1887

Abstract

Processing and assembly of bovine leukaemia virus-like particles were studied in African green monkey kidney cells using recombinant vaccinia viruses (rWs) expressing regions of the bovine leukaemia virus genome. Unprocessed gag precursor protein (Pr44) was detected in immunoblot analysis of lysed cells and particles sedimented from culture supernatants after infection with a rW carrying the and truncated protease gene. Processing of Pr44 was observed after infection of cells with a rW carrying the and gene or a rW expressing the and polymerase () gene. Reverse transcriptase activity was detected only in association with particles produced by andexpressing recombinants. Thin section electron microscopic analysis of infected cells and pelleted particles revealed that Pr44 and processed gag proteins assembled at the cell membrane. Pr44 was released into the cell culture media as immature virus-like particles, whereas processed gag proteins from rWs expressing and or and formed mature particles.

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1994-09-01
2024-04-24
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References

  1. Boyle D. B., Coupar B. E. H., Both G. W. 1985; Multiplecloning-site plasmids for the rapid construction of recombinant poxviruses. Gene 35:169–177
    [Google Scholar]
  2. Burny A., Cleuter Y., Kettmann R., Mammerickx M., Marbaix G., Portetelle D., Van Den Broeke A., Willems L., Thomas R. 1988; Bovine leukemia: facts and hypotheses derived from the study of an infectious cancer. Advances in Veterinary Science and Comparative Medicine 32:149–170
    [Google Scholar]
  3. Cann A. J., Chen I. S. Y. 1990; Human T-cell leukemia virus types I and II. In Virology,, 2nd edn.. pp 1501–1527 Fields B. N., Knipe D. M. Edited by New York: Raven Press;
    [Google Scholar]
  4. Chakrabarti S., Brechling K., Moss B. 1985; Vaccinia virus expression vector: coexpression of β-galactosidase provides visual screening of recombinant virus plaques. Molecular and Cellular Biology 5:3403–3409
    [Google Scholar]
  5. Coffin J. M. 1990; Retroviridae and their replication. In Virology,, 2nd edn.. pp 1437–1500 Fields B. N., Knipe D. M. Edited by New York: Raven Press;
    [Google Scholar]
  6. Coulston J., Naif H., Brandon R., Kumar S., Khan S., Daniel R. C. W., Lavin M. F. 1990; Molecular cloning and sequencing of an Australian isolate of proviral bovine leukaemia virus DNA: comparison with other isolates. Journal of General Virology 71:1737–1746
    [Google Scholar]
  7. Davison A. J., Moss B. 1990; New vaccinia virus recombination plasmids incorporating a synthetic late promoter for high level expression of foreign proteins. Nucleic Acids Research 18:4285–4286
    [Google Scholar]
  8. Derse D. 1987; Bovine leukemia virus transcription is controlled by a virus-encoded trans-acting factor and by cis-acting response elements. Journal of Virology 61:2462–2471
    [Google Scholar]
  9. González S. A., Affranchino J. L., Gelderblom H. R., Burny A. 1993; Assembly of the matrix protein of simian immunodeficiency virus into virus-like particles. Virology 194:548–556
    [Google Scholar]
  10. Haffar O., Garrigues J., Travis B., Moran P., Zarling J., Hu S. -L. 1990; Human immunodeficiency virus-like, nonreplicating, gag-env particles assemble in a recombinant vaccinia virus expression system. Journal of Virology 64:2653–2659
    [Google Scholar]
  11. Hoshikawa N., Kojima A., Yasuda A., Takayashiki E., Masuko S., Chiba J., Sata T., Kurata T. 1991; Role of the gag and pol genes of human immunodeficiency virus in the morphogenesis and maturation of retrovirus-like particles expressed by recombinant vaccinia virus: an ultrastructural study. Journal of General Virology 72:2509–2517
    [Google Scholar]
  12. Hu S. -L., Travis B. M., Garrigues J., Zarling J. M., Sridhar P., Dykers T., Eichberg J. W., Alpers C. 1990; Processing, assembly, and immunogenicity of human immunodeficiency virus core antigens expressed by recombinant vaccinia virus. Virology 179:321–329
    [Google Scholar]
  13. Jacks T., Varmus H. E. 1985; Expression of the Rous sarcoma virus pol gene by ribosomal frameshifting. Science 230:1237–1242
    [Google Scholar]
  14. Jenkins S., Gritz L., Fedor C. H., O’Neill E. M., Cohen L. K., Panicali D. L. 1991; Formation of lentivirus particles by mammalian cells infected with recombinant fowlpox virus. AIDS Research and Human Retroviruses 7:991–998
    [Google Scholar]
  15. Karacostas V., Wolffe E. J., Nagashima K., Gonda M. A., Moss B. 1993; Overexpression of the HIV-1 Gag-Pol polyprotein results in intracellular activation of HIV-1 protease and inhibition of assembly and budding of virus-like particles. Virology 193:661–671
    [Google Scholar]
  16. Kräusslich H. -G., Ochsenbauer C., Traenckner A. -M., Mergener K., Fäcke M., Gelderblom H. R., Bosch V. 1993; Analysis of protein expression and virus-like particle formation in mammalian cell lines stably expressing HIV-1 gag and env gene products with or without active HIV proteinase. Virology 192:605–617
    [Google Scholar]
  17. Le S. -Y., Shapiro B. A., Chen J. -H., Nussinov R., Maizel J. V. 1991; RNA pseudoknots downstream of the frameshift sites of retroviruses. Genetic Analysis Techniques and Applications 8:191–205
    [Google Scholar]
  18. Luo L., Li Y., Kang C. Y. 1990; Expression of gag precursor protein and secretion of virus-like gag particles of HIV-2 from recombinant baculovirus-infected insect cells. Virology 179:874–880
    [Google Scholar]
  19. Morikawa S., Booth T. F., Bishop D. H. L. 1991; Analyses of the requirements for the synthesis of virus-like particles by feline immunodeficiency virus gag using baculovirus vectors. Virology 183:288–297
    [Google Scholar]
  20. Nam S. H., Copeland T. D., Hatanaka M., Oroszlan S. 1993; Characterization of ribosomal frameshifting for expression of pol gene products of human T-cell leukemia virus type I. Journal of Virology 67:196–203
    [Google Scholar]
  21. Rasmussen L., Battles J. K., Ennis W. H., Nagashima K., Gonda M. A. 1990; Characterization of virus-like particles produced by a recombinant baculovirus containing the gag gene of the bovine immunodeficiency-like virus. Virology 178:435–451
    [Google Scholar]
  22. Rice N. R., Stephens R. M., Burny A., Gilden R. V. 1985; The gag and pol genes of bovine leukemia virus: nucleotide sequence and analysis. Virology 142:357–377
    [Google Scholar]
  23. Rice N. R., Simek S. L., Dubois G. C., Showalter S. D., Gilden R. V., Stephens R. M. 1987; Expression of the bovine leukemia virus X region in virus-infected cells. Journal of Virology 61:1577–1585
    [Google Scholar]
  24. Shioda T., Shibuta H. 1990; Production of human immunodeficiency virus (HlV)-like particles from cells infected with recombinant vaccinia virus carrying the gag gene of HIV. Virology 175:139–148
    [Google Scholar]
  25. Slabaugh M. B., Roseman N. A. 1989; Retroviral protease-like gene in the vaccinia virus .genome. Proceedings of the National Academy of Sciences, U.S.A.: 864152–4155
    [Google Scholar]
  26. Smith A. J., Srinivasakumar N., Hammarskjöld M. -L., Rekosh D. 1993; Requirements for incorporation of Prl60gag-pol from human immunodeficiency virus type 1 into virus-like particles. Journal of Virology 67:2266–2275
    [Google Scholar]
  27. Van Der Maaten M. J., Miller J. M. 1975; Replication of bovine leukemia virus in monolayer cell cultures. Bibliotheca Haematologica 43:360–362
    [Google Scholar]
  28. Yoshinaka Y., Katoh I., Copeland T. D., Smythers G. W., Oroszlan S. 1986; Bovine leukemia virus protease: purification, chemical analysis, and in vitro processing of gag precursor polyproteins. Journal of Virology 57:826–832
    [Google Scholar]
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