodeficiency virus type 1 proviruses in the spleen. Journal of Virology 66:5642–5645
    [Google Scholar]
  4. De Jong J. J., Goudsmit J., Keulen W., Klaver B., Krone W. J. A., Tersmette M., De Ronde A. 1992; Human immuno-deficiency virus type 1 clones chimeric for the envelope V3 domain differ in syncytium formation and replication capacity. Journal of Virology 66:757–765
    [Google Scholar]
  5. Eddleston M., De La Torre J. C., Xu J. -Y., Dorfman N., Notkins A., Zolla-Pazner S., Oldstone M. B. A. 1993; Molecular mimicry accompanying HIV-1 infection: human monoclonal antibodies that bind to gp41 and to astrocytes. AIDS Research and Human Retroviruses 9:939–944
    [Google Scholar]
  6. Epstein L. G., Kuiken C. L., Blumberg B. M., Hartman S., Sharer L. R., Clement M., Goudsmit J. 1991; HIV-1 V3 domain variation in brain and spleen of children with AIDS: tissue- specific evolution within host-determined quasispecies. Virology 180:583–590
    [Google Scholar]
  7. Felsenstein J. 1993 PHYLIP manual version 3.5 Berkeley, Calif: University Herbarium of the University of California;
    [Google Scholar]
  8. Fouchier R. A. M., Groenink M., Kootstra N. A., Tersmette M., Huisman H. G., Miedema F., Schuitemaker H. 1992; Phenotype-associated sequence variation in the third variable region of the human immunodeficiency virus type 1 gpl20 molecule. Journal of Virology 66:3183–3187
    [Google Scholar]
  9. Giulian D., Wendt E., Vaca K., Noonan C. A. 1993; The envelope glycoprotein of human immunodeficiency virus type 1 stimulates release of neurotoxins from monocytes. Proceedings of the National Academy of SciencesUSA 90:2769–2773
    [Google Scholar]
  10. Hamming R. W. 1986 Coding and Information Theory, 2. Englewood Cliffs: Prentice Hall;
    [Google Scholar]
  11. Keys B., Karis J., Fadeel B., Valentin A., Norkrans G., Hagberg L., Chiodi F. 1993; V3 sequences of paired HIV-1 isolates from brain and cerebrospinal fluid cluster according to host and show variation related to the clinical stage of disease. Virology 196:475–183
    [Google Scholar]
  12. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitution through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16:111–120
    [Google Scholar]
  13. Koenig S., Gendelman H. E., Orenstein J. M., Dal Canto M. C., Pezeshkpour G. H., Yungbluth M., Janotta F., Aksamit A., Martin M. A., Fauci A. S. 1986; Detection of AIDS virus in macrophages in brain tissue from AIDS patients with encephalo- pathy. Science 233:1089–1093
    [Google Scholar]
  14. Korber B. T. M., Kunstman K., Furtado M., McEvilly M. M., Patterson B., Levy R., Wolinsky S. 1994; HIV-1 sequence differences between blood and simultaneously obtained brain biopsy samples: conserved elements in the V3 region of the envelope protein of brain-derived sequences. Journal of Virology (in press)
    [Google Scholar]
  15. Pang S., Vinters H. V., Akashi T., O’Brien W. A., Chen I. S. Y. 1991; HIV-1 env sequence variation in brain tissue of patients with AIDS-related neurologic disease. Journal of Acquired Immune Deficiency Syndromes 4:1082–1092
    [Google Scholar]
  16. Powers C., McArthur J. C., Johnson R. T., Griffin D. E., Glass J. D., Perryman S., Chesebro B. 1994; Demented and non-demented patients with AIDS differ in brain-derived human immunodeficiency virus type 1 envelope sequences. Journal of Virology 68:4643–1649
    [Google Scholar]
  17. Pulliam L., Herndier B. G., Tang N. M., McGrath M. S. 1991; Human immunodeficiency virus-infected macrophages produce soluble factors that cause histological and chemical alterations in cultured human brains. Journal of Clinical Investigation 87:503–512
    [Google Scholar]
  18. Pulliam L., West D., Haigwood N., Swanson R. A. 1993; HIV- 1 envelope gpl20 alters astrocytes in human brain cultures. AIDS Research and Human Retroviruses 9:439–144
    [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406–425
    [Google Scholar]
  20. Sala M., Zambruno G., Vartanian J. -P., Marconi A., Bertazzoni U., Wain-Hobson S. 1994; Spatial discontinuities in human immunodeficiency virus type 1 quasispecies derived from epidermal Langerhans cells of a patient with AIDS and evidence for double infection. Journal of Virology 68:5280–5283
    [Google Scholar]
  21. Sharpless N., Gilbert D., Vandercam B., Zhou J. M., Verdin E., Ronnett G., Friedman E., Dubois-Dalcq M. 1992; The restricted nature of HIV-1 tropism for cultured neural cells. Virology 191:813–825
    [Google Scholar]
  22. Simmonds P., Zhang L. Q., McOmish F., Balfe P., Ludlam C. A., Leigh Brown A. J. 1991; Discontinuous sequence change of human immunodeficiency virus (HIV) type 1 env sequences in plasma viral and lymphocyte-associated proviral populations in vivo: implications for models of HIV pathogenesis. Journal of Virology 65:6266–6276
    [Google Scholar]
  23. Steuler H., Storch-Hagenlocher B., Wildemann B. 1992; Distinct populations of human immunodeficiency virus type 1 in blood and cerebrospinal fluid. AIDS Research and Human Retroviruses 8:53–59
    [Google Scholar]
  24. Toggas S. M., Masliah E., Rockenstein E. M., Rall G. F., Abraham C. R., Mucke L. 1994; Central nervous system damage produced by expression of the HIV-1 coat protein gpl20 in transgenic mice. Nature 367:188–193
    [Google Scholar]
  25. Trujillo J. R., McLane M. F., Essex M. 1993; Molecular mimicry between the human immunodeficiency virus type 1 gpl20 V3 loop and human brain proteins. Journal of Virologv 67:7711–7715
    [Google Scholar]
  26. Watkins B. A., Dorn H. H., Kelly W. B., Armstrong R. C., Pott B. J., Michaels F., Kufta C. V., Dubois-Dalcq M. 1990; Specific tropism of HIV-1 for microglial cells in primary brain cultures. Science 249:549–553
    [Google Scholar]
  27. Wiley C., Schrier R., Nelson J., Lampert P., Oldstone M. 1986; Cellular localization of HIV infection within brains of AIDS patients. Proceedings of the National Academy of SciencesUSA 83:7089–7093
    [Google Scholar]
  28. Wolfs T. F. W., Zwart G., Barker M., Goudsmit J. 1992; HIV-1 genomic RNA diversification following sexual and parenteral virus transmission. Virology 189:103–110
    [Google Scholar]
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