Assembled baculovirus-expressed human papillomavirus type 11 L1 capsid protein virus-like particles are recognized by neutralizing monoclonal antibodies and induce high titres of neutralizing antibodies Free

Abstract

Baculovirus-expressed human papillomavirus type 11 (HPV-11) major capsid protein (LI) virus-like particles (VLPs) were produced in insect cells and purified on CsCl density gradients. The VLPs retained conformational neutralizing epitopes that were detected by a series of HPV-11-neutralizing monoclonal antibodies. Electron microscopy determined that the HPV-11 LI

VLPs were variable in size with a surface topography similar to that of infectious HPV-11. The VLPs were very antigenic, and induced high titres of neutralizing antibodies in rabbits and mice when used as an immunogen without commercial preparations of adjuvant. These VLP reagents may be effective vaccines for protection against HPV infections.

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1994-09-01
2024-03-28
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References

  1. Brown C. S., Vanlent J. W. M., Vlak J. M., Spaan W. J. M. 1991; Assembly of empty capsids by using baculovirus recombinants expressing human parvovirus B19 structural proteins. Journal of Virology 65:2702–2706
    [Google Scholar]
  2. Campo M. S., Grindlay G. J., O’Neil B. W., Chandrachud L. M., Mcgarvie G. M., Jarrett W. F. H. 1993; Prophylactic and therapeutic vaccination against a mucosal papillomavirus. Journal of General Virology 74:945–953
    [Google Scholar]
  3. Christensen N. D., Kreider J. W. 1990; Antibody-mediated neutralization in vivo of infectious papillomaviruses. Journal of Virology 64:3151–3156
    [Google Scholar]
  4. Christensen N. D., Kreider J. W. 1991; Neutralization of CRPV infectivity by monoclonal antibodies that identify conformational epitopes on intact virions. Virus Research 21:169–179
    [Google Scholar]
  5. Christensen N. D., Kreider J. W. 1993; Monoclonal antibody neutralization of BPV-1. Virus Research 28:195–202
    [Google Scholar]
  6. Christensen N. D., Kreider J. W., Cladel N. M., Galloway D. A. 1990a; Immunological cross-reactivity to laboratory-produced HPV-11 virions of polysera raised against bacterially-derived fusion proteins and synthetic peptides of HPV-6b and HPV-16 capsid proteins. Virology 175:1–9
    [Google Scholar]
  7. Christensen N. D., Kreider J. W., Cladel N. M., Patrick S. D., Welsh P. A. 1990b; Monoclonal antibody-mediated neutralization of infectious human papillomavirus type 11. Journal of Virology 64:5678–5681
    [Google Scholar]
  8. Christensen N. D., Kreider J. W., Kan N. C., Diangelo S. L. 1991; The open reading frame L2 of cottontail rabbit papillomavirus contains antibody-inducing neutralizing epitopes. Virology 181:572–579
    [Google Scholar]
  9. Cowsert L. M., Lake P., Jenson A. B. 1987; Topographical and conformational epitopes of bovine papillomavirus type 1 defined by monoclonal antibodies. Journal of the National Cancer Institute 79:1053–1058
    [Google Scholar]
  10. Favre M., Breitburd F., Croissant O., Orth G. 1975; Structural polypeptides of rabbit, bovine and human papillomaviruses. Journal of Virology 15:1239–1247
    [Google Scholar]
  11. Firzlaff J. M., Kiviat N. B., Beckmann A. M., Jenison S. A., Galloway D. A. 1988; Detection of human papillomavirus capsid antigens in various squamous epithelial lesions using antibodies directed against the LI and L2 open reading frames. Virology 164:467–477
    [Google Scholar]
  12. French T. J., Marshall J. J. A., Roy P. 1990; Assembly of double-shelled, viruslike particles of bluetongue virus by the simultaneous expression of four structural proteins. Journal of Virology 64:5695–5700
    [Google Scholar]
  13. Ghim S., Christensen N. D., Kreider J. W., Jenson A. B. 1991; Comparison of neutralization of BPV-1 infection of Cl27 cells and bovine fetal skin xenografts. International Journal of Cancer 49:285–289
    [Google Scholar]
  14. Haffar O., Garrigues J., Travis B., Moran P., Zarling J., Hu S. -L. 1990; Human immunodeficiency virus-like, nonreplicating, gag-env particles assemble in a recombinant vaccinia virus expression system. Journal of Virology 64:2653–2659
    [Google Scholar]
  15. Hagensee M. E., Yaegashi N., Galloway D. A. 1993; Selfassembly of human papillomavirus type 1 capsids by expression of the LI protein alone or by coexpression of the LI and L2 capsid proteins. Journal of Virology 67:315–322
    [Google Scholar]
  16. Jarrett W. F. H., O’Neil B. W., Gaukroger J. M., Laird H. M., Smith K. T., Campo M. S. 1990a; Studies on vaccination against papillomaviruses: a comparison of purified virus, tumour extract and transformed cells in prophylactic vaccination. Veterinary Record 126:449–452
    [Google Scholar]
  17. Jarrett W. F. H., O’Neil B. W., Gaukroger J. M., Smith K. T., Laird H. M., Campo M. S. 1990b; Studies on vaccination against papillomaviruses: the immunity after infection and vaccination with bovine papillomaviruses of different types. Veterinary Record 126:473–475
    [Google Scholar]
  18. Jarrett W. F. H., Smith K. T., O’Neil B. W., Gaukroger J. M., Chandrachud L. M., Grindlay G. J., Mcgarvie G. M., Campo M. S. 1991; Studies on vaccination against papillomaviruses: prophylactic and therapeutic vaccination with recombinant structural proteins. Virology 184:33–42
    [Google Scholar]
  19. Jin X. W., Cowsert L., Marshall D., Reed D., Pilacinski W., Lim L. Y., Jenson A. B. 1990; Bovine serological response to a recombinant BPV-1 major capsid protein vaccine. Intervirology 31:345–354
    [Google Scholar]
  20. Jones T. A., Blaug G., Hansen M., Barklis E. 1990; Assembly of gag-β-galactosidase proteins into retrovirus particles. Journal of Virology 64:2265–2279
    [Google Scholar]
  21. Kajigaya S., Fujii H., Field A., Anderson S., Rosenfeld S., Anderson L. J., Shimada T., Young N. S. 1991; Self-assembled B19 parvovirus capsids, produced in a baculovirus system, are antigenically and immunogenically similar to native virions. Proceedings of the National Academy of Sciences U.S.A: 884646–4650
    [Google Scholar]
  22. Kirnbauer R., Booy F., Cheng N., Lowy D. R., Schiller J. T. 1992; Papillomavirus LI major capsid protein self-assembles into virus-like particles that are highly immunogenic. Proceedings of the National Academy of Sciences U.S.A: 8912180–12184
    [Google Scholar]
  23. Kirnbauer R., Taub J., Greenstone H., Roden R., Durst M., Gissmann L., Lowy D. R., Schiller J. T. 1993; Efficient selfassembly of human papillomavirus type 16 LI and L1-L2 into viruslike particles. Journal of Virology 67:6929–6936
    [Google Scholar]
  24. Kreider J. W., Howett M. K., Wolfe S. A., Bartlett G. L., Zaino R. J., Sedlacek T. V., Mortel R. 1985; Morphological transformation in vivo of human uterine cervix with papillomavirus from condylomata acuminata. Nature; London: 317639–641
    [Google Scholar]
  25. Lin Y. -L., Borenstein L. A., Selvakumar R., Ahmed R., Wettstein F. O. 1992; Effective vaccination against papilloma development by immunization with LI or L2 structural protein of cottontail rabbit papillomavirus. Virology 187:612–619
    [Google Scholar]
  26. Lin Y. -L., Borenstein L. A., Ahmed R., Wettstein F. O. 1993; Cottontail rabbit papillomavirus LI protein-based vaccines: protection is achieved only with a full-length, nondenatured product. Journal of Virology 67:4154–4162
    [Google Scholar]
  27. Martínez C., Dalsgaard K., Lópezdeturiso J. A., Cortés E., Vela C., Casal J. I. 1992; Production of porcine parvovirus empty capsids with high immunogenic activity. Vaccine 10:684–690
    [Google Scholar]
  28. Montross L., Watkins S., Moreland R. B., Mamon H., Caspar D. L. D., Garcea R. L. 1991; Nuclear assembly of polyomavirus capsids in insect cells expressing the major capsid protein VP1. Journal of Virology 65:4991–4998
    [Google Scholar]
  29. Olson C. 1963; Cutaneous papillomatosis in cattle and other animals. Annals of the New York Academy of Sciences 108:1042–1056
    [Google Scholar]
  30. Pilacinski W. P., Glassman D. L., Krzyzek R. A., Sadowski P. L., Robbins A. K. 1984; Cloning and expression in Escherichia coli of the bovine papillomavirus LI and L2 open reading frames. BioTechniques 2:356–360
    [Google Scholar]
  31. Rose R. C., Bonnez W., Reichman R. C., Garcea R. L. 1993; Expression of human papillomavirus type 11 LI protein in insect cells: in vivo and in vitro assembly of viruslike particles. Journal of Virology 67:1936–1944
    [Google Scholar]
  32. Rumenapf T., Stark R., Meyers G., Thiel H. -J. 1991; Structural proteins of hog cholera virus expressed by vaccinia virus: further characterization and induction of protective immunity. Journal of Virology 65:589–597
    [Google Scholar]
  33. Saliki J. T., Mizak B., Flore H. P., Gettig R. R., Burand J. P., Carmichael L. E., Wood H. A., Parrish C. R. 1992; Canine parvovirus empty capsids produced by expression in a baculovirus vector: use in analysis of viral properties and immunization of dogs. Journal of General Virology 73:369–374
    [Google Scholar]
  34. Spehner D., Kirn A., Drillien R. 1991; Assembly of nucleocapsidlike structures in animal cells infected with a vaccinia virus recombinant encoding the measles virus nucleoprotein. Journal of Virology 65:6296–6300
    [Google Scholar]
  35. Summers M. D., Smith G. E. 1988; A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agricultural Experiment Station Bulletin1 555:1–56
    [Google Scholar]
  36. Urakawa T., Ferguson M., Minor P. D., Cooper J., Sullivan M., Almond J. W., Bishop D. H. L. 1989; Synthesis of immunogenic, but non-infectious, poliovirus particles in insect cells by a baculovirus expression vector. Journal of General Virology 70:1453–1464
    [Google Scholar]
  37. Zhou J., Sun X. Y., Stenzel D. J., Frazer I. H. 1991; Expression of vaccinia recombinant HPV-16, LI and L2 ORF proteins in epithelial cells is sufficient for assembly of HPV virionlike particles. Virology 185:251–257
    [Google Scholar]
  38. Zhou S., Yang S. Q., Standring D. N. 1992; Characterization of hepatitis B virus capsid particle assembly in Xenopus oocytes. Journal of Virology 66:3086–3092
    [Google Scholar]
  39. Zhou J., Stenzel D. J., Sun X. -Y., Frazer I. H. 1993; Synthesis and assembly of infectious bovine papillomavirus particles in vitro . Journal of General Virology 74:763–768
    [Google Scholar]
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