RT Journal Article SR Electronic(1) A1 Hertig, Christian A1 Pye, Anthony D. A1 Hyatt, Alex D. A1 Boyle, David B.YR 1994 T1 Retrovirus-like particles produced by vaccinia viruses expressing gag-pro-pol region genes of bovine leukaemia virus JF Journal of General Virology, VO 75 IS 9 SP 2213 OP 2221 DO https://doi.org/10.1099/0022-1317-75-9-2213 PB Microbiology Society, SN 1465-2099, AB Processing and assembly of bovine leukaemia virus-like particles were studied in African green monkey kidney cells using recombinant vaccinia viruses (rWs) expressing regions of the bovine leukaemia virus genome. Unprocessed gag precursor protein (Pr44) was detected in immunoblot analysis of lysed cells and particles sedimented from culture supernatants after infection with a rW carrying the gag and truncated protease (pro) gene. Processing of Pr44 was observed after infection of cells with a rW carrying the gag and pro gene or a rW expressing the gag, pro and polymerase (pol) gene. Reverse transcriptase activity was detected only in association with particles produced by gag-,pro- andpol- expressing recombinants. Thin section electron microscopic analysis of infected cells and pelleted particles revealed that Pr44 and processed gag proteins assembled at the cell membrane. Pr44 was released into the cell culture media as immature virus-like particles, whereas processed gag proteins from rWs expressing gag and pro or gag, pro and pol formed mature particles., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-75-9-2213