1887

Abstract

A marker vaccine elicits an antibody response in the host that can be distinguished from the antibody response induced by a wild-type strain. To obtain a bovine herpesvirus 1 (BHV-1) marker vaccine, we constructed a glycoprotein E (gE) deletion mutant. This was obtained by removing the complete gE coding region from the BHV-1 genome. To attenuate the gE deletion mutant further, we also introduced a small deletion in the thymidine kinase (TK) gene. We selected three mutants: the gE deletion mutant, a TK deletion mutant and a gE/TK double deletion mutant, and examined their virulence and immunogenicity in calves.After intranasal inoculation, the TK deletion mutant showed some residual virulence, whereas the gE and gE/TK deletion mutants were avirulent. The calves inoculated with the deletion mutants were protected against disease after challenge exposure and shed significantly less virus than control calves. Deleting the gE gene, therefore, has little effect on the immunogenicity of BHV-1, but is sufficient to reduce the virulence of BHV-1 in calves. These findings led us to conclude that the gE deletion mutant is a good candidate for a modified live BHV-1 marker vaccine.

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1994-09-01
2021-10-25
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References

  1. Bello L. J., Whitbeck J. C., Lawrence W. C. 1987; Map location of the thymidine kinase gene of bovine herpesvirus 1. Journal of Virology 61:4023–4025
    [Google Scholar]
  2. Bello L. J., Whitbeck J. C., Lawrence W. C. 1992; Sequence and transcript analysis of the bovine herpesvirus 1 thymidine kinase locus. Virology 189:407–414
    [Google Scholar]
  3. Card J. P., Whealy M. E., Robbins A. K., Moore R. Y., Enquist L. W. 1991; Two α-herpesvirus strains are transported differentially in the rodent visual system. Neuron 6:957–969
    [Google Scholar]
  4. Davison A. J., McGeoch D. J. 1986; Evolutionary comparisons of the S segments in the genomes of herpes simplex virus type 1 and varicella-zoster virus. Journal of General Virology 67:597–611
    [Google Scholar]
  5. Gibbs E. P. J., Rweyemamu M. M. 1977; Bovine herpesviruses. Part I. Bovine herpesvirus 1. Veterinary Bulletin 47:317–343
    [Google Scholar]
  6. Gilliam S. E., Thackray A. M., Brown G. A., Field H. J. 1993; The pathogenesis of wild-type and drug resistant mutant strains of bovine herpesvirus-1 (BHV-1) in the natural host. Archives of Virology 128:43–54
    [Google Scholar]
  7. Graham F. L., Van Der Eb A. J. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–467
    [Google Scholar]
  8. Jacobson J. G., Martin S. L., Coen D. M. 1989; A conserved open reading frame that overlaps the herpes simplex virus thymidine kinase gene is important for viral growth in cell culture. Journal of Virology 63:1839–1843
    [Google Scholar]
  9. Kaashoek M. J., Moerman A., Madic J., Rijsewijk F. A. M., Quak J., Gielkens A. L. J., Van Oirschot J. T. 1994; A conventionally attenuated glycoprotein E negative strain of bovine herpesvirus type 1 is an efficacious and safe vaccine. Vaccine 12:439–444
    [Google Scholar]
  10. Kimman T. G., De Wind N., Oei-Lie N., Pol J. M. A., Berns A. J. M., Gielkens A. L. J. 1992a; Contribution of single genes within the unique short region of Aujeszky’s disease virus (suid herpesvirus type 1) to virulence, pathogenesis and immunogenicity. Journal of General Virology 73:243–251
    [Google Scholar]
  11. Kimman T. G., Pol J. M. A., De Wind N., Oei-Lie N., Berns A. J. M., Gielkens A. L. J. 1992b; Role of different genes in the virulence and pathogenesis of Aujeszky’s disease virus. Veterinary Microbiology 33:45–52
    [Google Scholar]
  12. Kit S., Qavi H. 1983; Thymidine kinase (TK) induction after infection of TK-deficient rabbit cell mutants with bovine herpesvirus type 1 (BHV-1): isolation of TK BHV-1 mutants. Virology 130:381–389
    [Google Scholar]
  13. Kit S., Qavi H., Gaines J. D., Billingsley P., McConnell S. 1985; Thymidine kinase-negative bovine herpesvirus type 1 mutant is stable and highly attenuated in calves. Archives of Virology 86:63–83
    [Google Scholar]
  14. Kost T. A., Jones E. V., Smith K. M., Reed A. P., Brown A. L., Miller T. J. 1989; Biological evaluation of glycoproteins mapping to two distinct mRNAs within the BamHl fragment 7 of pseudorabies virus: expression of the coding regions by vaccinia virus. Virology 171:365–376
    [Google Scholar]
  15. Kúdelová M., Kostál M., Cervenáková L., Rajcáni J., Kaerner H. C. 1991; Pathogenicity and latency competence for rabbits of the herpes simplex virus type 1 ANGpath gC and gE defective mutants. Acta virologica 35:438–449
    [Google Scholar]
  16. Mayfield J. E., Good P. J., VanOort H. J., Campbell A. R., Reed D. E. 1983; Cloning and cleavage site mapping of DNA from bovine herpesvirus 1 (Cooper strain). Journal of Virology 47:259–264
    [Google Scholar]
  17. Metzler A. E., Matile H., Gassmann U., Engels M., Wyler R. 1985; European isolates of bovine herpesvirus 1: a comparison of restriction endonuclease sites, polypeptides and reactivity with monoclonal antibodies. Archives of Virology 85:57–69
    [Google Scholar]
  18. Meyer A. L., Petrovskis E. A., Duffus W. P. H., Thomsen D. R., Post L. E. 1991; Cloning and sequence of an infectious bovine rhinotracheitis virus (BHV-1) gene homologous to glycoprotein H of herpes simplex virus. Biochimica et biophysica acta 1090:267–269
    [Google Scholar]
  19. Miller J. M., Van Der Maaten M. J. 1984; Reproductive tract lesions in heifers after intrauterine inoculation with infectious bovine rhinotracheitis virus. American Journal of Veterinary Research 45:790–794
    [Google Scholar]
  20. Pignatti P. F., Cassai E., Meneguzzi G., Chenciner N., Milanesi G. 1979; Herpes simplex virus DNA isolation from infected cells with a novel procedure. Virology 93:260–264
    [Google Scholar]
  21. Rajcáni J., Herget U., Kaerner H. C. 1990a; Spread of herpes simplex virus (HSV) strains SC16, ANG, ANGpath and its glyC minus and glyE minus mutants in DBA-2 mice. Acta virologica 34:305–320
    [Google Scholar]
  22. Rajcáni J., Herget U., Kostál M., Kaerner H. C. 1990b; Latency competence of herpes simplex virus strains ANG, ANGpath and its gC and gE minus mutants. Acta virologica 34:477–486
    [Google Scholar]
  23. Rijsewijk F. A. M., Magdelena J., Moedt J., Kaashoek M. J., Maris-Veldhuis M. A., Gielkens A. L. J., Van Orischot J. T. 1992; Identification and functional analysis of the glycoprotein E (gE) of bovine herpesvirus 1. Proceedings of the 17th International Herpesvirus Workshop, Edinburgh, August 1-7 1992 p. 245
    [Google Scholar]
  24. Rijsewijk F. A. M., Kaashoek M. J., Madic J., Paal H., Ruuls R., Gielkens A. L. J., Van Oirschot J. T. 1993a; Characterization of a DNA rearrangement found in the unique short region of the Za strain of bovine herpesvirus 1 and the vaccine properties of this strain. Proceedings of the 18th International Herpesvirus Workshop, Pittsburg, July 25-30 1993 C67
    [Google Scholar]
  25. Rijsewijk F. A. M., Kaashoek M. J., Moerman A., Madic J., Van Oirschot J. T. 1993b; Marker vaccines and companion diagnostic test offer prospects for eradication of bovine herpesvirus type 1. Proceedings of the IXth International Congress of Virology, Glasgow, August 8-13 1993 p. 230
    [Google Scholar]
  26. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Slater J. D., Gibson J. S., Field H. J. 1993; Pathogenicity of a thymidine kinase-deficient mutant of equine herpesvirus 1 in mice and specific pathogen-free foals. Journal of General Virology 74:819–828
    [Google Scholar]
  28. Spear P. G. 1993; Entry of alphaherpesviruses into cells. Seminars in Virology 4:167–180
    [Google Scholar]
  29. Tikoo S. K., Fitzpatrick D. R., Babiuk L. A., Zamb T. J. 1990; Molecular cloning, sequencing, and expression of functional bovine herpesvirus 1 glycoprotein gIV in transfected bovine cells. Journal of Virology 64:5132–5142
    [Google Scholar]
  30. Van Oirschot J. T., Gielkens A. L. J., Moormann R. J. M., Berns A. J. M. 1990; Marker vaccines, virus protein-specific antibody assays and the control of Aujeszky’s disease. Veterinary Microbiology 23:85–101
    [Google Scholar]
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