1887

Abstract

A restriction map of the genomic DNA of phage PL-1 was constructed using the restriction endonucleases HI RI, dIII, I, I and I. The PL-1 genome was 42·2 kb in size and had complementary cohesive ends forming a ring-like monomer. The cohesive ends, analysed with exonuclease III and SI nuclease, were 3 -terminated single strands protruding from both ends. The nucleotide sequence of the cohesive ends, determined by the dideoxynucleotide method, comprised four A + T and 10 G + C pairs: 5′ GAGGCCGACCGTTC 3′ /3′ CTCCGGCTGGCA- AG 5′. Thus, the cohesive ends of PL-1 DNA were higher in G + C content than those of other known bacteriophage DNAs.

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1994-09-01
2021-10-25
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