RT Journal Article SR Electronic(1) A1 Lawrence, Diane M. A1 Hillman, Bradley I.YR 1994 T1 Synthesis of infectious transcripts of blueberry scorch carlavirus in vitro JF Journal of General Virology, VO 75 IS 9 SP 2509 OP 2512 DO https://doi.org/10.1099/0022-1317-75-9-2509 PB Microbiology Society, SN 1465-2099, AB Blueberry scorch carlavirus (BBScV) is a filamentous virus with a polyadenylated, positive-sense RNA genome. A near full-length cDNA clone of BBScV was constructed by assembly of clones from a cDNA library. To generate a full-length cDNA clone, the 5′ terminus was mutagenized by PCR to introduce nucleotides present in the wild-type virus and not in the near full- length clone, and then fused directly to the T7 bacteriophage RNA polymerase promoter at the 5′ terminus. Capped and uncapped BBScV transcripts were synthesized in vitro from the full-length cDNA clone. Capped transcripts were infectious, producing systemic symptoms identical to those caused by the wild-type virus following inoculation onto Chenopodium quinoa leaves. Uncapped transcripts were substantially less infectious than capped transcripts. This represents the first report of infectious transcripts for a member of the carlavirus group., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-75-9-2509