1887

Abstract

Eight isolates of canine distemper virus (CDV) were obtained from seven dogs suffering from distemper by co-cultivation of their mononuclear cells with a marmoset B lymphoblastoid cell line, B95a. Six of the seven dogs had received one or more vaccinations. All of the isolates readily proliferated in B95a cells, but were not completely neutralized by anti-CDV canine plasma, which had high neutralizing activity against the Onderstepoort laboratory strain of CDV. Furthermore, different reactivities of monoclonal antibodies (MAbs) against CDV were observed between the field isolates and laboratory or vaccine strains of CDV in immunofluorescence studies. Immunoprecipitation analysis using MAbs detected the haemagglutinin protein of each new field isolate as 69K, 75K and 155K forms, and the fusion protein as 64K and 65K forms; the corresponding proteins of the Onderstepoort strain were detected as 75K and 61K proteins respectively. It is apparent from these results that the new field isolates of CDV have very different antigenic properties from the Onderstepoort vaccine strain.

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1994-09-01
2024-03-28
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References

  1. Appel M. J. G. 1987; Canine distemper virus. In Virus Infection of Vertebrates 1 Virus Infections of Carnivores pp. 133–159 Horzinek M. C. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  2. Appel M. J. G., Gillespie J. H. 1972; Canine distemper virus. In Virology Monographs 11: pp. 1–96 Gard S., Hallauer C., Meyer K. F. Edited by Wien & New York: Springer-Verlag;
    [Google Scholar]
  3. Barrett T., Visser I. K. G., Mamaev L., Goatlry L., Van Bressen M. -F., Osterhaus A. D. M. E. 1993; Dolphin and porpoise morbilliviruses are genetically distinct from phocine distemper virus. Virology 193:1010–1012
    [Google Scholar]
  4. Campbell J. J., Cosby S. L., Scott J. K., Rima B. K., Martin S. J., Appel M. 1980; A comparison of measles and canine distemper virus polypeptides. Journal of General Virology 48:149–159
    [Google Scholar]
  5. Cattaneo R., Rose J. K. 1993; Cell fusion by the envelope glycoproteins of persistent measles viruses which caused lethal human brain disease. Journal of Virology 67:1493–1502
    [Google Scholar]
  6. Cattaneo R., Schmid A., Spielhofer P., Kaelin K., Baczko K., Ter Meulen V., Pardowitz J., Flanagan S., Rima B. K., Udem S. A., Billeter M. A. 1989; Mutated and hypermutated genes of persistent measles viruses which caused lethal human brain diseases. Virology 173:415–425
    [Google Scholar]
  7. Forthal D. N., Blanding J., Aarnaes S., Peterson E. M., De La Maza L. M., Tilles J. G. 1993; Comparison of different methods and cell lines for isolating measles virus. Journal of Clinical Microbiology 31:695–697
    [Google Scholar]
  8. Hall W. W., Lamb R. A., Choppin P. W. 1980; The polypeptides of canine distemper virus: synthesis in infected cells and relatedness to the polypeptides of other morbilliviruses. Virology 100:433–449
    [Google Scholar]
  9. Hirayama N., Senda M., Yamamoto H., Kurata K., Yoshikawa Y., Yamanouchi K. 1986; Comparison of biological and molecular properties among canine distemper virus strains. Japanese Journal of Veterinary Science 48:259–265
    [Google Scholar]
  10. Hirayama N., Senda M., Nakashima N., Takagi M., Sugiyama M., Yoshikawa Y., Yamanouchi K. 1991; Protective effects of monoclonal antibodies against lethal canine distemper virus infection in mice. Journal of General Virology 72:2827–2830
    [Google Scholar]
  11. Kai C., Ochikubo F., Okita M., Iinuma T., Mikami T., Yamanouchi K. 1993; Use of B95a cells for isolation canine distemper virus from clinical cases. Journal of Veterinary Medical Science 55:1067–1070
    [Google Scholar]
  12. Kobune F., Sakata H., Sugiura A. 1990; Marmoset lympho- blastoid cells as a sensitive host for isolation of measles virus. Journal of Virology 64:700–705
    [Google Scholar]
  13. Kobune F., Sakata H., Sugiyama M., Sugiura A. 1991; B95a, a marmoset lymphoblastoid cell line, as a sensitive host for rinderpest virus. Journal of General Virology 72:687–692
    [Google Scholar]
  14. Örvell C. 1980; Structural polypeptides of canine distemper virus. Archives of Virology 66:193–206
    [Google Scholar]
  15. Örvell C., Norrby E. 1980; Immunological relationships between homologous structural polypeptides of measles and canine distemper virus. Journal of General Virology 50:231–245
    [Google Scholar]
  16. Pringle C. R. 1991; Paramyxoviridae. In Classification and Nomenclature of Viruses.Fifth Report of the International Committee on Taxonomy of Viruses pp. 242–246 Francki R. I. B., Fauquet C. M., Knudson D. L., Brown F. Edited by Wien & New York: Springer-Verlag;
    [Google Scholar]
  17. Rima B. K. 1983; The proteins of morbilliviruses. Journal of General Virology 64:1205–1219
    [Google Scholar]
  18. Rota J. S., Hummel K. B., Rota P. A., Bellini W. J. 1992; Genetic variability of the glycoprotein genes of current wild-type measles isolates. Virology 188:135–142
    [Google Scholar]
  19. Sato T. A., Kohama T., Sugiura A. 1988; Intracellular processing of measles virus fusion protein. Archives of Virology 98:39–50
    [Google Scholar]
  20. Schmid A., Spielhofer P., Cattaneo R., Baczko K., Ter Meulen V., Billeter M. A. 1992; Subacute sclerosing panencephalitis is typically characterized by alterations in the fusion protein cytoplasmic domain of the persisting measles virus. Virology 188:910–915
    [Google Scholar]
  21. Shapshak P., Graves M. C., Imagawa D. T. 1982; Polypeptides of canine distemper virus strains derived from dogs with chronic neurological diseases. Virology 122:158–170
    [Google Scholar]
  22. Sugiyama M., Minamoto N., Kinjo T., Hirayama N., Asano K., Tsukiyama-Kohara K., Yoshikawa Y., Yamanouchi K. 1991; Antigenic and functional characterization of rinderpest virus envelope proteins using monoclonal antibodies. Journal of General Virology 72:1863–1869
    [Google Scholar]
  23. Summers B. A., Greisen H. A., Appel M. J. 1978; Possible initiation of viral encephalomyelitis in dogs by migrating lymphocytes infected with distemper virus. Lancet ii:187–189
    [Google Scholar]
  24. Visser I. K. G., Van Bressem M. -F., De Swart R. L., Van De Bildt M. W. G., Vos H. W., Van Der Heijden R. W. J., Saliki J. T., Örvell C., Kitching P., Kuiken T., Barrett T., Osterhaus A. D. M. E. 1993; Characterization of morbilliviruses isolated from dolphins and porpoises in Europe. Journal of General Virology 74:631–641
    [Google Scholar]
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