1887

Abstract

The behaviour of herpes simplex virus type 1 (HSV-1) strain 17 in tissue cultures of PC12 cells treated with nerve growth factor (NGF) was studied. PC12 cells respond to NGF by ceasing to proliferate and extending long neurites. After differentiation with NGF, cultures were infected with HSV-1 and maintained in the presence of the hormone for several weeks. These longterm infected cultures were tested for HSV DNA, transcripts and the ability to produce virus, before and after NGF removal. Before NGF removal, the cultures were characterized by little or no virus production and the presence of HSV-1 DNA in a predominantly endless form. analysis of long-term infected cultures revealed latency-associated transcript expression in only a portion of the cells. However, as shown by an infectious centre assay, virus was present in almost all cells in the population. Moreover, removal of NGF from long-term cultures resulted in the appearance of significantly increased amounts of virus in the media. The degree to which this system resembles HSV latency is discussed.

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1994-09-01
2021-10-21
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References

  1. Block T. M., Spivack J. G., Steiner I., Deshmane S., McIntosh M. T., Lirette R. P., Fraser N. W. 1990; A herpes simplex virus type 1 latency-associated transcript mutant reactivates with normal kinetics from latent infection. Journal of Virology 64:3417–3426
    [Google Scholar]
  2. Cook M. L., Bastone V. B., Stevens J. B. 1974; Evidence that most neurons harbor latent herpes simplex virus. Infection and Immunity 9:945–951
    [Google Scholar]
  3. Croen K. D., Ostrovel J. M., Dragovic L. J., Smialek J. E., Straus S. E. 1987; Latent herpes simplex virus in human trigeminal ganglia: detection of an immediate early gene “antisense” transcript by in situ hybridization. New England Journal of Medicine 317:1427–1432
    [Google Scholar]
  4. Deatly A. M., Spivack J. G., Lavi E., O’Boyle D. R., Fraser N. W. 1988; Latent herpes simplex virus type 1 transcripts in peripheral and central nervous system tissue of mice map to similar regions of the viral genome. Journal of Virology 62:749–756
    [Google Scholar]
  5. Fraser N. W., Spivack J. G., Wroblewska Z., Block T., Deshmane S. L., Valyi-Nagy T., Natarajan R., Gesser R. 1991; A review of the molecular mechanism of HSV-1 latency. Current Eye Research 10:1–13
    [Google Scholar]
  6. Fraser N. W., Block T. M., Spivack J. 1992; The LATs of HSV- 1. Transcripts in search of a function. Virology 191:1–8
    [Google Scholar]
  7. Geller A. I., Breakfield X. O. 1988; A defective HSV-1 vector expresses Escherichia coli β-galactosidase in cultured peripheral neurons. Science 241:1667–1670
    [Google Scholar]
  8. Greene L. A., Rukenstein A. 1989; The quantitative bioassay of nerve growth factor with PC12 cells. In Nerve Growth Factors pp 139–147 Rush R. A. Edited by New York: John Wiley;
    [Google Scholar]
  9. Greene L. A., Tischler A. S. 1976; Establishment of a nonadrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proceedings of the National Academy of Sciences U.S.A.: 732424–2428
    [Google Scholar]
  10. Harris R. A., Preston C. M. 1991; Establishment of latency in vitro by the herpes simplex virus type 1 mutant in 1814. Journal of General Virology 72:907–913
    [Google Scholar]
  11. Mellerick D. M., Fraser N. W. 1987; Physical state of the latent herpes simplex virus genome in a mouse model system: evidence suggesting an episomal state. Virology 158:265–275
    [Google Scholar]
  12. Post L. E., Conley A. J., Mocarski E. S., Roizman B. 1980; Cloning of reiterated and nonreiterated herpes simplex virus 1 sequences as Bam HI fragments. Proceedings of the National Academy of Sciences U.S.A.: 774201–1205
    [Google Scholar]
  13. Rock D. L., Fraser N. W. 1985; , Latent herpes simplex virus type 1 DNA contains two copies of the virion DNA joint region. Journal of Virology 55:849–852
    [Google Scholar]
  14. Roizman B., Sears A. E. 1987; An inquiry into the mechanisms of herpes simplex virus latency. Annual Review of Microbiology 41:543–571
    [Google Scholar]
  15. Rubinstein R., Price R. 1983; Preservation of catecholamine uptake and release in herpes simplex virus type 1-infected PC12 cells. Journal of General Virology 64:2505–2509
    [Google Scholar]
  16. Rubenstein R., Price R. W. 1984; Early inhibition of acetylcholinesterase and choline acetyl transferase activity in herpes simplex virus type 1 infection of PC 12 cells. Journal of Neurochemistry 42:142–150
    [Google Scholar]
  17. Russell J., Preston C. M. 1986; An in vitro latency system for herpes simplex virus type 2. Journal of General Virology 67:397–403
    [Google Scholar]
  18. Stevens J. G., Wagner J., Devi-Rao G. B., Cook M. L., Feldman L. T. 1987; RNA complementary to a herpes virus gene mRNA is prominent in latently infected neurons. Science 235:1056–1059
    [Google Scholar]
  19. Thiele K., Mittnacht S., Kirchner H. 1989; Persistent replication of herpes simplex virus type 1 in JOK-1 cells. Journal of General Virology 70:1907–1911
    [Google Scholar]
  20. Valyi-Nagy T., Deshmane S. L., Raengsakulrach B., Nicosia M., Gesser R. M., Wysocka M., Dillner A., Fraser N. W. 1992; Herpes simplex virus type 1 mutant strain ini814 establishes a unique, slowly progressing infection in SCID mice. Journal of Virology 66:7336–7345
    [Google Scholar]
  21. Wagner E. K., Devi-Rao G., Feldman L. T., Dobson A. T., Zhange Y. F., Hill J. M., Flanagan W. M., Stevens J. G. 1988; Physical characterization of the herpes simplex virus latency- associated transcript in neurons. Journal of Virology 62:1194–1202
    [Google Scholar]
  22. Wigdahl B. L., Zeigler R. J., Sneve M., Rapp F. 1982; Herpes simplex virus latency and reactivation in isolated rat sensory neurons. Virology 127:159–167
    [Google Scholar]
  23. Wilcox C. L., Johnson E. M. 1988; Characterization of nerve growth factor-dependent herpes simplex virus latency in neurons in vitro. Journal of Virology 62:393–399
    [Google Scholar]
  24. Wilcox C. L., Smith R. L., Freed C. R., Johnson E. M. 1990; Nerve growth factor dependence of herpes simplex virus latency in peripheral sympathetic and sensory neurons in vitro . Journal of Neuroscience 10:1268–1275
    [Google Scholar]
  25. Wilcox C. L., Crnic L. S., Pizer L. I. 1992; Replication, latent infection and reactivation in neuronal culture with a herpes simplex virus thymidine kinase negative mutant. Virology 187:348–352
    [Google Scholar]
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