Nucleotide sequence and genome organization of peanut stripe potyvirus Free

Abstract

A blotch isolate of peanut stripe virus (PStV) was cloned, sequenced and compared with other full-length potyvirus sequences. The viral genome was 10059 nucleotides (nt) in length excluding the poly(A) tail. Two potential AUG start codons were identified in the 5′non-translated region. Analysis of translation products from transcripts containing the first 600 nt of the PStV genome indicated that the first AUG (nt 134 to 136) was preferred over the second AUG (nt 146 to 148) for initiation of translation. Within the single large open reading frame, eight processed proteins were predicted. In general, motifs conserved in other potyviral sequences were also found in the PStV genome. The presence of a 6K protein between the P3 and Cl proteins was predicted. An altered amino acid motif, from FI(V)VRG to FMIIRG, within the carboxyl terminus of the PI protein, separates the PStV sequence from the majority of potyvirus sequences. Based on comparisons with available full-length potyvirus genome sequences, PStV was found to be most closely related to soybean mosaic virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-9-2519
1994-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/9/JV0750092519.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-9-2519&mimeType=html&fmt=ahah

References

  1. Allison R., Johnston R. E., Dougherty W. G. 1986; The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154:9–20
    [Google Scholar]
  2. Boissel J.-P., Kasper T. J., Bunn H. F. 1988; Cotranslational amino-terminal processing of cytosolic proteins: cell-free expression of site-directed mutants of human hemoglobin. Journal of Biological Chemistry 263:8443–8449
    [Google Scholar]
  3. Carrington J. C., Dougherty W. G. 1987; Processing of the tobacco etch virus 49K proteinase requires autoproteolysis. Virology 160:355–362
    [Google Scholar]
  4. Carrington J. C., Dougherty W. G. 1988; A viral cleavage site cassette: identification of amino acid sequences required for tobacco etch virus polyprotein processing. Proceedings of the National Academy of Sciences U.S.A.: 853391–3395
    [Google Scholar]
  5. Carrington J. C., Freed D. D., Oh C.-S. 1990; Expression of potyviral polyproteins in transgenic plants reveals three proteolytic activities required for complete processing. EMBO Journal 9:1347–1353
    [Google Scholar]
  6. Cassidy B., Sherwood J. L., Nelson R. S. 1993; Cloning of the capsid protein gene from a blotch isolate of peanut stripe virus. Archives of Virology 128:287–297
    [Google Scholar]
  7. Domier L. L., Franklin K. M., Shahabuddin M., Hellmann G. M., Overmeyer J. H., Hiremath S. T., Siaw M. F. E., Lomonossoff G. P., Shaw J. G., Rhoads R. E. 1986; The nucleotide sequence of tobacco vein mottling virus RNA. Nucleic Acids Research 14:5417–5430
    [Google Scholar]
  8. Dougherty W. G., Parks T. D. 1991; Post-translational processing of the tobacco etch virus 49-kDa small nuclear inclusion polyprotein: identification of an internal cleavage site and delimitation of VPg and proteinase domains. Virology 183:449–156
    [Google Scholar]
  9. Dougherty W. G., Carrington J. C., Cary S. M., Parks T. D. 1988; Biochemical and mutational analysis of a plant virus polyprotein cleavage site. EMBO Journal 7:1281–1287
    [Google Scholar]
  10. Jayaram CH., Hill J. H., Miller W. A. 1992; Complete nucleotide sequences of two soybean mosaic virus strains differentiated by response of soybean containing the Rsv resistance gene. Journal of General Virology 73:2067–2077
    [Google Scholar]
  11. Johansen E., Rasmussen O. F., Heide M., Borkhardt B. 1991; The complete nucleotide sequence of pea seed-borne mosaic virus RNA. Journal of General Virology 72:2625–2632
    [Google Scholar]
  12. Kamer G., Argos P. 1984; Primary structural comparison of RNA-dependent polymerases from plant, animal and bacterial viruses. Nucleic Acids Research 12:7269–7282
    [Google Scholar]
  13. Kozak M. 1981; Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Research 9:5233–5252
    [Google Scholar]
  14. Laín S., Riechmann J. L., Martin M. T., García J. A. 1989; The complete nucleotide sequence of plum pox potyvirus RNA. Virus Research 13:157–172
    [Google Scholar]
  15. McKern N. M., Mink G. I., Barnett O. W., Mishra L. A., Whittaker M. J., Silbernagel C. W., Ward C. W., Shukla D. D. 1992; Isolates of bean common mosaic virus comprising two distinct potyviruses. Phytopathology 82:923–929
    [Google Scholar]
  16. Maiss E., Timpe U., Brisske A., Jelkmann W., Casper R., Himmler G., Mattanovich D., Katinger H. W. D. 1989; The complete nucleotide sequence of plum pox virus RNA. Journal of General Virology 70:513–524
    [Google Scholar]
  17. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Fourth report of the International Committee on Taxonomy of Viruses. Intervirology 17:1–199
    [Google Scholar]
  18. Mavankal G., Rhoads R. E. 1991; In vitro cleavage at or near the N-terminus of the helper component protein in the tobacco vein mottling virus polyprotein. Virology 185:721–731
    [Google Scholar]
  19. Mierendorf R. C., Pfeffer D. 1987; Sequencing of RNA transcripts synthesized in vitro from plasmids containing bacteriophage promoters. Methods in Enzymology 152:563–566
    [Google Scholar]
  20. Nicolas O., Laliberté J.-F. 1992; The complete nucleotide sequence of turnip mosaic potyvirus RNA. Journal of General Virology 73:2785–2793
    [Google Scholar]
  21. Oh C.-S., Carrington J. C. 1989; Identification of essential residues in potyvirus proteinase HC-Pro by site-directed mutagenesis. Virology 173:692–699
    [Google Scholar]
  22. Puurand U., Makinen K., Paulin L., Saarma M. 1994; The nucleotide sequence of potato virus A genomic RNA and its sequence similarities with other potyviruses. Journal of General Virology 75:457–461
    [Google Scholar]
  23. Reddick B. B., Barnett O. W. 1983; A comparison of three potyviruses by direct hybridization analysis. Phytopathology 73:1506–1510
    [Google Scholar]
  24. Riechmann J. L., Laín S., García J. A. 1992; Highlights and prospects of potyvirus molecular biology. Journal of General Virology 73:1–16
    [Google Scholar]
  25. Robaglia C., Durand-Tardif M., Tronchet M., Boudazin G., Astier-Manifacier S., Casse-Delbart F. 1989; Nucleotide sequence of potato virus Y (N strain) genomic RNA. Journal of General Virology 70:935–947
    [Google Scholar]
  26. Sambrook J., Fritsch E. F., Maniatis T. 1989; . Molecular Cloning: A Laboratory Manual, 2nd edn.. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Strathmann M., Hamilton B. A., Mayeda C. A., Simon M. I., Meyerowitz E. M., Palazzolo M. J. 1991; Transposon- facilitated DNA sequencing. Proceedings of the National Academy of Sciences U.S.A.: 881247–1250
    [Google Scholar]
  28. Thole V., Dalmay T., Burgyan J., Balazs E. 1993; Cloning and sequencing of potato virus Y (Hungarian isolate) genomic RNA. Gene 123:149–156
    [Google Scholar]
  29. Turpen T. 1989; Molecular cloning of a potato virus Y genome: nucleotide sequence homology in non-coding regions of potyviruses. Journal of General Virology 70:1951–1960
    [Google Scholar]
  30. Vance V. B., Moore D., Turpen T. H., Bracker A., Hollowell V. C. 1992; The complete nucleotide sequence of pepper mottle virus genomic RNA: comparison of the encoded polyproteins with those of other sequenced potyviruses. Virology 191:19–30
    [Google Scholar]
  31. Yeh S.-D., Jan F.-J., Chiang C.-H., Doong T.-J., Chen M.-C., Chung P.-H., Bau H.-J. 1992; Complete nucleotide sequence and genetic organization of papaya ringspot virus RNA. Journal of General Virology 73:2531–2541
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-9-2519
Loading
/content/journal/jgv/10.1099/0022-1317-75-9-2519
Loading

Data & Media loading...

Most cited Most Cited RSS feed