Human T cell leukaemia virus type I-(HTLV-I) transformed cells are capable of stimulating the proliferation of normal T lymphocytes, or stimulating interleukin 2 expression in Jurkat T lymphoid cells. This effect is mediated by the CD2/lymphocyte function-associated antigen 3 (LFA-3) adhesion/signalling pathway. The current work demonstrates that CD3 is also required for this effect, suggesting that the T cell receptor (TCR)/CD3 complex is mediating this effect. However, this effect does not appear to be due to a superantigen since no change in TCR expression was found after HTLV-I-mediated proliferation, nor was proliferation inhibited by an antibody against the specific TCR expressed on Jurkat cells (TCR Vβ8).
BiererB. E., PetersonA., BarbosaB., SeedB., BurakoffS. J.1988; Expression of the T-cell surface molecule CD2 and an epitope-loss CD2 mutant to define the role of lymphocyte function- associated antigen 3 (LFA-3) in T-cell activation. Proceedings of the National Academy of Sciences U.S.A. 85:1194–1198
BockenstedtL. K., GoldsmithM. A., DustinM., OliveD., SpringerT. A., WeissA.1988; The CD2 ligand activates T cells but depends on the expression and function of the antigen receptor. Journal of Immunology 141:1904–1911
CiminaleV., PavlakisG. N., DerseD., CunninghmanC. P., FelberB. K.1992; Complex splicing in the human T-cell leukemia virus (HTLV) family of retroviruses: novel mRNAs and proteins produced by HTLV type I. Journal of Virology 66:1737–1745
DustinM. L., OliveD., SpringerT.1989; Correlation of CD2 binding and functional properties of multimeric and monomeric lymphocyte function-associated antigen 3. Journal of Experimental Medicine 169:503–517
EnglemanE. G., CharronD. J., BenikeC. J., StewartG. J.1980; Ia antigen on peripheral blood mononuclear leukocytes in man. I. Expression, biosynthesis, and function of HLA-DR antigen on non-T cells. Journal of Experimental Medicine 152:99S–113S
FraserJ. D., NewtonM. E., WeissA.1992; CD28 and T cell antigen receptor signal transduction coordinately regulate interleukin 2 gene expression in response to superantigen stimulation. Journal of Experimental Medicine 175:1131–1134
GessainA., LouieA., GoutO., GalloR. C., FranchiniG.1991; Human T-cell leukemia-lymphoma virus type I (HTLV-I) expression in fresh peripheral blood mononuclear cells from patients with tropical spastic paraparesis/HTLV-I-associated myelopathy. Journal of Virology 65:1628–1633
HermanA., CrouteauG., SekalyR., KapplerJ., MarrackP.1990; HLA-DR alleles differ in their ability to present staphylococcal enterotoxins to T cells. Journal of Experimental Medicine 172:709–717
HuginA. W., VacchioM. S., MorseH. C.1991; A virus- encoded ‘ superantigen ’ in a retrovirus-induced immunodeficiency syndrome of mice. Science 252:424–427
HunigT., TiefenthalerG., Meyer Zum BuschenfeldeK. -H., MeuerS. C.1987; Alternative pathway activation of T cells by binding of CD2 to its cell-surface ligand. Nature; London: 326298–301
KimataJ. T., RatnerL.1991; Temporal regulation of viral and cellular gene expression during human T-lymphotropic virus type I- mediated lymphocyte immortalization. Journal of Virology 65:4398–4407
KimataJ. T., PalkerT. J., RatnerL.1993; The mitogenic activity of human T-cell leukemia virus type I is T-cell associated and requires the CD2/LFA-3 activation pathway. Journal of Virology 67:3134–3141
KoralnikI. J., GessainA., KlotmanM. E., MonicoA. L., BernemanZ. N., FranchiniG.1992; Protein isoforms encoded by the pX region of human T-cell leukemia/lymphoma virus type I. Proceedings of the National Academy of Sciences, U.S.A 89:8813–8817
MeuerS. C., HusseyR. E., FabbiM., FoxD., AcutoO., FitzgeraldK. A., HodgdonJ. C., ProtentisJ. P., SchlossmanS. F., ReinherzE. L.1984; An alternative pathway to T-cell activation: a functional role for the 50 kd Til sheep erythrocyte receptor protein. Cell 36:897–906
MoingeonP., LucichJ. L., McConkeyD. J., LetourneurF., MalissenB., KochanJ., ChangH., RodewaldH., ReinherzE. L.1992; CD3f dependence of the CD2 pathway of activation in T lymphocytes and natural killer cells. Proceedings of the National Academy of Sciences, U.S.A 89:1492–1496
Robert-GuroffM., RuscettiF. W., PosnerL. E., PoieszB. J., GalloR. C.1981; Detection of the human T cell lymphoma virus pl9 in cells of some patients with cutaneous T cell lymphoma and leukemia using a monoclonal antibody. Journal of Experimental Medicine 154:1957–1964
TendlerC. L., GreenbergS. J., BlattnerW. A., MannsA., MurphyE., FleisherT., HanchardB., MorganO., BurtonJ. D., NelsonD. L., WaldmannT. A.1990; Transactivation of interleukin 2 and its receptor induces immune activation in human lymphotropic virus type I-associated myelopathy: pathogenic implications and a rationale for immunotherapy. Proceedings of the National Academy of Sciences, U.S.A 87:5218–5222
WucherpfennigK. W., HollsbergP., RichardsonJ. H., BenjaminD., HaflerD. A.1992; T-cell activation by autologous human T-cell leukemia virus type I-infected T-cell clones. Proceedings of the National Academy of Sciences, U.S.A 89:2110–2114